シラバス Syllabus Doctoral Program in Biomedical Sciences
科目名・科目番号
Subjects・Course Number
 02EW411 /   Lecture and Discussion in Human Medical Biology I  02EW413  / Seminar in Human Medical Biology  02EW415  / Practice  in Human Medical Biology I
02EW412 / Lecture and Discussion in Human Medical Biology II 02EW414  / Seminar in Human Medical Biology II 02EW416　/ Practice  in Human Medical Biology Ⅱ
研究分野
Laboratories		 Immunology Immunology Immunology
使用言語
Language used （Japanese,  English,  Bilingual）		 English English English
他研究室学生の受け入れの可否（〇×）
Availability for students from other lab.		 ○ possible ○ possible TBD upon request.
他研究室学生の問い合わせ先
Contact Information for Students from Other  Lab.		 kazukos at画像 kazukos at画像 kazukos at画像
授業形態
Course Type		 Lecture, presentation and discussion Seminar Practice 
標準履修年次
Year		 1 or 2 1 or 2 1 or 2
実施学期・曜時限等
Semester,Day and Period		 ⅠSpring semester / ⅡAutumn semester ⅠSpring semester / ⅡAutumn semester ⅠSpring semester / ⅡAutumn semester
開講場所
Room Number		 Conference room, other Conference room, other Conference room, Experimental room, other
単位数
Credit		 Spring and Autumn semester, 2 x 2 credits Spring and Autumn semester, 2 x 2 credits Spring and Autumn semester, 2 x 2 credits
担当教員名・オフィスアワー等 （make an appointment by E-mail） （make an appointment by E-mail） （make an appointment by E-mail）
Faculty Members and E-mail　　　　 SHIBUYA Akira: ashibuya at画像 SHIBUYA Akira: ashibuya at画像 SHIBUYA Akira: ashibuya at画像
SHIBUYA Kazuko: kazukos at画像 SHIBUYA Kazuko: kazukos at画像 SHIBUYA Kazuko: kazukos at画像
TAHARA Satoko: tokothr at画像 TAHARA Satoko: tokothr at画像 TAHARA Satoko: tokothr at画像
ODA Chigusa: chigusano at画像 ODA Chigusa: chigusano at画像 ODA Chigusa: chigusano at画像
NABEKURA Tsukasa: nabekura.tsukasa.fe at画像 NABEKURA Tsukasa: nabekura.tsukasa.fe at画像 NABEKURA Tsukasa: nabekura.tsukasa.fe at画像
授業概要
Course overview		 To establish skills to design, perform and assess the experimental research for immunology as an independent researcher, through discussing withreportsabout normal and abnormal immunological responses revealed by usingnew technologies ingenetic engineering, molecular biology, cell biology and serology and so on.  To understand contents and trend of cutting edge studies on Immunology through critical reading of significant original articles on Immunology and plan unique and important research project for him/her-self. Students study the experimental basis and method to investigate the molecular mechanism of the immune system, and acquire the high-level of experimental techniques to obtain the reproducible results to evaluate the reliability of experiments. This course cultivates basic immunology researchers who have excellent experimental skills and abilities for accurate evaluation of experimental results. 
授業の到達目標（学修成果）
Course Objectives (Learning Outcomes)
←SBO(Specific Behavior Objectives		 1. To be able to understand immune responses at the level of molecules, cells and individuals and explain normal and abnormal immune responses.
2. To be able to present the purpose, methods, results, assessment and future directions ofstudent’s own experimentalresearchprojects.
3. To be able to explain thestage and originality ofstudent’s own experimental research projects in the field.
4. To be able to understand the questions and comments for student’spresentation, and to apply them for improvement of theirresearch activities.
5. To be able to understand the subject and significance of other student’s presentation, and to have discussions with appropriate comments. 		 1. To be able to select a paper to read in the seminar from major biomedical science journals and online searching systems such as PubMed.
2. To be able to explain the background, outline and described results of the paper within limited time and answer the other students’ questions.
3. To be able to understand the papers selected by other students by pre-reading and the presentation in the seminar, and make critical discussion on the problems and significance of the work.
4. To be able to explain the historical significance of original papers studied in the seminar in the related research field. 		 1. To be able to culture, cryopreserve, thaw, observe, and passage cells.
2. To be able to detect the expression pattern of a particular molecule in cell lines or tissues by using fluorescent-labeled antibodies, and analyze and sort cells by flowcytometry
3. To be able to investigate the immune responses by immunizing animals
4. To be able to generate the monoclonal antibodies against a particular molecule.
5. To be able to generate recombinant cDNA in accordance with an intended use with the knowledge of law required for experiment of gene recombination and molecular biology
6. To be able to introduce a recombinant DNA into cell lines, and analyze the expression level by Western-blotting with the knowledge of recombinant DNA vector system by selecting appropriate vector
7. To be able to evaluate the effect of molecules on the cell-proliferation quantitatively
8. To be able to evaluate the effect of molecules on the cytokine production quantitatively 
キーワード
Keyword		 Immune response, Immune disease, Immune response, Immune disease, Immune response, Immune disease,
授業計画
Course Schedule

第1回（月日、時限）担当教員名　講義内容など
 Weekly, Monday 8:30- :  Students present and discuss about their research accomplishment to enable students to build up hypothesis, select appropriate methods, and plan the next experimental approach for the study of immune responses in infection, cancer, autoimmune diseases and allergy. Weekly, Monday 8:30- : Students will critically read significant original papers and discuss on theirtopicsin the papers as well as general remarks in the field. Through this journal club, students understand construction, logic, and originality of scientific research papers and history of the study in the field, and obtain abilities to select significant papers appropriate for this journal club and understand results of scientific research papers. ;    Selection of a paper for our journal club
Composition of biomedical science papers
How to read figures and tables
Logics and coherence of biomedical science papers
Historical values and originality of excellent biomedical science papers		 1. Basic techniquesfor cell-culture
2. Staining cells by using fluorescent-labeled antibodies
3. Flow-cytometric analysis and cell-sorting
4. Construction of recombinant DNA, subcloning, and DNA sequencing
5. Generation of monoclonal antibodies
6. Immuno-precipitation method
7. Western-blotting analysis
8. Quantitative analysis of cytokine production by ELISA and flow-cytometry
9. Quantitative analysis of cell-proliferation by cell-counting and BrdU incorporation 
履修条件
Course prerequisite		 None None None
成績評価方法
Grading Criteria		 Grading will be based on the levels of achievements of SBO evaluated by presentations and discussions in the class and the reports on own presentations.
GeneralachievementsinSBO1-3withsupervisors’instructionswillbeevaluated to pass (C or upper).
GeneralachievementsinSBO1-3withsupervisors’instructionsandactive participation in 4 will be evaluated as B or upper.
ExcellentachievementsinSBO1-4and7bystudentsbythemselvesand successful achievements in 5 and/or 6 with supervisors’ instructions will be evaluated as A.
ExcellentachievementsinSBO1-7bystudentsbythemselveswillbeevaluatedas A+. 		 Grading will be based on the levels of achievements of SBO evaluated by presentations, discussion and 3 submitted reports on the studied papers.
・General achievements in SBO 1-2 with supervisors’ instructions and 3 reports on the studied papers will be evaluated to pass (C or upper).
・General achievements in SBO 1-2 with supervisors’ instructions, active participation in SBO 3 and 3 reports on the studied papers will be evaluated as B or upper.
・General achievements in SBO 1-3 and excellent 3 reports on the studied paperswill be evaluated as A or upper.
・General achievements in SBO 1-4 and excellent 3 reports on the studied papers will be evaluated as A+. 		 Skills to obtain reliable experimental results （50%）
Understanding of results, and ability to design next experiments will be evaluated by experimental notebook and discussion （50%）
A+: Superior (more than 90: top 10%)
A: Excellent (80-89: upper 20%)
B: Good (70-79)
C: Average (60-69)
D: Failure (less than 60)
学修時間の割り当て及び授業外における学修方法
Learning method		 Lecture 100%
Out-of-class study: Read textbooks. Discuss with responsible faculty members and students, and improve own ability for creativity and a sense of independence.		 Training (Seminar) 100%
Out-of-class study:  Write summary on papers (2 per month) together with own opinion.		 Experiment, Practice 100%
教材・参考文献
Textbook		 Cellular and Molecular Immunology Research articles to be discussed for each class will be provided as PDF files.  Basic protocols for experiments will be provided.
単位取得要件
Requirement to earn credit		 Requirement to earn credit: Attendance 80％ or more.
Attendance of 5 hours or more to other lab's classes.		 Requirement to earn credit: Attendance 80％ or more.
Attendance of 5 hours or more to other lab's classes.		 Requirement to earn credit: Attendance 80％ or more.
その他（受講上の注意点等）
Notes		 Take this course with Seminar in Immunology.
Students should actively discussscientifically, logically and minutely.		 Students should resolve the questions immediately.
Students should thoroughly discuss scientifically, logically, and properly.		 Students should contact a responsible faculty member, and get permission beforehand.
Students should resolve the questions immediately.
他の授業科目との関連
Relation to Other Courses		 Seminar in Human Medical Biology I, II
Practice  in Human Medical Biology I, II		 Lecture and Discussion in Human Medical Biology I, II
Practice  in Human Medical Biology I, II		 Lecture and Discussion in Human Medical Biology I, II
Seminar in Human Medical Biology I, II

 

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