シラバス Syllabus Doctoral Program in Biomedical Sciences
科目名・科目番号
Subjects・Course Number
 02EW421 /  Lecture and Discussion in Genome and Environmental Medicine I  02EW423 /  Seminar in Genome and Environmental Medicine I 02EW425 / Practice  in Genome and Environmental Medicine I 
02EW422 / Lecture and Discussion in Genome and Environmental Medicine II 02EW424 /　Seminar in Genome and Environmental Medicine II 02EW426 / Practice  in Genome and Environmental Medicine Ⅱ
研究分野
Laboratories		 Environmental Biology Environmental Biology Environmental Biology
使用言語
Language used （Japanese,  English,  Bilingual）		 Bilingual Bilingual Bilingual
他研究室学生の受け入れの可否（〇×）
Availability for Students from Other Lab.		 ○ ○ ○
他研究室学生の参加に関する問い合わせ先
Contact Information for Students from Other  Lab.		 Yoshito Kumagai,  yk-em-tu at画像 Yoshito Kumagai,  yk-em-tu at画像 Yoshito Kumagai,  yk-em-tu at画像
授業形態
Course Type		 Lecture, presentation and discussion Seminar Practice 
標準履修年次
Year		 1 or 2 1 or 2 1 or 2
実施学期・曜時限等
Semester,Day and Period		 ⅠSpring semester / ⅡAutumn semester
Every Thursday, immediately after “Seminars in Molecular and Genetic Epidemiology” class (approximately 18:15-19:30), except otherwise announced. No class in August, winter and spring breaks.		 ⅠSpring semester / ⅡAutumn semester
Every Thursday, 17:15-18:15,  except otherwise announced. No class in August, winter and spring breaks.		 ⅠSpring semester / ⅡAutumn semester
Intensive
開講場所
Room Number		 Conact the faculty member Conact the faculty member Conact the faculty member
単位数
Credit		 Spring and Autumn semester, 2 x 2 credits Spring and Autumn semester, 2 x 2 credits Spring and Autumn semester, 2 x 2 credits
担当教員名・オフィスアワー等 （make an appointment by E-mail） （make an appointment by E-mail） （make an appointment by E-mail）
Faculty Members and E-mail　　 Yoshito Kumagai,  yk-em-tu at画像 Yoshito Kumagai,  yk-em-tu at画像 Yoshito Kumagai,  yk-em-tu at画像
Yasuhiro Shinkai,  ya_shinkai at画像 Yasuhiro Shinkai,  ya_shinkai at画像 Yasuhiro Shinkai,  ya_shinkai at画像
Yumi Abiko, yumi.abiko at画像 Yumi Abiko, yumi.abiko at画像 Yumi Abiko, yumi.abiko at画像
授業概要
Study Subjects
 This course consists of the progress reports of the attending students. In this course, the students are requested to present their own reearch progress and research plans from the viewpoint of environmental biology, followed by discussion with all students and faculty members. The presentations should be given in English language. Both English and Japanese panguages are used in the discussion.  This  course  is a  journal  club.  Students  are  requested  to  read,  summarize  and  critically review the significance and problems of a recent paper related to environmental biology and  toxicology.  The presentation should be done in English language. Both English and Japanese may be used in the discussion. This course is a workshop on how to use basic databases and how to perform instrumental analysis in environmental biology. Different subjects are provided for Practice in Genome and Environmental Medicine I and II.
授業の到達目標（学修成果）
SBO(Specific Behavior Objectives)		 Students are expected to learn the principles and methodology for  research about environmental biology. As a result, they are expected to acquire the following abilities.

1. The ability to present the purpose, methods, results, interpretation of the results and future research plan.
2.  The ability to to discuss the significance and originality of their experimental analyses in relation to the current understanding in the research field.
3.  The ability to appreciate questions and comments from your peers and advisors, and to effectively utilize them for the improvement of your future research.
4. The ability to understand the significance and limitation of other students’ presentations, and make useful suggestions for the improvement of their research.
5. The ability able to make research protocols in compliance with the Guidelines for bioethics and safety planning under the supervision of faculty members.
6. The ability to explain the inteperetation of data with the usage of appropriate statistical analysis.
7. The ability to propose the appropriate research design in accordance with the characteritics of research object.
8. The ability to explain the social significance of thier own studies adequately.		 By critically reviewing a cutting-edge paper, students will learn the up-to-date researches in the field of environmental biology and toxicology and its implications.  They will also gain an insight how to design and conduct their own studies in order to be able to publish the results in good scientific journals. As a result, they are expected to acquire the following abilities.

1. The ability  to  find  a  suitable  paper  to  review  from  good  scientific  journals  using online reference search systems.
2. The ability to  understand the selected paper, and explain it to other students in a given time period.
3. The ability to ask questions and discuss the problems of the paper presented by other students.
4.  The ability to  explain  the  significance  of  the  paper  in  the  specific  scientific  field. 		 By attending this workshop, the students will learn how to use the databases to obtain necessary information related to their own research projects. They will also learn the basic principles of instrumental analyais which is essential for the studies related to environmental biology. As a result, they are expected to acquire the following abilities. 

"Practice in Genome and Environmental Medicine I"
1. The ability to culture cell line adequately and to evaluate cytotoxicities of environmental chemicals.
2. The ability to evalulate chemical modification of cellular proteins by electrophiles with the usage of biotin-PEAC5-maleimide (BPM) assay.
3. The ability to perform animal experiments adequately and to assess the toxic effects of environmental chemicals on animals.
4. The ability to obtain information on the structure and function of genes and proteins using public databases.

"Practice in Genome and Environmental Medicine II"
5. The ability to design PCR primers and experimental conditions.
6. The ability to prepare gels for electrophoresis analysis of DNA and proteins and to detect protein bands by Western blotting.
7. The ability to analyze environmental chemicals by using HPLC with much knowledge about handling of the machine.
8. The ability to analyze chemicals and proteins by using MS instruments with much knowledge about handling of the machine.
9. The ability to measure metal concentration in biological samples by using ICP-MS with much knowledge about handling of the machine.
キーワード
Keyword		 environmental chemicals, electrophiles, toxicology, environmental response, biological defense, reactive sulfur species, sensor protein, signal trunsduction  environmental chemicals, electrophiles, toxicology, environmental response, biological defense, reactive sulfur species, sensor protein, signal trunsduction  electrophiles, assay of cytotoxicity, BPM assay, PCR, western blotting, HPLC, LC-MS, ICP-MS
授業計画
Course Schedule

第1回（月日、時限）担当教員名　講義内容など
 Friday, 18:15-19:30 (with occasional changes)（right after "Seminar in Genome and Environmental Medicine"）。
Every week, one student makes a presentation on his/her research peogress in English, followed by discussion with peer students and faculty members in English and/or Japanese).		 Friday, 16:00-17:15 (with occasional changes)
Every week, one student critically reviews a journal article related to the molecular and genetic epidemiology fieled in English languiage, followed by discussion with peer students and faculty members in English and/or Japanese.		 intensive
履修条件
Course Prerequisites		 This subject must be taken in combination with "Seminars in Genome and Environmental Medicine". Knowledges on environmental biology analysis of the Master's program level are required to take this course. This subject must be taken in combination with "Lecture and Discussion in Genome and Environmental Medicine". Knowledges on environmental biology of the Master's program level are required to take this course. This subject must be taken in combination with "Lecture and Discussion in Genome and Environmental Medicine" and "Seminar in Genome and Environmental Medicine". Knowledges on environmental biology analysis of the Master's program level are required to take this course.
成績評価方法
Grading Phylosophy		 Grading Methods and Criteria:
Students are evaluated by their  achievement of  SBO.
Students who:
-acdieived SBO 1 and 2 are graded C or higher.
-achieved SBO 1and 2 and actively practicing 3 are graded B or higher.
-achieved 1, 2 and 3, and actively practicing 4-8 are graded A or higher
- exhibited exceptional performance are graded A+ (top 10%).

 Grading Methods and Criteria:
Students are evaluated by their achievement of  SBO
Students who:
-acdieived SBO 1 and 2 are graded C or higher.
-achieved SBO 1and 2 and actively practicing 3 are graded B or higher.
-achieved 1, 2 and 3, and actively practicing 4 are graded A or higher
- exhibited exceptional performance are graded A+ (top 10%).		 Grading Methods and Criteria:
Students are evaluated by the achievement of SBO.
In each of the "Practice in Genome and Environmental Medicine I and II", students who:
-acdieived two  or more SBOs are graded C or higher.
-achieved three or more SBOs are graded B or higher.
-achieved four or more SBOs are graded A or higher
- exhibited exceptional performance are graded A+ (top 10%).
学習時間の割り当て及び授業外における学修方法
Learning method		 Lecture 100% (Students' presentation and discussion)
Out-of-class learning: Preparation for the presentation in the progress report.		 Lecture 100% (Students' presentation and discussion)
Out-of-class learning: Read the distributed paper ahead of the journal club, and think of questions to ask.		 Practice 100%
Out-of-class learning: Use the bioinformatic tools and/or statistical methods in your own study.
教材・参考文献
Textbook		 not rquired Materials will be distributed. Handouts will be distributed,
単位取得要件
Requirement to earn credit		 See "Lecture and Discussion in Genome and Environmental Meedicine I, II") See "Seminar in Genome and Environmental Meedicine I, II") See "Practice in Genome and Environmental Meedicine I, II")
その他（受講上の注意点等）
Notes		 　 　 　
他の授業科目との関連 Seminar in Genome and Environmental MedicineI, II
Practice in Genome and Environmental Medicine I, II		 Lecture and Discussion in Genome and Environmental Medicine I, II
Practice in Genome and Environmental Medicine I, II		 Lecture and Discussion in Genome and Environmental Medicine I, II
Seminar in Genome and Environmental Medicine I, II

 

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