Subjects・Course Number
Laboratories
Language used （Japanese, English, Bilingual）
Availability for students from other lab.
Contact Information for Students from Other Lab.
Course Type
Year
Semester,Day and Period
Room Number
Credit
Course overview
Course Objectives (Learning Outcomes)
←SBO(Specific Behavior Objectives
2. To be able to explain the position and originality of student’s own research projects in this research field.
3. To be able to understand the questions and comments for student’s presentation, and apply them for improvement of student’s research activities.
4. To be able to understand the significance and defect of other speaker’s presentation, provide constructive criticism, and discuss them.
5. To be able to understand the pathophysiology of kidney and renal diseases at the level of tissue, cells, and molecules, and give the outline.
6. To be able to understand the experimental protocols and the bioethical rules following student’s own research projects, plan, apply, and practice appropriate experiments.
2. To be able to understand the contents of the papers and explain its outline to colleagues within time to discuss.
3. To be able to read the papers on ahead, understand the contents presented by colleagues, and discuss about its significance and query.
4. To be able to explain its background and position of nephrological research.
2. To be able to analyze quantitatively gain-of-function and loss-of-function of cell proliferation.
3. To be able to detect the expression pattern of a particular molecule in cultured cells or tissues with immunofluorescence staining.
4. To be able to fix, dehydrate, embed tissues, and thin-section
5. To be able to detect the expression pattern of a particular molecule in tissues with immunohistochemistry staining including antigen retrieval, optimization of staining, and evaluation of specificity.
6. To be able to construct recombinant cDNA in accordance with an intended use with the knowledge of law required for experiment of gene recombination and molecular biology
7.To be able to analyse and interpret experimental and clinical data using proper statistical methods.
Keyword
Course Schedule
第1回（月日、時限）担当教員名 講義内容など
・Understanding construction of a selected paper
・How to read experimental results
・Logics and coherence of a selected paper
2. Quantitative analysis of cell proliferation by cell counting and BrdU incorporation
3. Immunofluorescence staining of cultured cells
4. Fixation, dehydration, embedding of tissues, and thin-sectioning
5. Immunohistochemical staining (immunofluorescence and immunoenzymatic technique) including antigen retrieval, optimization of staining, and evaluation of specificity
6. Statistical methods and its interpretation.
Course prerequisite
Grading Criteria
- Good achieved under supervision in the SBOs 1 and 2: Pass (C).
- Good achieved under supervision in the SBOs 1 - 3, and actively conducted SBO 4: Pass (B).
SBOs 1 - 3 will be evaluated based on a brief report in terms of presentation and dicsussion.
- Excellent achievement in SBOs 1-4, and good achievement in SBOs 5-7: (A)
- Outstanding achievement with enough evidence in all SBOs : Pass (A+)
- Good achieved in the SBOs 1 and 2: Pass (C).
- Good achieved in the SBOs 1 - 2, and actively conducted SBO 3: Pass (B).
Based on written summary of papers (A4 1 page, twice a month). good achievement including SBO 4 is confirmed,: Pass (A).
- Outstanding achievement with enough evidence in all SBOs : Pass (A+)
Understanding of results, and ability to design next experiments will be evaluated by experimental notebook and discussion （50%）
A+: Superior (more than 90: top 10%)
A: Excellent (80-89: upper 20%)
B: Good (70-79)
C: Average (60-69)
D: Failure (less than 60)
Learning method
Out-of-class study: Read textbooks. Discuss with responsible faculty members and students, and improve own ability for creativity and a sense of independence.
Out-of-class study: Write summary on papers (2 per month) together with own opinion.
Out-of-class study: Learn and acquire stable and reliable experimental techniques.
Textbook
Heptinstall Pathology of the Kidney, 7th Edition, LWW, 2014
Heptinstall Pathology of the Kidney, 7th Edition, LWW, 2014
Heptinstall Pathology of the Kidney, 7th Edition, LWW, 2014
Requirement to earn credit
SOB.
Notes
Students should completely discuss scientifically, logically, and precisely.
Students should completely discuss scientifically, logically, and precisely.
Students should completely discuss scientifically, logically, and precisely.
Related Subjects
Practice in Internal Medicice I, II
Practice in Internal Medicice I, II
Seminar in Internal Medicice I, II