本文へスキップ
Environmental Biology Laboratory Faculty of Medicine University of Tsukuba
環境生物学研究室
Environmental Biology Laboratory　
Faculty of Medicine University of Tsukuba
1-1-1 Tennodai, Tsukuba Ibaraki,
305-8575, Japan

Japanese

Achievement

2016

  1. Nishina T, Deguchi Y, Miura R, Yamazaki S, Shinkai Y, Kojima Y, Okumura K, Kumagai Y, Nakano H. Critical contribution of nuclear factor erythroid 2-related factor 2 (NRF2) to electrophile-induced Interleukin-11 production. Journal of Biological Chemistry 2016; in press.
  2. Abiko Y, Lin FY, Lee H, Puga A, Kumagai Y. Quinone-mediated induction of cytochrome P450 1A1 in HepG2 cells through increased interaction of aryl hydrocarbon receptor with aryl hydrocarbon receptor nuclear translocator. Journal of Toxicological Sciences. 2016; 41: 775-781.
  3. Unoki T, Abiko Y, Toyama T, Uehara T, Tsuboi K, Nishida M, Kaji T, Kumagai Y. Methylmercury, an environmental electrophile capable of activation and disruption of the Akt/CREB/Bcl-2 signal transduction pathway in SH-SY5Y cells. Scientific Reports. 2016; 6: 28944.
  4. Millikin R, Bianco CL, White C, Saund SS, Henriquez S, Sosa V, Akaike T, Kumagai Y, Soeda S, Toscano JP, Lin J, Fukuto JM. The chemicall biology of protein hydropersulfides: Studies of a possible protective function of biological hydropersulfide generateon. Free Radical Biology and Medicine. 2016; 97: 136-147.
  5. Fujiwara Y, Yamamoto C, Yoshida E, Kumagai Y, Kaji T. Heparan sulfate chains potentiate cadmium cytotoxicity in cultured vascular endothelial cells. Archives of Toxicology. 2016; 90: 259-267.
  6. Nishida M, Kumagai Y, Ihara H, Fujii S, Motohashi H, Akaike T. Redox signaling regulated by electrophiles and reactive sulfur species. Journal of Clinical Biochemistry and Nutrition. 2016; 58: 91-98.
  7. Fujie T, Murakami M, Yoshida E, Tachinami T, Shinkai Y, Fujiwara Y, Yamamoto C, Kumagai Y, Naka H, Kaji T. Copper diethyldithiocarbamate as an activator of Nrf2 in cultured vascular endothelial cells. Journal of Biological Inorganic Chemistry. 2016; 21: 263-273.
  8. Shinkai Y, Kimura T, Itagaki A, Yamamoto C, Taguchi K, Yamamoto M, Kumagai Y, Kaji T. Partial contribution of the Keap1-Nrf2 system to cadmium-mediated metallothionein expression in vascular endothelial cells. Toxicology and Applied Pharmacology. 2016; 295: 37-46.
  9. Shinkai Y, Nishihara Y, Amamiya M, Wakayama T, Li S, Kikuch T, Nakai Y, Shimojo N, Kumagai Y. NADPH-cytochrome P450 reductase-mediated denitration reaction of 2,4,6-trinitrotoluene to yield nitrite in mammals. Free Radical Biology and Medicine. 2016; 91: 178-187.

2015

  1. Saund SS, Sosa V, Henriquez, Nguyen QNN, Soeda S, Bianco CL, Millikin R, White C, Le H, Ono K, Tantillo DJ, Kumagai Y, Akaike T, Lin J, Fukuto JM. The Chemical Biology of Hydropersulfides (RSSH): Chemical Stability, Reactivity and Redox Roles. Archives Biochemistry and Biophysics. 2015; 588: 15-24.
  2. Toyama T, Abiko Y, Katayama Y, Kaji T, Kumagai Y. S-Mercuration of ubiquitin C-terminal hydrolase L1 through Cys152 by methylmercury causes inhibition of its catalytic activity and reduction of monoubiquitin levels in SH-SY5Y cells. Journal of Toxicological Sciences. 2015; 40: 887-893.
  3. Abiko Y, Puga A, Kumagai Y. Covalent binding of quinones activates the Ah receptor in Hepa1c1c7 cells. Journal of Toxicological Sciences. 2015; 40: 873-886.
  4. Ishii I, Kamata S, Hagiya Y, Abiko Y, Kasahara T, Kumagai Y. Protective effects of hydrogen sulfide anions against acetaminophen-induced hepatotoxicity in mice. Journal of Toxicological Sciences. 2015;40: 837-841.
  5. Shinkai Y, Li S, Kikuchi T, Shimojo N, Kumagai Y. Health survey of workers in a 2,4,6-trinitrotoluene explosives factory in Fuxin, China. Fundamental Toxicological Sciences. 2015; 2: 171-175.
  6. Abiko Y, Ishii I, Kamata S, Tsuchiya Y, Watanabe Y, Ihara H, Akaike T, Kumagai Y. Formation of sulfur adducts of N-acetyl-p-benzoquinoneimine, an electrophilic metabolite of acetaminophen in vivo: Participation of reactive persulfides. Chemical Research in Toxicology. 2015; 28: 1796-1802.
  7. Shinkai Y, Li S, Kikuchi T, Kumagai Y. Participation of metabolic activation of 2,4,6-trinitrotoluene to 4-hydroxylamino-2,6-dinitrotoluene in the hematotoxicity. Journal of Toxicological Sciences. 2015; 40: 597-604.
  8. 熊谷嘉人、安孫子ユミ. レドックスサイクルを介して酸化ストレスを生じる大気中成分. 別冊「医学のあゆみ」レドックスUPDATE 2015; 312-317.
  9. 熊谷嘉人、内田浩二. RSSによる環境中親電子物質の解毒代謝. 細胞工学「活性イオウ分子種の生理機能に迫る　チオールバイオロジーの新たなステージ」 2015; 34: 358-363.
  10. Abiko Y, Yoshida E, Ishii I, Fukuto JM, Akaike T, Kumagai Y. Involvement of reactive persulfides in biological bismethylmercury sulfide formation. Chemical Research in Toxicology. 2015; 28: 1301-1306.
  11. Abiko Y, Luong C N, Kumagai Y. A Biotin-PEAC5-maleimide labeling assay to detect electrophiles. Journal of Toxicological Sciences. 2015; 40: 405-411.
  12. Shinkai Y, Abiko Y, Ida T, Miura T, Kakehashi H, Ishii I, Nishida M, Sawa T, Akaike T, Kumagai Y. Reactive sulfur species-mediated activation of the Keap1-Nrf2 pathway by 1,2-napthoquinone through sulfenic acids formation under oxidative stress. Chemical Research in Toxicology. 2015; 28: 838-847.
  13. Nakano S, Ishii I, Shinmura K, Tamaki K, Hishiki T, Akahoshi N, Ida T, Nakanishi T, Kamata S, Kumagai Y, Akaike T, Fukuda K, Sano M, Suematsu M. Hyperhomocysteinemia abrogates fasting-induced cardioprotection against ischemia/reperfusion by limiting bioavailability of hydrogen sulfide anions. Journal of Molecular Medicine. 2015; 93: 879-889.
  14. Hagiya Y, Kamata S, Mitsuoka S, Okada N, Yoshida S, Yamamoto J, Ohkubo R, Abiko Y, Yamada H, Akahoshi N, Kasahara T, Kumagai Y, Ishii I. Hemizygosity of transsulfuration genes confers increased vulnerability against acetaminophen-induced hepatotoxicity in mice. Toxicology and Applied Pharmacology. 2015; 282: 195-206.
  15. Makino K, Okuda K, Sugino E, Nishiya T, Toyama T, Iwawaki T, Fujimura M, Kumagai Y, Uehara T. Correlation between attenuation of protein disulfide isomerase activity through S-mercuration and neurotoxicity induced by methyl mercury. Neurotoxicology Research. 2015; 27: 99-105.

2014

  1. 西田基宏、外山喬士、熊谷嘉人、富田拓郎．活性硫黄種によるレドックス恒常性維持機構に基づいた新規心不全治療戦略の構築．YAKUGAKAU ZASSHI. 2014; 134: 1239-1243.
  2. Abiko Y, Mizokawa M, Kumagai Y. Activation of the Keap1/Nrf2 pathway through covalent modification of the 2-alkenal group of aliphatic electrophiles in Coriandrum sativum L. Journal of Agricultural and Food Chemistry. 2014; 62: 10936-10944.
  3. Ono K, Akaike T, Sawa T, Kumagai Y, Wink DA, Tantillo DJ, Hobbs AJ, Nagy P, Xian M, Lin J, Fukuto JM. The redox chemistry and chemical biology of H2S, hydropersulfides and derived species: Implication to their possible biological activity and utility. Free Radical Biology & Medicine. 2014; 77: 82-94.
  4. 澤智裕、熊谷嘉人、赤池孝章. たくさん繋がるS. 実験医学「驚愕の代謝システム」2014; 32: 46-50.
  5. 熊谷嘉人. 活性酸素による化学修飾とセンサータンパク質. 酸化ストレスの医学.2014; 改訂第2版: 154-160.
  6. Yoshida E, Abiko Y, Kumagai Y. Glutathione adduct of methylmercury activates the Keap1-Nrf2 pathway in SH-SY5Y cells. Chemical Research in Toxicology. 2014; 27: 1780-1786.
  7. Kanda H, Shinkai Y, Kumagai Y. S-Mercuration of cellular proteins by methylmercury and its toxicological implications. Journal of Toxicological Sciences. 2014; 39: 687-700.
  8. Contu VR, Kotake Y, Toyama T, Okuda K, Miyara M, Sakamoto S, Samizo S, Sanoh S, Kumagai Y, Ohta S. Endogenous Neurotoxic Dopamine Derivative Covalently Binds to Parkinson's Disease-Associated Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) and Alters Its Structure and Function. Journal of Neurochemistry. 2014; 130: 826-838.
  9. Ida T, Sawa T, Ihara H, Tsuchiya Y, Watanabe Y, Kumagai Y, Suematsu M, Motohashi H, Fujii S, Matsunaga T, Yamamoto M, Ono K, Devarie-Baez NO, Xian M, Fukuto JM, Akaike T. Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling. Proceedings of the National Academy of Sciences, USA 2014; 111: 7606–7611.
  10. Asahi M, Kawai M, Toyama T, Kumagai Y, Chuesaard T, Tang N, Kameda T, Hayakawa K, Toriba A. Identification and quantification of in vivo metabolites of 9,10-phenanthrenequinone in human urine associated with producing reactive oxygen species. Chemical Research in Toxicology 2014; 27: 76-85
  11. Toyama T, Shinkai Y, Yazawa A, Kakehashi H, Kaji T, Kumagai Y. Glutathione-mediated reversibility of covalent modification of ubiquitin carboxyl-terminal hydrolase L1 by 1,2-naphthoquinone through Cys152, but not Lys4. Chemico-Biological Interactions 2014; 214: 41-48.
  12. Shinkai Y, Nakajima S, Eiguren-Fernandez A, Stefano ED, Schmitz DA, Froines JR, Cho AK, Kumagai Y. Ambient vapor samples activate the Nrf2-ARE pathway in human bronchial epithelial BEAS-2B cells. Environmental Toxicology 2014; 29: 1292-1300.


2013

  1. 熊谷嘉人．酸化ストレスと親電子シグナル制御. 医学のあゆみ「活性酸素 – 基礎から病態解明・制御まで」2013; 247: 787-793.
  2. Shinkai Y, Yamanaka I, Duong HHT, Quynh NT, Kanaho Y, Kumagai Y. Garcinia vilersiana bark extract activates the Nrf2/HO-1 signaling pathway in RAW264.7 cells. Journal of Toxicological Sciences 2013: 38: 875-878.
  3. Chang B, Iwamoto N, Inaba T, Shinkai Y, Kumagai Y. Activation of EGFR/MEK/ERK/AP-1 signaling mediated by 1,2-naphthoquinone, an atmospheric electrophile, in human pulmonary A549 cells. Journal of Toxicological Science 2013; 38: 793-797.
  4. Kumagai Y, Kanda H, Shinkai Y, Toyama T. The role of the Keap1/Nrf2 pathway in the cellular response to methylmercury. Oxidative Medicine and Cellular Longevity 2013; Article ID 84827.
  5. Abiko Y, Kumagai Y: Interaction of Keap1 Modified by 2-tert-Butyl-1,4-benzoquinone with GSH: Evidence for S-Transarylation. Chemical Research in Toxicology 2013; 26: 1080–1087.
  6. Shindo R, Kakehashi H, Okumura K, Kumagai Y, Nakano H: Critical contribution of oxidative stress to TNFα-induced necroptosis downstream of RIPK1 activation. Biochemical Biophysical Research Communication 2013; 436: 212-216.
  7. Toyama T, Shinkai Y, Kaji T, Kumagai Y. A convenient method to assess chemical modification of protein thiols by electrophilic metals. Journal of Toxicological Sciences 2013; 38: 477-484.
  8. Koizumi R, Taguchi K, Hisamori M, Kumagai Y. Interaction of 9,10-phenanthraquinone with dithiol causes oxidative modification of Cu,Zn-superoxide dismutase (SOD) through redox cycling. Journal of Toxicological Sciences 2013; 38: 317-324.


2012

  1. 熊谷嘉人. 環境中親電子リガンドの細胞内侵入とそれに伴うシグナル伝達の模倣および攪乱. 実験医学「活性酸素・ガス状分子による恒常性制御と疾患」 2012; 30: 70-75.
  2. Shinkai Y. Kaji T. Cellular defense mechanisms against lead toxicity in the vascular system. Biological Pharmaceutical Bulletin 2012; 35:1885-1891.
  3. Hirose R, Miura T, Sha R, Shinkai Y, Tanaka-Kagawa T, Kumagai Y. A method for detecting covalent modification of sensor proteins associated with 1,4-naphthoquinone-induced activation of electrophilic signal transduction pathways. Journal of Toxicological Sciences 2012; 37: 891-898.
  4. Kanda H, Toyama T, Shinohara-Kanda A, Iwamatsu A, Shinkai Y, Kaji T, Kikushima M, Kumagai Y. S-Mercuration of rat sorbitol dehydrogenase bymethylmercury causes its aggregation and the release of the zinc ion from the active site. Archives of Toxicology 2012; 86: 1693-702.
  5. Nishida M, Sawa T, Kitajima N, Ono K, Inoue H, Ihara H, Motohashi H, Yamamoto M, Suematsu M, Kurose H, van der Vliet A, Freeman BA, Shibata T, Uchida K, Kumagai Y, and Akaike T. Hydrogen sulfide anion regulates redox signaling via electrophile sulfhydration. Nature Chemical Biology 2012; 8: 714–724.
  6. Shinkai Y, Iwamoto N, Miura T, Ishii T, Cho AK, Kumagai Y. Redox cycling of 1,2-naphthoquinone by thioredoxin1 through Cys32 and Cys35 causes inhibition of its catalytic activity and activation of ASK1/p38 signaling. Chemical Research in Toxicology 2012; 25: 1222-1230.
  7. Yamada H, Akahoshi N, Kamata S, Hagiya Y, Hishiki T, Nagahata Y, Matsuura T, Takano N, Mori M, Ishizaki Y, IzumiT, Kumagai Y, Kasahara T, Suematsu M, Ishii I. Methionine excess in diet induces acute lethal hepatitis in mice lacking cystathionine γ -lyase, an animal model of cystathioninuria. Free Radical Biology & Medicine 2012; 52: 1716-1726.
  8. 熊谷嘉人. GAPDHの功罪. 細胞工学「活性酸素シグナル制御とレドックスホメオスタシス」2012; 131: 196-197.
  9. Kumagai Y, Shinkai Y, Miura T, Cho AK. The chemical biology of naphthoquinones and its environmental implications. Annual Review of Pharmacology and Toxicology 2012; 52: 221-247.


2011

  1. Yoshida E, Toyama T, Shinkai Y, Sawa T, Akaike T, Kumagai Y. Detoxification of methylmercury by hydrogen sulfide producing enzyme in mammalian Cells. Chemical Research in Toxicology 2011; 24: 1633-1635.
  2. Takayama N, Iwamoto N, Sumi D, Shinkai Y, Tanaka-Kagawa T, Jinno H, Kumagai Y. Peroxiredoxin 6 is a molecular target for 1,2-naphthoquinone, an atmospheric electrophile, in human pulmonary epithelial A549 cells. Journal of Toxicological Sciences 2011; 36: 817-821.
  3. Miura T, Shinkai Y, Hirose R, Iwamoto N, Cho AK, Kumagai Y. Glyceraldehyde-3-phosphate dehydrogenase as a quinone reductase in the suppression of 1,2-naphthoquinone protein adduct formation. Free Radical Biology & Medicine 2011; 51: 2082-2089.
  4. Toyama T, Yoshida E, Shinkai Y, Kumagai Y. DNA microarray analysis of human neuroblastoma SH-SY5Y cells exposed to methylmercury. Journal of Toxicological Sciences 2011; 36: 843-845.
  5. Miura T, Kakehashi H, Shinkai Y, Egara Y, Hirose R, Cho AK, Kumagai Y. GSH-mediated S-transarylation of a quinone glyceraldehyde-3-phosphate dehydrogenase conjugate. Chemical Research in Toxicology 2011; 24: 1836-1844.
  6. Abiko Y, Miura T, Bui H Phuc, Shinkai Y, Kumagai Y. Participation of covalent modification of Keap1 in the activation of Nrf2 by tert-butylbenzoquinone, an electrophilic metabolite of butylated hydroxyanisole. Toxicology and Applied Pharmacology 2011; 255: 32-39.
  7. Endo A, Sumi D, Iwamoto N, Kumagai Y. Inhibition of DNA binding activity of cAMP response element–binding protein by 1,2-naphthoquinone through chemical modification of Cys-286. Chemico-Biological Interactions 2011; 192: 272-277.
  8. Toyama T, Shinkai Y, Yasutake A, Uchida K, Yamamoto M, Kumagai Y. Isothiocyanates reduce mercury accumulation via an Nrf2-dependent mechanism during exposure of mice to methylmercury. Environmental Health Perspectives 2011; 119: 1117-1122.
  9. Miura T, Shinkai Y, Jiang HY, Iwamoto N, Sumi D, Taguchi K, Yamamoto M, Jinno H, Tanaka-Kagawa T, Cho AK, Kumagai Y. Initial response and cellular protection through the Keap1/Nrf2 system during exposure of primary mouse hepatocytes to 1,2-naphthoquinone. Chemical Research in Toxicology 2011; 24: 559-567.
  10. Fujiwara Y, Banno H, Shinkai Y, Yamamoto C, Kaji T, Satoh M. Protective effect of pretreatment with cilostazol on cytotoxicity of cadmium and arsenite in cultured vascular endothelial cells. Journal of Toxicological Sciences 2011; 36: 155-161.
  11. Yamano S, Shibata M, Kita H, Matsusue K, Narimatsu S, Taguchi K, Kumagai Y. Two-electron quinone reductase (AKR1C isozyme) augments the oxidative DNA damage induced by quinones in diesel exhaust particles by accelerating redox cycling. Journal of Health Science 2011; 57: 107-114.
  12. Tsujita T, Li L, Nakajima H, Iwamoto N, Nakajima-Takagi Y, Ohashi K, Kawakami K, Kumagai Y, Freeman BA, Yamamoto M, Kobayashi M. Nitro-fatty acids and cyclopentenone prostaglandins share strategies to activate the Keap1-Nrf2 system: a study using GFP transgenic zebrafish.Genes to Cells 2011; 16: 46-57.

（2006-2010）

RETURN TO TOP