シラバス Syllabus	Doctoral Program in Biomedical Sciences
科目名・科目番号 Subjects・Course Number	02EW411 /   Lecture and Discussion in Human Medical Biology I	02EW413  / Seminar in Human Medical Biology	02EW415  / Practice  in Human Medical Biology I
	02EW412 / Lecture and Discussion in Human Medical Biology II	02EW414  / Seminar in Human Medical Biology II	02EW416　/ Practice  in Human Medical Biology Ⅱ
研究分野 Laboratories	Vascular Matrix Biology	Vascular Matrix Biology	Vascular Matrix Biology
使用言語 Language used （Japanese,  English,  Bilingual）	English	English	English
他研究室学生の受け入れの可否（〇×） Availability for students from other lab.	○ possible	○ possible	TBD upon request.
他研究室学生の問い合わせ先 Contact Information for Students from Other  Lab.	hkyanagisawa	hkyanagisawa	hkyanagisawa
授業形態 Course Type	Lecture, presentation and discussion	Seminar	Practice
標準履修年次 Year	1 or 2	1 or 2	1 or 2
実施学期・曜時限等 Semester,Day and Period	ⅠSpring semester / ⅡAutumn semester	ⅠSpring semester / ⅡAutumn semester	ⅠSpring semester / ⅡAutumn semester
開講場所 Room Number	TARA Center B building 2F	TARA Center B building 2F	TARA Center B building 2F
単位数 Credit	Spring and Autumn semester, 2 x 2 credits	Spring and Autumn semester, 2 x 2 credits	Spring and Autumn semester, 2 x 2 credits
担当教員名・オフィスアワー等	（make an appointment by E-mail）	（make an appointment by E-mail）	（make an appointment by E-mail）
Faculty Members and E-mail	YANAGISAWA Hiromi: hkyanagisawa	YANAGISAWA Hiromi: hkyanagisawa	YANAGISAWA Hiromi: hkyanagisawa
	YAMASHIRO Yoshito: yamayoshito	YAMASHIRO Yoshito: yamayoshito	YAMASHIRO Yoshito: yamayoshito
授業概要 Course overview	-Identification and functional analysis of novel extracellular matrix proteins of the vessel wall and its implication in development and disease - Molecular mechanism of mechanotransduction in the vessel wall and aortic aneurysms - Identification of vascular wall stem cells. - Identification of the niche matrix for epidermal stem cells and functional analysis.	This is a literature-based, seminar-type course. Students will acquire skills to understand the purpose, methods, and results of latest articles in the vascular matrix biology  or most-read articles and discuss the significances, problems, and future directions of the study.	Students will practically learn methods, experimental principles, safe handling of mouse and other techniques required for the research in vascular matrix biology.
授業の到達目標（学修成果） Course Objectives (Learning Outcomes) ←SBO(Specific Behavior Objectives	1. To be able to explain the interactions between vascular cells and components of the extracellular microenvironment during normal development, maintenance, and disease progression.  2. To be able to show originality and uniqueness of the research; present aims, methods, results, discussion and significance of the project; and provide immediate plans and future directions. To be able to generate clear and impactful slides and give a logical and lucid presentation.  3. To be able to understand and respond to questions and comments during discussion. To be able to apply comments and advice to one’s research.  4. To be able to understand other researchers’ work and criticize them constructively	1. To be able to select a paper to read in the seminar from major biomedical science journals and online searching systems such as PubMed. 2. To be able to explain the background, outline and described results of the paper within limited time and answer the other students’ questions. 3. To be able to understand the papers selected by other students by prereading and the presentation in the seminar, and make critical discussion on the problems and significance of the work. 4. To be able to explain the historical significance of original papers studied in the seminar in the related research field.	1. To be able to perform cell culture experiments, including passage, observation, freezing and thawing.  2. To be able to culture cells on a matrix and be able to evaluate cell attachment, migration and proliferation.  3. To be able to extract RNA from cells and tissues and perform qPCR analysis. 4. To be able to extract proteins from cells and tissues and perform Western blot analysis.  5. To be able to understand the molecular biological basis of recombinant DNA techniques and have sufficient knowledge on legal restrictions. To be able to perform recombinant DNA experiments.  6. To have sufficient knowledge on vector selections, generate recombinant DNA constructs, transfect into cells, and evaluate the expression by Western blot analysis and/or immunostaining.  7.  To be able to perform breeding, maintenance and genotyping of mouse strains. 8.  To be able to isolate the aorta from the mouse and culture primary vascular cells.
キーワード Keyword	vessels, extracellular matrix, disease	vessels, extracellular matrix, disease	vessels, extracellular matrix, disease
授業計画 Course Schedule 第1回（月日、時限）担当教員名　講義内容など	Fri 9:30-11:00 am /week	Mon 9:30～10:30/week	PCR genotyping, Mouse colony maintenance, including housing, evaluation of mouse phenotype, mouse dissection and aorta harvest, Immunofluorescence staining, Western blotting, sequencing.
履修条件 Course prerequisite	None	None	None
成績評価方法 Grading Criteria	Grading Methods: The students are evaluated by the achievement of SBO. Grading Criteria: A: Excellent (SBO-1 to -4) B: Good (SBO-1 to -3) C: Average (SBO-1 to -2) D: Failure (SBO-1 to -2 or less)	A: Excellent (SBO-1 to 4) B: Good (SBO-1 to 3) C: Average (SBO-1 and 2) D: Failure (SBO-1 only or none)	Attendance and techniques (50%)  - Accurate and timely record keeping with a good understanding of experimental procedures and an ability to plan next experiments (50%)
学修時間の割り当て及び授業外における学修方法 Learning method	Lecture 100% Out-of-class study: Students must always be equipped with up-to-date knowledge in the related fields and be able to incorporate latest technologies. Students must interact with fellow researchers and engage in active discussion on a day-to-day basis.	Training (Seminar) 100% • Make use of Webinars and learn how to give a clear presentation.  • Attend conferences in the relevant field and build connections with other researchers.	Experiment, Practice 100% Repeat laboratory techniques and obtain consistent and reliable data.
教材・参考文献 Textbook	None	Related review articles will be distributed.	Protocols will be provided.
単位取得要件 Requirement to earn credit	Requirement to earn credit: Attendance 80％ or more. Attendance of 5 hours or more to other lab's classes.	Requirement to earn credit: Attendance 80％ or more. Attendance of 5 hours or more to other lab's classes.	Requirement to earn credit: Attendance 80％ or more.
その他（受講上の注意点等） Notes	Students from bacteriology lab have to attend the course of Practice in Infection Biology. Students should resolve the questions immediately. Students should thoroughly discuss scientifically, logically, and properly.	Ÿ Questions must be asked outright. Ÿ Make rigorous, scientific and logical discussion.	Contact faculty members to enroll into the course. Feel free to ask questions at any time
他の授業科目との関連 Relation to Other Courses	Seminar in Human Medical Biology I, II Practice  in Human Medical Biology I, II	Lecture and Discussion in Human Medical Biology I, II Practice  in Human Medical Biology I, II	Lecture and Discussion in Human Medical Biology I, II Seminar in Human Medical Biology I, II

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