Ho Lab: Publications

Molecular Parasitology , RNA Modification and Repair


Department of Infection Biology, Faculty of Medicine


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  1. 1.Ho CK and Shuman S. (1996) Physical and Functional Characterization of the Double-Stranded RNA Binding Protein Encoded by the Vaccinia Virus E3 Gene. Virology. 217: 272-284.

  2. 2.Ho CK and Shuman S. (1996) Mutational Analysis of the Vaccinia Virus E3 Protein Defines Amino Acid Residues Involved in E3 Binding to Double-Stranded RNA. J. Virol. 70: 2611-2614.

  3. 3.Ho CK, Van Etten JL, and Shuman S. (1996) Expression and Characterization of an RNA Capping Enzyme Encoded by Chlorella Virus PBCV-1. J. Virol. 70: 6658-6664.

  4. 4.Ho CK, Van Etten JL, and Shuman S. (1997) Characterization of an ATP Dependent DNA Ligase Encoded by Chlorella Virus PBCV-1. J. Virol. 71: 1931-1937.

  5. 5.Doherty AJ, Håkansson K, Ho CK, Shuman S, and Wigley DB. (1997) Purification and Crystallization of the RNA Guanylyltransferase from Chlorella Virus PBCV-1. Acta Cryst. D 53: 482-484.

  6. 6.Wang SP, Deng L, Ho CK, and Shuman S. (1997) Phylogeny of mRNA Capping Enzymes. Proc. Natl. Acad. Sci. USA 94: 9573-9578.

  7. 7.Ho CK, Sriskanda V, McCracken S, Bentley D, Schwer B, and Shuman S. (1998) The Guanylyltransferase Domain of Mammalian mRNA Capping Enzyme Binds to the Phosphorylated Carboxyl-Terminal Domain of RNA Polymerase II. J. Biol. Chem. 273: 9577-9585.

  8. 8.Ho CK, Schwer B, and Shuman S. (1998) Genetic, Physical and Functional Interactions between the Triphosphatase and Guanylyltransferase Components of the Yeast mRNA Capping Apparatus. Mol. Cell. Biol. 18: 5189-5198.

  9. 9.Ho CK, Pei Y, and Shuman S. (1998) Yeast and Viral RNA 5’ Triphosphatase Compromise a New Nucleoside Triphosphatase Family. J. Biol. Chem. 273: 34151-34156.

  10. 10.Ho CK and Shuman S. (1999) Distinct Roles for CTD Ser2 and Ser5 Phosphorylation in the Recruitment and Allosteric Activation of Mammalian mRNA Capping Enzyme. Molecular Cell 3: 405-411.

  11. 11.Lehman K, Schwer B, Ho CK, Rouzankina I, and Shuman S. (1999) A Conserved Domain of Yeast RNA Triphosphatase Flanking the Catalytic Core Regulates Self-association and Interaction with the Guanylyltransferase Component of the mRNA Capping Apparatus. J. Biol. Chem. 274: 22668-22678.

  12. 12.Sriskanda V, Schwer B, Ho CK, and Shuman S. (1999) Mutational Analysis of E.coli DNA Ligase Identifies Amino Acids Required for Nick-Ligation in vitro and for in vivo Complementation of the Growth of Yeast Cells Deleted for CDC9 and LIG4. Nucleic Acid Res. 27: 3953-3963.

  13. 13.Pei Y, Ho CK, Schwer B, and Shuman S. (1999) Mutational Analysis of Yeast RNA Triphosphatases Highlight a Common Mechanism of Metal-dependent NTP Hydrolysis and a Means of Targeting Enzymes to pre-mRNAs in vivo by Fusion to the Guanylyltransferase Component of the Capping Apparatus. J. Biol. Chem. 274: 28865-28874.

  14. 14.Ho CK, Lehman K, and Shuman S. (1999) An Essential Surface Motif of Yeast RNA Triphosphatase Mediates Formation of the mRNA Capping Complex with RNA Guanylyltransferase. Nucleic Acid Res. 27: 4671-4678.

  15. 15. Ho CK, Martins A, and Shuman S. (2000) A Yeast-Based Genetic System for Functional Analysis ofViral mRNA Capping Enzymes. J.Virol. 74: 5486-5494.

  16. 16.Ho CK, Gong C, and Shuman S. (2001) RNA Triphosphatase Component of the mRNA Capping Apparatus of Paramecium bursaria Chlorella Virus 1. J. Virol. 75: 1744-1750.

  17. 17.Ho CK and Shuman S. (2001) A Yeast-like mRNA Capping Apparatus in Plasmodium falciparum.Proc. Natl. Acad. Sci. USA 98: 3050-3055

  18. 18.Chiu YL, Coronel E, Ho CK, Shuman S, and Rana TM. (2001) HIV-1 Tat Protein Interacts with Mammalian Capping Enzyme and Stimulates Capping of TAR RNA. J. Biol. Chem. 276: 12959-12966.

  19. 19.Lehman K, Ho CK, and Shuman S. (2001) Importance of Homodimerization for the in vivo Function of Yeast RNA Triphosphatase. J. Biol. Chem. 276: 14996-15002.

  20. 20.Changela A, Ho CK, Martins A, Shuman S, and Mondragon A. (2001) Structure and Mechanism of the RNA Triphosphatase Component of Mammalian mRNA Capping Enzyme. EMBO J 20: 2575-2586.

  21. 21.Pei Y, Hausmann S, Ho CK, Schwer B, and Shuman S. (2001) The Length, Primary Structure and Phosphorylation State of the RNA Polymerase II CTD Dictate Interactions with the mRNA Capping Enzymes. J. Biol. Chem. 276: 28075-28082.

  22. 22.Hausmann S, Ho CK, Schwer B, and Shuman S. (2001) An Essential Function of Saccharomyces cerevicieae RNA Triphosphatase Cet1 is to Stabilize RNA Guanylyltransferase Ceg1 Against Thermal Inactivation. J. Biol. Chem. 276: 36116-36124.

  23. 23.Ho CK and Shuman S. (2001) Trypanosoma brucei RNA Triphosphatase: Anti-Protozoal Drug Target and Guide to Eukaryotic Phylogeny. J. Biol. Chem. 276:46182-46186.

  24. 24.Ho CK and Shuman S. (2002) Bacteriophage T4 RNA Ligase 2 (gp 24.1) Exemplifies a Family of RNA Ligases Found in All Phylogenic Domains. Proc. Natl. Acad. Sci. USA 99: 12709-12714.

  25. 25.Chiu YL, Ho CK, Saha N, Schwer B, Shuman S, and Rana TM. (2002) Tat Stimulates Cotranscriptional Capping of HIV-1 mRNA. Molecular Cell 10: 585-597.

  26. 26.Yin S, Ho CK, and Shuman S. (2003) Structure-Function Analysis of T4 RNA Ligase 2. J. Biol. Chem. 278: 17601-17608.

  27. 27.Wang LK, Ho CK, Pei Y, and Shuman S. (2003) Mutation Analysis of Bacteriophage T4 RNA Ligase 1: Differential Functional Group are Required for the Nucleotidyl Transfer and Phosphodiester Bond Formation Steps of the Ligation Reaction. J. Biol. Chem. 278: 29454-29462.

  28. 28.Yin S, Ho CK, Miller ES and Shuman S. (2004) Characterization of Bacteriophage KVP40 and T4 RNA Ligase 2. Virology 319: 141-151.

  29. 29.Ho CK, Wang LK, Lima CD and Shuman S. (2004) Structure and Mechanism of RNA Ligase. Structure 12: 327-339.

  30. 30.Schwer B, Sawaya R, Ho CK and Shuman S. (2004) Portability and Fidelity of RNA Repair Systems. Proc. Natl. Acad. Sci. USA 101: 2788-2793

  31. 31.Nandakumar J, Ho CK, Lima CD, and Shuman S. (2004) RNA Substrate Specificity and Structure- Guided Mutational Analysis of T4 RNA Ligase 2. J. Biol. Chem. 279: 31337-31347.

  32. 32.Ho CK, Odell M and Nikolov D. (2004) DNA Ligase Family: Structures. Encyclopedia of Biological Chemistry. Ed. William Lennarz and M. Lane. 4 vols. Academic Press, Elsevier Pub Group.

  33. 33.Pfeffer S, Sewer A, Lagos-Quintana M, Sheridan R, Sander C, Grässer FA, van Dyk LF, Shuman S, Ho CK, Chien M, Russo JJ, Ju J, Randall G, Lindenbach BD, Rice CM, Simon V, Ho DD, Zavolan M, and Tuschl T. (2005) Identification of the MicroRNAs of the Herpesvirus Family. Nature Method 2: 269-276.

  34. 34.Hall MP and Ho CK. (2006) Characterization of a Trypanosoma brucei mRNA Cap (Guanine N-7) Methyltransferase. RNA 12: 488 -497.

  35. 35.Hall MP and Ho CK. (2006) Functional Characterization of a 48-kDa Trypanosoma brucei Cap 2 RNA Methyltransferase. Nucleic Acid Res. 34: 5594 - 5602.

  36. 36.Dong H, Ray D, Ren S, Zhang B, Puig-Basagoiti F, Takagi Y, Ho CK, Li H, and Shi PY. (2007) Distinct RNA Elements Confer Specificity to Flavivirus RNA Cap Methylation Events. J. Virol. 81: 4412-4421.

  37. 37.Takagi Y, Sindkar S, Ekonomidis D, Hall MP and Ho CK. (2007) Trypanosoma brucei Encodes a Bifunctional Capping Enzyme Essential for Cap 4 Formation on the Spliced Leader RNA. J. Biol. Chem. 282: 15995-16005. [link]

  38. 38.Torchea C, Takagi Y and Ho CK. (2008) Archaea RNA Ligase is a Homodimeric Protein that Catalyzes Intramolecular Ligation of Single-Stranded RNA and DNA. Nucleic Acid Res. 36: 6218 - 6227. [link]

  39. 39.Ignatochkina AV, Takagi Y, Liu Y, Nagata K, and Ho CK. (2015) The messenger RNA decapping and recapping pathway in Trypanosoma. Proc. Natl. Acad. Sci. USA 112: 6967-6972 [link]

  40. 40.Mayers T, Purdue BK, Ho CK, Ohneda O. (2015) Teaching science and learning English: an introduction to the Molecular Biology Course in Vietnam. J Med Eng Educ 14(3): 93-98.

  41. 41.Smith P, Ho CK, Takagi Y, Djaballah H, and Shuman S. (2016) Nanomolar Inhibitors of Trypanosoma brucei RNA Triphosphatase. mBIO 7 e00058-16 [link]

  42. 42.Gu H, Yoshinari S, Ghosh R, Ignatochkina AV, Gollnick PD, Murakami KS and Ho CK. (2016) Structural and Mutational Analysis of Archaeal ATP-dependent RNA ligase Identifies Amino Acid Required for RNA Binding and Catalysis. Nucleic Acid Res. 44: 2337-2347 [link]

  43. 43.Ho CK. (2017)  Detection and Identification of Uncapped RNA by Ligation-Mediated Reverse Transcription Polymerase Chain Reaction. mRNA Processing - Methods in Molecular Biology, 1948: 1-9 [link]

  44. 44.Yoshinari S, Liu Y, Gollnick PG and Ho CK. (2017) Cleavage of 3’-terminal adenosine by archaeal ATP-dependent RNA ligase. Scientific Reports 7:11662 [link].

  45. 45.Mayers T, Morikawa K, Ho CK, Ohneda O.(2018) An international exchange program for undergraduate medical science students. Journal of Medical English Education. 17(1) in press