Subjects・Course Number
Laboratories
Language used （Japanese, English, Bilingual）
Availability for students from other lab.
Contact Information for Students from Other Lab.
Course Type
Year
Semester,Day and Period
Room Number
Credit
Course overview
Course Objectives (Learning Outcomes)
←SBO(Specific Behavior Objectives
2. To be able to explain originality and creativity of your own research project.
3. To be able to accept criticisms from other participants to improve your own research project.
4. To be able to understand the presentations ofother speakers, and provideconstructive criticism.
5. To be able to design and perform experiments of recombinant DNA according to the Act concerning recombinant DNA experiment.
6. To be able to design and perform the animal experiments according to the bioethical rule.
7. To build abilities to presentand publish your own experimental results.
2． To develop an ability to introduce research topics to a general audience in time given.
3． To be able to discuss the unclear points and significances of the articles which are explained by other paticipants.
4． To develop an ability to evaluatethe significance of the selected articles in the related field of research.
2. To be able to understand basis of EEG/EMG recording and prepare electrodes for it.
3. To be able to perform streotaxic EEG/EMG electrode implantation surgery.
4. To be able to perform chronic recording of EEG and EMG in free-behaving mice.
5. To be able to analyze data and perform sleep/wake scoring.
6. To be able to prepare mouse brain slices and detect genes of interest by immunohistochemistry etc.
Keyword
Course Schedule
第1回（月日、時限）担当教員名 講義内容など
Through lecture and discussion series, students acquire knowledge and creativity to set proper research objective, select appropriate methodology, understand the significance of results, and to further design following experiments independently, concerning 1) The dynamics of thalamocortical system across sleep/wake cycles2) Neural circuits underlying NREM sleep specific EEG patterns3) Vigilance state-depedent transcriptional changes4) Functions of vigilance-state dependent gene expression in neural activity.
In additions, students also learn and confirm general knowledge underlying each specific subjects.
Class meets every week. Topics to be discussed will be selected by students. Points to be learned include:
How to select papers.
Histrical background and creativity of good scientific papers.
Structure of scientific papers.
How to interpretate the results.
Logical flow in scientific papers.
1. Basic mouse handling
2. Preparation of electrodes for EEG/EMG recording.
3. Stereotaxic electrode implantation surgery
4. Chronic recodring of EEG and EMG in free-behaving mice.
5. Data analysis and sleep/wake scoring.
6. Preparation of mouse brain slices and immunohitochemistry
Course prerequisite
Grading Criteria
- Good achieved under supervision in the SBOs 1 and 2: Pass (C).
- Good achieved under supervision in the SBOs 1 - 3, and actively conducted SBO 4: Pass (B).
SBOs 1 - 3 will be evaluated based on a brief report in terms of presentation and dicsussion.
- Excellent achievement in SBOs 1-4, and good achievement in SBOs 5-7: (A)
- Outstanding achievement with enough evidence in all SBOs : Pass (A+)
- Good achieved in the SBOs 1 and 2: Pass (C).
- Good achieved in the SBOs 1 - 2, and actively conducted SBO 3: Pass (B).
Based on written summary of papers (A4 1 page). good achievement including SBO 4 is confirmed,: Pass (A).
- Outstanding achievement with enough evidence in all SBOs : Pass (A+)
Understanding of results, and ability to design next experiments will be evaluated by experimental notebook and discussion （50%）
A+: Superior (more than 90: top 10%)
A: Excellent (80-89: upper 20%)
B: Good (70-79)
C: Average (60-69)
D: Failure (less than 60)
Learning method
Out-of-class study: Read textbooks. Discuss with responsible faculty members and students, and improve own ability for creativity and a sense of independence.
Out-of-class study: Write summary on papers (2 per month) together with own opinion.
Textbook
Requirement to earn credit
Attendance of 5 hours or more to other lab's classes.
Attendance of 5 hours or more to other lab's classes.
Notes
Students should resolve the questions immediately.
Students should thoroughly discuss scientifically, logically, and properly.
Students should thoroughly discuss scientifically, logically, and properly.
Students should resolve the questions immediately.
Relation to Other Courses
Practice in Medical Science of Sleep I, II
Practice in Medical Science of Sleep I, II
Seminar in Medical Science of Sleep I, II