シラバス Syllabus	Doctoral Program in Biomedical Sciences
科目名・科目番号 Subjects・Course Number	02EW401 /   Lecture and Discussion in Molecular Medical Sciences I	02EW403  / Seminar in Molecular Medical Sciences Ⅰ	02EW405  / Practice  in Molecular Medical Sciences I
	02EW402 / Lecture and Discussion in Molecular Medical Sciences II	02EW404  / Seminar in Molecular Medical Sciences II	02EW406　/ Practice  in Molecular Medical Sciences Ⅱ
研究分野 Laboratories	Molecular Cell Biology	Molecular Cell Biology	Molecular Cell Biology
使用言語 Language used （Japanese,  English,  Bilingual）	English	English	English
他研究室学生の受け入れの可否（〇×） Availability for students from other lab.	○ possible	○ possible	TBD upon request.
他研究室学生の問い合わせ先 Contact Information for Students from Other  Lab.	kirie	kirie	kirie
授業形態 Course Type	Lecture, presentation and discussion	Seminar	Practice
標準履修年次 Year	1 or 2	1 or 2	1 or 2
実施学期・曜時限等 Semester,Day and Period	ⅠSpring semester / ⅡAutumn semester	ⅠSpring semester / ⅡAutumn semester	ⅠSpring semester / ⅡAutumn semester
開講場所 Room Number	Conference room, other	Conference room, other	Conference room, other
単位数 Credit	Spring and Autumn semester, 2 x 2 credits	Spring and Autumn semester, 2 x 2 credits	Spring and Autumn semester, 2 x 2 credits
担当教員名・オフィスアワー等	（make an appointment by E-mail）	（make an appointment by E-mail）	（make an appointment by E-mail）
Faculty Members and E-mail	IRIE Kenji: kirie	IRIE Kenji: kirie	IRIE Kenji: kirie
	MIZUNO Tomoaki: mizuno	MIZUNO Tomoaki: mizuno	MIZUNO Tomoaki: mizuno
	SUDA Yasuyuki: ysuda	SUDA Yasuyuki: ysuda	SUDA Yasuyuki: ysuda
授業概要 Course overview	When environmental changes such as temperature and pH or stresses such as nutrient starvation occur in cells, a corresponding cellular response occurs to maintain cell homeostasis. In our laboratory, we use Saccharomyces cerevisiae, a unicellular eukaryote, to control the homeostasis of cells from the viewpoint of “post-transcriptional regulation of gene expression” and “intracellular signaling system”. I am studying the mechanism. Specifically, (1) post-transcriptional regulation of gene expression by RNA-binding proteins in yeast and animal cells, (2) regulation of RNA localization and local translation, (3) regulation of endoplasmic reticulum stress response, (4) We are studying the molecular mechanism of prespore membrane formation by vesicle transport. Through discussions on research content, as a basic researcher in the field of molecular biology, develop the ability to independently plan, conduct, and evaluate experimental research.	By reading abstracts of original papers in English, we understand the contents and standards of leading research on the molecular mechanisms of "post-transcriptional regulation of gene expression" and "intracellular signaling system" or important research that we need to understand, and You will be able to understand your research trends and plan your own creative and valuable research projects. As a basic researcher in molecular biology, develop the ability to plan, conduct and evaluate experimental research based on global trends and standards.	Learn the principles and methods of the experiments required for research on the molecular mechanisms of "post-transcriptional regulation of gene expression" and "intracellular signaling system", and learn safe handling. Develop skills as a basic researcher in molecular biology by acquiring high skills to obtain stable results each time and evaluating the reliability of the results.
授業の到達目標（学修成果） Course Objectives (Learning Outcomes) ←SBO(Specific Behavior Objectives	1. To be able to give a presentation about your own research objectives, methods, results, and future directions. 2. To be able to explain originality and creativity of your own research project. 3. To be able to accept criticisms from other participants to improve your own research project. 4. To be able to understand the presentations ofother speakers, and provideconstructive criticism. 5. To be able to design and perform experiments of recombinant DNA according to the Act concerning recombinant DNA experiment. 6. To be able to design and perform the animal experiments according to the bioethical rule. 7. To build abilities to presentand publish your own experimental results.	1． To develop an ability to select important articles from latest ones by using online search service. 2． To develop an ability to introduce research topics to a general audience in time given. 3． To be able to discuss the unclear points and significances of the articles which are explained by other paticipants. 4． To develop an ability to evaluatethe significance of the selected articles in the related field of research.	1. To be able to perform the identification, staining, motility test, antibiotic sensitivity test, and transformation of pathogenic bacteria. 2. To be able to understand and perform bacteriophage experiments including transduction. 3. To be able to detect and quantify target genes by using PCR and quantitative PCR, respectively. 4. To be able to understand the basis of molecular biology and to construct recombinant genes according to the Act concerning recombinant DNA experiment. 5. To be able to prepare recombinant proteins expressed using appropriate plasmids.  6. To be able to detect specific proteins by Western blotting etc.
キーワード Keyword	Gene, yeast, signal transduction, regulation of gene expression	Gene, yeast, signal transduction, regulation of gene expression	Gene, yeast, signal transduction, regulation of gene expression
授業計画 Course Schedule 第1回（月日、時限）担当教員名　講義内容など	When environmental changes such as temperature and pH or stresses such as nutrient starvation occur in cells, a corresponding cellular response occurs to maintain cell homeostasis. In our laboratory, we use Saccharomyces cerevisiae, a unicellular eukaryote, to control the homeostasis of cells from the viewpoint of “post-transcriptional regulation of gene expression” and “intracellular signaling system”. I am studying the mechanism. Specifically, (1) post-transcriptional regulation of gene expression by RNA-binding proteins in yeast and animal cells, (2) regulation of RNA localization and local translation, (3) regulation of endoplasmic reticulum stress response, (4) We are studying the molecular mechanism of prespore membrane formation by vesicle transport. Through discussions on research content, as a basic researcher in the field of molecular biology, develop the ability to independently plan, conduct, and evaluate experimental research.	By reading abstracts of original papers in English, we understand the contents and standards of leading research on the molecular mechanisms of "post-transcriptional regulation of gene expression" and "intracellular signaling system" or important research that we need to understand, and You will be able to understand your research trends and plan your own creative and valuable research projects. As a basic researcher in molecular biology, develop the ability to plan, conduct and evaluate experimental research based on global trends and standards.	Learn the principles and methods of the experiments required for research on the molecular mechanisms of "post-transcriptional regulation of gene expression" and "intracellular signaling system", and learn safe handling. Develop skills as a basic researcher in molecular biology by acquiring high skills to obtain stable results each time and evaluating the reliability of the results.
履修条件 Course prerequisite	None	None	None
成績評価方法 Grading Criteria	Students are evaluated by the achievement of the objectives listed in the SBO. - Good achieved under supervision in the SBOs 1 and 2:  Pass (C). - Good achieved under supervision in the SBOs 1 - 3, and actively conducted SBO 4:  Pass (B). SBOs 1 - 3 will be evaluated based on a brief report in terms of presentation and dicsussion. - Excellent achievement in SBOs 1-4, and good achievement in SBOs 5-7: (A) - Outstanding achievement with enough evidence in all SBOs : Pass (A+)	Students are evaluated by the achievement of the objectives listed in the SBO. - Good achieved  in the SBOs 1 and 2:  Pass (C). - Good achieved in the SBOs 1 - 2, and actively conducted SBO 3:  Pass (B). Based on written summary of papers (A4 1 page, twice a month).  good achievement including SBO 4 is confirmed,: Pass (A). - Outstanding achievement with enough evidence in all SBOs : Pass (A+)	Skills to obtain reliable experimental results （50%） Understanding of results, and ability to design next experiments will be evaluated by experimental notebook and discussion （50%） A+: Superior (more than 90: top 10%) A: Excellent (80-89: upper 20%) B: Good (70-79) C: Average (60-69) D: Failure (less than 60)
学修時間の割り当て及び授業外における学修方法 Learning method	Lecture 100%	Training (Seminar) 100%	Experiment, Practice 100%
教材・参考文献 Textbook
単位取得要件 Requirement to earn credit	Requirement to earn credit: Attendance 80％ or more. Attendance of 5 hours or more to other lab's classes.	Requirement to earn credit: Attendance 80％ or more. Attendance of 5 hours or more to other lab's classes.	Requirement to earn credit: Attendance 80％ or more.
その他（受講上の注意点等） Notes	Students from Molecular Cell Biology  lab have to attend the course of Practice in Infection Biology. Students should resolve the questions immediately. Students should thoroughly discuss scientifically, logically, and properly.	Students should resolve the questions immediately. Students should thoroughly discuss scientifically, logically, and properly.	Students should contact a responsible faculty member, and get permission beforehand. Students should resolve the questions immediately.
他の授業科目との関連 Relation to Other Courses	Seminar in Molecular Medical Sciences I, II Practice  in Molecular Medical Sciences I, II	Lecture and Discussion in Molecular Medical Sciences I, II Practice  in Molecular Medical Sciences I, II	Lecture and Discussion in Molecular Medical Sciences I, II Seminar in Molecular Medical Sciences I, II

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