Selected Publications

【Original Articles】

Cafini F, Medrano Romero V, Morikawa K. Mechanisms of Horizontal Gene Transfer in Staphylococcus aureus. InTech, Eds: Shymaa Enany and Laura Elise Crotty Alexander. [Book Chapter]. Accepted 2016.

Cafini F, Nguyen Le TT, Román F, Prieto F, Dubrac S, Msadek T, and Morikawa K. Methodology for The Study of Horizontal Gene Trabsfer in Staphylococcus aureus. JoVE accepted 2016.

Ushijima Y, Yoshida O, Villanueva MJ, Ohniwa RL, Morikawa K. Nucleoid cumping dispensable for the Dps-dependent hydrogen peroxide resistance in Staphylococcus aureus. Microbiology.  doi: 10.1099/mic.0.000353. 2016. pubmed

Medrano Romero V, Morikawa K. Liseria monocytogenes σH contributes to expression of competence genes and intercellular growth. J Bacteriol. 198:1207-1217.  doi: 10.1128/JB.00718-15. 2016. pubmed

Nguyen le TT, Medrano Romero V, Morikawa K. Cell wall-affecting antibiotics modulate natural transformation in SigH-expressing Staphylococcus aureus. J Antibiot (Tokyo). doi: 10. 1038/ja. 132. 2015. pubmed

Cafinii F, Nguyen le TT, Higashide M, Román F, Prieto J, Morikawa K. Horizontal Gene Transmission of cfr gene to MRSA and Enterococcus: role of S. epidermidis as reservoir and alternative pathway for the spread of linezolid resistance. J Antimicrob Chemother. 71 (3):587-92. doi: 10.1093/jac/dkv391.2015. pubmed

Maudsdotter L, Imai S, Ohniwa RL, Saito S, Morikawa K. Staphylococcus aureus dry stress survivors have a heritable fitness advantage in subsequent dry exposure. Microb Infec. 17(6):456-61. doi: 10.1016/j.micinf.2015.02.004. 2015. pubmed

Ushijima Y, Ohniwa RL, Morikawa K. In vitro DNA Protection Assay Using Oxidative Stress. Bio-protocol. 5:1538. 2015. ref

Ushijima Y, Ohniwa RL, Maruyama A, Saito S, Tanaka Y, Morikawa K. Nucleoid compaction by MrgAAsp56Ala/Glu60Ala does not contribute to staphylococcal cell survival against oxidative stress and phagocytic killing by macrophage. FEMSMicrobiol Lett. 360(2):144-51. doi: 10.1111/1574-6968.12598. 2014. pubmed

Vong LB, Yoshitomi T, Morikawa K, Saito S, Matsui H, Nagasaki Y. Oral nanotherapeutics: Effect of redox nanoparticle on microflora in mice with dextran sodium sulfate-induced colitis. J Gastroenterol. 49(5):806-13. doi: 10.1007/s00535-013-0836-8.  2014. pubmed

Ohniwa RL, Muchaku H, Saito S, Wada C, Morikawa K. Atomic force microscopy analysis of the role of major DNA-binding proteins in organization of the nucleoid in Escherichia coli. PLoS One. 8(8):e72954. doi: 10.1371/journal.pone.0072954. eCollection. 2013. pubmed

Masami Ueta, Wada C, Daifuku T, Sako Y, Bessho Y, Kitamura A, Ohniwa RL, Morikawa K, Yoshida H, Kato T, Miyata T, Namba K, Wada A. Conservation of two distinct types of 100S ribosomes in bacteria. Genes Cells. 118(7):554-74. doi: 10.1111/gtc.12057. 2013. pubmed

Morikawa K, Takemura AJ, Inose Y, Tsai M, Nguyen Thi le T, Ohta T, Msadek T. Expression of a cryptic secondary sigma factor gene unveils natural competence for DNA transformation in Staphylococcus aureus. PLoS Pathog. 8(11):e1003003. doi: 10.1371/journal.ppat.1003003. . 2012. pubmed

Ohniwa RL*, Kitabayashi K*, Morikawa K. Alternative cardiolipin synthase Cls1 compensates for stalled Cls2 function in Staphylococcus aureus under conditions of acute acid stress. FEMS Microbiol Lett. 338(2):141-6. doi: 10.1111/1574-6968.12037.  2012. *equal contribution pubmed

Tsai M, Ohniwa RL, Kato Y, Takeshita SL, Ohta T, Saito S, Hayashi H, Morikawa K. Staphylococcus aureus requires cardiolipin for survival under high salinity conditions. BMC Microbiol. 11:13. doi: 10.1186/1471-2180-11-13. 2011. pubmed

Ohniwa RL, Ushijima Y, Saito S,Morikawa K. Proteomic Analyses of Nucleoid-Associated Proteins in Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus. PLoS ONE. 6(4):e19172. doi: 10.1371/journal.pone.0019172. 2011. pubmed

 Morikawa K, Ohniwa RL, Ohta T, Tanaka Y, Takeyasu K, Msadek T. Adaptation beyond the Stress Response: Cell Structure Dynamics and Population Heterogeneity in Staphylococcus aureus. Microbes Environ. 25(2):75-82. Review. 2010. pubmed

Morikawa K, Ohniwa RL, Kumano M, Okamura H, Saito S, Ohta T. The sigH gene sequence can subspeciate staphylococci. Diagn Microbiol Infect Dis. 61(4):373-80. doi: 10.1016/j.diagmicrobio.2008.03.015. 2008. pubmed

Tanaka Y, Morikawa K, Ohki Y, Yao M, Tsumoto K, Watanabe N, Ohta T, Tanaka I. Structural and Mutational analyses of Drp35 from Staphylococcus aureus: a possible mechanism for its lactonase activity. J Biol Chem. 282(8):5770-80. 2007. pubmed

Ohniwa RL, Morikawa K, Takeshita SL, Kim J, Ohta T, Wada C, Takeyasu K. Transcription-coupled Nucleoid Architecture in Bacteria. Genes Cells.12(10):1141-52. 2007. pubmed

Ohniwa RL, Morikawa K, Kim J, Kobori T, Hizume K, Matsumi R, Atomi H, Imanaka T, Ohta T, Wada C, Yoshimura SH, Takeyasu K. Atomic Force Microscopy Dissects the Hierarchy of Genome Architectures in Eukaryote, Prokaryote and Chloroplast. Microsc Microanal. 13(1):3-12. 2007. pubmed

Morikawa K, Ohniwa RL, Kim J, Takeshita SL, Maruyama A, Inose Y, Takeyasu K, Ohta T.. Biochemical, Molecular Genetic, and Structural Analyses of the Staphylococcal Nucleoid. Microsc Microanal. 13(1):30-5. 2007. pubmed

Ohniwa RL, Morikawa K, Kim J, Ohta T, Ishihama A, Wada C, Takeyasu K. Dynamic state of DNA topology is essential for genome condensation in bacteria. EMBO J. 25(23):5591-602. 2006. pubmed

Morikawa K, Ohniwa RL, Kim J, Maruyama A, Ohta T, Takeyasu K. Bacterial nucleoid dynamics: oxidative stress response in Staphylococcus aureus. Genes Cells. 11(4):409-23. 2006. pubmed

Inose Y, Takeshita SL, Hidaka T, Higashide M, Maruyama A, Hayashi H, Morikawa K, Ohta T. Genetic characterization of the natural SigB variants found in clinical isolates of Staphylococcus aureus. J Gen Appl Microbiol. 20(1):35-42. 2006. pubmed

Dorschner RA, Lopez-Garcia B, Peschel A, Kraus D, Morikawa K, Nizet V, Gallo RL. The mammalian ionic environment dictates microbial susceptibility to antimicrobial defense peptides. FASEB J. 20(1):35-42. 2006. pubmed

Morikawa K, Hidaka T, Murakami H, Hayashi H, Ohta T. Staphylococcal Drp35 is the functional counterpart of the eukaryotic PONs. FEMS Microbiol Lett. 249(1):185-90. 2005. pubmed

Zhang H, Morikawa K, Ohta T, Kato Y. In vitro resistance to the CS[alphabeta]-type antimicrobial peptide ASABF-[alpha] is conferred by overexpression of sigma factor sigB in Staphylococcus aureus. J Antimicrob Chemother. 55(5):686-91. 2005. pubmed


【Proceedings】

Yuri Ushijima and Kazuya Morikawa. E. coli Dps without its DNA binding activity (DpsΔ18) can compensate for S. aureus MrgA in hydrogen peroxide resistance. Microbes in the spotlight: recent progress in the understanding of beneficial and harmful microorganisms. 2016.

Aya J Takemura, Yuri Ushijima, Le Thuy Nguyen Thi, and Kazuya Morikawa. Classical rsbU- strains of S. aureus exhibit better growth in synthetic nasal medium. Microbes in the spotlight: recent progress in the understanding of beneficial and harmful microorganisms. 2016.

Le Thuy T. Nguyen, Kazuya Morikawa. “Effects of Antibiotics on Natural Competence in Staphylococcus aureus”. 16th International Symposium on Staphylococci and Staphylococcal Infections. 2014.

Le Thuy Nguyen Thi, Aya J Takemura, Yumiko Inose, Melody Tsai, Toshiko Ohta, Tarek Msadek, and Kazuya Morikawa. First evidences of genetic transformation via natural competence in Staphylococcus aureus. J Bacteriol Parasitol. 114(4): ISSN: 2155-9597 doi: 10.4172/2155-9597.S1.003. 2013.


【Conferences】

Yuri Ushijima, Ryosuke L. Ohniwa, 3, Kazuya Morikawa. E. coli Dps without its DNA binding activity (DpsΔ18) can compensate for the role of S. aureus MrgA in hydrogen peroxide resistance. VI International Conference on Environmental, Industrial, and Applied Microbiology. (BioMicroWorld 2015) Barcelona, Spain. 2015.

Aya J Takemura and Kazuya Morikawa. Reversible mutations in agr locus in Staphylococcus aureus. VI International Conference on Environmental, Industrial, and Applied Microbiology. (BioMicroWorld 2015) Barcelona, Spain. 2015.

Yuri Ushijima, Saki Imai, Aya J Takemura and Kazuya Morikawa. Growth characteristics under in vitro nasal environment are different among strains in Staphylococcus aureus. VI International Conference on Environmental, Industrial, and Applied Microbiology. (BioMicroWorld 2015) Barcelona, Spain. 2015.

Kazuya Morikawa, Nguyen Thi Le Thuy, Aya Takemura, Ryosuke Ohniwa, Tarek Msadek. Natural competence for DNA transformation in Staphylococcus aureus. Life and Medical Sciences in South East Asia. TGSW. Tsukuba, Japan. 2015. oral

Yuri Ushijima, Ryosuke L. Ohniwa, Shinji Saito1, Kazuya Morikawa. A new role of nucleoid dynamics in Staphylococcus aureus. The 9th Tsukuba Medical Science Research Meeting. Tsukuba, Japan. 2015. oral

Aya Takemura, Kazuya Morikawa. A part of Agr-deficient ‘stealth’ Staphylococcus aureus can regain its virulence during phagocytosis. The 9th Tsukuba Medical Science Research Meeting. Tsukuba, Japan. 2015. “outstanding poster awards”

Veronica Medrano Romero, Kazuya Morikawa. Competence regulators in Gram-positive bacteria. 3rd International Congress on Bacteriology and Infectious Diseases. Valencia, Spain. 2015.

竹村彩, 森川一也. 黄色ブドウ球菌は多数の機能未知遺伝子を集団の一部で発現させる. 第88回日本細菌学会総会. Gufu, Japan. 2015.

Lisa Maudsdotter, 今井 沙紀, 森川 一也. S. aureus dry stress survivors have a heritable fitness advantage in subsequent dry exposure. 第88回日本細菌学会総会. Gufu, Japan. 2015.