シラバス Syllabus	Doctoral Program in Biomedical Sciences
科目名・科目番号 Subjects・Course Number	02EW411 /   Lecture and Discussion in Human Medical Biology I	02EW413  / Seminar in Human Medical Biology	02EW415  / Practice  in Human Medical Biology I
	02EW412 / Lecture and Discussion in Human Medical Biology II	02EW414  / Seminar in Human Medical Biology II	02EW416　/ Practice  in Human Medical Biology Ⅱ
研究分野 Laboratories	Biomedical Engineering	Biomedical Engineering	Biomedical Engineering
使用言語 Language used （Japanese,  English,  Bilingual）	English	English	English
他研究室学生の受け入れの可否（〇×） Availability for students from other lab.	○ possible	○ possible	TBD upon request.
他研究室学生の問い合わせ先 Contact Information for Students from Other  Lab.	hmiyoshi＠md.tsukuba.ac.jp	hmiyoshi＠md.tsukuba.ac.jp	hmiyoshi＠md.tsukuba.ac.jp
授業形態 Course Type	Lecture, presentation and discussion	Seminar	Practice
標準履修年次 Year	1 or 2	1 or 2	1 or 2
実施学期・曜時限等 Semester,Day and Period	ⅠSpring semester / ⅡAutumn semester	ⅠSpring semester / ⅡAutumn semester	ⅠSpring semester / ⅡAutumn semester
開講場所 Room Number	Conference room, other	Conference room, other	Conference room, other
単位数 Credit	Spring and Autumn semester, 2 x 2 credits	Spring and Autumn semester, 2 x 2 credits	Spring and Autumn semester, 2 x 2 credits
担当教員名・オフィスアワー等	（make an appointment by E-mail）	（make an appointment by E-mail）	（make an appointment by E-mail）
Faculty Members and E-mail	MIYOSHI Hiroshi:  hmiyoshi	MIYOSHI Hiroshi:  hmiyoshi	MIYOSHI Hiroshi:  hmiyoshi
	OOKAWA Keiko:  k_ookawa	OOKAWA Keiko:  k_ookawa	OOKAWA Keiko:  k_ookawa
授業概要 Course overview	Students learn skills to design and perform experiments, and evaluate the experimental results through discussing your own results concerning growth and differentiation of cells in the three-dimensional cultures  for developing bioartificial organs.	This is a literature-based, seminar-type course. Students will acquire skills to understand the purpose, methods, and results of latest articles related to tissue engineering and discuss the significances, problems, and future directions of the study.	In this course, students will practically learn methods, experimental principles, and special handling techniques required for the research in tissue engineering and biomedical engineering.
授業の到達目標（学修成果） Course Objectives (Learning Outcomes) ←SBO(Specific Behavior Objectives	1. To be able to give a presentation about your own research objectives, methods, results, and future directions. 2. To be able to explain originality and creativity of your own research project. 3. To be able to accept criticisms from other participants to improve your own research project. 4. To be able to understand the presentations of other speakers, and provide constructive criticism. 5. To be able to design and perform the animal experiments according to the bioethical rule. 6. To be able to design and perform experiments using human cells according to the guidelines. 7. To build abilities to present and publish your own experimental results.	1. To be able to find and select the appropriate paper from major biomedical science journals by using online searching system. 2. To be able to explain the background, outline and described results of the paper within limited time and to answer the other students’ questions. 3. To be able to understand the papers selected by other students by pre-reading and the presentation in the seminar, and make critical discussion on the problems and significance of the work. 4. To be able to explain the historical significance of original papers studied in the seminar in the related research field.	1. To be able to explain the basic techniques for cell cultures, and to be able to perform cells cultures, cryopreservation and microscopic observation. 2. To be able to prepare 3D scaffolds and to seed cells into the scaffolds. 3. To be able to perform 3D cultures and to harvest cells from the scaffolds. 4. To be able to handle laboratory animals (mice and rats) and to harvest cells from these animals. 5. To be able to measure numbers and functions of the 3D cultured cells. 6. To be able to analyze cell specific markers using a flow cytometer. 7. To be able to observe the 3D cultured cells. 8. To be able to analyze, present and discuss experimental data appropriately.
キーワード Keyword	Tissue engineering, bioartificial organs	Tissue engineering, bioartificial organs	Tissue engineering, bioartificial organs
授業計画 Course Schedule 第1回（月日、時限）担当教員名　講義内容など	Weekly, Monday 10:10- Through lecture and discussion series, students acquire knowledge and creativity to set proper research objective, select appropriate methodology,  understand the significance of results, and to further design following experiments independently, concerning growth and differentiation of 3D-cultured cells. In additions, students also learn and confirm general knowledge underlying each specific subjects.	Weekly, Monday 18:00- Class meets every week. Topics to be discussed will be selected by students. Points to be learned include: How to select papers. Histrical background and creativity of good scientific papers. Structure of scientific papers. How to interpretate the results. Logical flow in scientific papers.	Class meets every week. 1. 3D culture techniques 2. Measurement of numbers of 3D cultured cells. 3. Harvesting cells from experimental animals. 4. FACS analysis.
履修条件 Course prerequisite	None	None	None
成績評価方法 Grading Criteria	Grading will be based on the levels of achievements of SBO evaluated by presentations and discussions in the class and the reports on own presentations. ・General achievements in SBO 1-2 with supervisors’ instructions will be evaluated to pass (C or upper). ・ General achievements in SBO 1-3 with supervisors’ instructions and active participation in 4 will be evaluated as B or upper. ・Excellent achievements in SBO 1-4 by students by themselves and successful achievements in 5-7 with supervisors’ instructions will be evaluated as A. ・Excellent achievements in SBO 1-7 by students by themselves will be evaluated as A+.	Grading will be based on the levels of achievements of SBO evaluated by presentations, discussion and submitted reports on the studied papers. ・General achievements in SBO 1-2 with supervisors’ instructions and reports on the studied papers will be evaluated to pass (C or upper). ・General achievements in SBO 1-2 with supervisors’ instructions and reports on the studied papers, and active participation in SBO 3 will be evaluated as B or upper. ・General achievements in SBO 1-3 and excellent reports on the studied papers will be evaluated as A or upper. ・General achievements in SBO 1-4 and excellent reports on the studied papers will be evaluated as A+.	Evaluation by the quality and reliability of the experimental results（50 %） Evaluation of daily notes, ability to explain the research results and planning of next experiments（50%）
学修時間の割り当て及び授業外における学修方法 Learning method	Lecture 100% Self Study: Plan experiments to be done by yourself, select research materials and methods, carry out them, evaluate the results, discuss always with your laboratory colleagues, ask questions to faculty members, and improve your creativity, independency, and performance as a researcher.	Training (Seminar) 100% Self Study: Read the sent papers and extract the discussion points before attending the classes.  Select a latest paper of student's research field, and make a summary once a month.	Experiment, Practice 100% Repeat laboratory techniques and obtain consistent and reliable data.
教材・参考文献 Textbook	『Introduction to Biomedical Engineering』 3rd Edition, JD Enderle, JD Bronzino, eds, Academic Press, 2012  『Principles of Tissue Engineering』 4th Edition, R Lanza, R Langer, JP Vacanti eds, Academic Press, 2013	PDF files of the original paper will be sent to the attendants.	Not specified.
単位取得要件 Requirement to earn credit	Requirement to earn credit: Attendance 80％ or more. Attendance of 5 hours or more to other lab's classes.	Requirement to earn credit: Attendance 80％ or more. Attendance of 5 hours or more to other lab's classes.	Requirement to earn credit: Attendance 80％ or more.
その他（受講上の注意点等） Notes	Take this course with Seminar in Biomedical Engineering. Questions must be asked outright. Discussion must be done thoroughly, scientifically, logically and precisely.	Ÿ Take this course with Lecture in Biomedical Engineering. Ÿ Questions must be asked outright. Ÿ Try to make rigorous, scientific and logical discussion.	We accept registration by the students who select tissue engineering or biomedical engineering for their research field. Registration must be done after approval of the responsible faculty. Questions must be asked outright.
他の授業科目との関連 Relation to Other Courses	Seminar in Human Medical Biology I, II Practice  in Human Medical Biology I, II	Lecture and Discussion in Human Medical Biology I, II Practice  in Human Medical Biology I, II	Lecture and Discussion in Human Medical Biology I, II Seminar in Human Medical Biology I, II

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