業 績
(２０１4)
Ueshima S, Nagata K, Okuwaki M.
Upstream binding factor-dependent and pre-rRNA transcription-independent association of pre-rRNA processing factors with rRNA gene. Biochem Biophys Res Commun. 2014 Jan 3;443(1):22-7. Pub Med
Hisaoka M, Nagata K, Okuwaki M.
Intrinsically disordered regions of nucleophosmin/B23 regulate its RNA binding activity through their inter- and intra-molecular association. Nucleic Acids Res. 2014 Jan;42(2):1180-95. Pub Med
(２０１２)
Okuwaki M., Sumi A., Hisaoka M., Saotome-Nakamura A., Akashi S., Nishimura Y., Nagata K. Function of homo- and hetero-oligomers of human nucleoplasmin/nucleophosmin family proteins NPM1, NPM2 and NPM3 during sperm chromatin remodeling. Nucleic Acids Res. 2012 40 (11): 4861-78 Pub Med
(２０１１）
Kato K., Okuwaki M., Nagata K. Role of Template Activating Factor-I as a chaperone in linker histone dynamics. J. Cell Sci. 2011 124: 3254-65 PubMed
(２０１０）
Hisaoka M, Ueshima S, Murano K, Nagata K, and Okuwaki M. Regulation of nucleolar chromatin by B23/nucleophosmin jointly depends upon its RNA binding activity and transcription factor UBF. Mol Cell Biol. 2010 30(20):4952-62 PubMed
Okuwaki M, Kato K, Nagata K. Functional characterization of human nucleosome assembly protein 1-like proteins as histone chaperones. Genes Cells. 2010 Jan 15(1):13-27 PubMed
(２００８)
Komaki-Yasuda K, Okuwaki M, Kano S, Nagata K, Kawazu S. 5' sequence- and chromatin modification-dependent gene expression in Plasmodium falciparum erythrocytic stage. Mol Biochem Parasitol. 2008 Nov;162(1):40-51. Epub 2008 Jul 23. PubMed
Murano K., Okuwaki, M., Hisaoka, M., and Nagata, K. Transcription regulation of rRNA gene by a multi-functional nucleolar protein, B23/nucleophosmin through its histone chaperone activity. Mol Cell Biol. 2008 8(10):3114-26 PubMed
Murayama A, Ohmori K, Fujimura A, Minami H, Yasuzawa-Tanaka K, Kuroda T, Oie S, Daitoku H, Okuwaki M, Nagata K, Fukamizu A, Kimura K, Shimizu T, Yanagisawa J. Epigenetic control of rDNA loci in response to intracellular energy status. Cell. 2008 133(4):627-39. PubMed
(２００7)
Samad MA., Okuwaki, M., Haruki, H., and Nagata, K. Physical and functional interaction between a nucleolar protein nucleophosmin/B23 and adenovirus basic core proteins. FEBS Lett. 2007; 581(17):3283-8 PubMed
Kato K, Miyaji-Yamaguchi M, Okuwaki M, Nagata K. Histone acetylation-independent transcription stimulation by a histone chaperone.Nucleic Acids Res. 2007;35(3):705-15. PubMed
(２００6)
Haruki H, Okuwaki M, Miyagishi M, Taira K, Nagata K. Involvement of template-activating factor I/SET in transcription of adenovirus early genes as a positive-acting factor.J Virol. 2006 Jan;80(2):794-801. PubMed
(２００5)
Okuwaki M, Kato K, Shimahara H, Tate S, Nagata K. Assembly and disassembly of nucleosome core particles containing histone variants by human nucleosome assembly protein I. Mol Cell Biol. 2005 Dec;25(23):10639-51. PubMed
(２００4)
Thiru A, Nietlispach D, Mott HR, Okuwaki M, Lyon D, Nielsen PR, Hirshberg M, Verreault A, Murzina NV, Laue ED. Structural basis of HP1/PXVXL motif peptide interactions and HP1 localisation to heterochromatin.EMBO J. 2004 Feb 11;23(3):489-99. PubMed
Okuwaki M, Verreault A. Maintenance DNA methylation of nucleosome core particles.J Biol Chem. 2004 Jan 23;279(4):2904-12. PubMed
(~２００3)
Haruki H, Gyurcsik B, Okuwaki M, Nagata K. Ternary complex formation between DNA-adenovirus core protein VII and TAF-Ibeta/SET, an acidic molecular chaperone.FEBS Lett. 2003 Dec 18;555(3):521-7. PubMed
van Leeuwen H, Okuwaki M, Hong R, Chakravarti D, Nagata K, O'Hare P. Herpes simplex virus type 1 tegument protein VP22 interacts with TAF-I proteins and inhibits nucleosome assembly but not regulation of histone acetylation by INHAT.J Gen Virol. 2003 Sep;84(Pt 9):2501-10. PubMed
Okuwaki M, Tsujimoto M, Nagata K. The RNA binding activity of a ribosome biogenesis factor, nucleophosmin/B23, is modulated by phosphorylation with a cell cycle-dependent kinase and by association with its subtype. Mol Biol Cell. 2002 Jun;13(6):2016-30. PubMed
Okuwaki M, Matsumoto K, Tsujimoto M, Nagata K. Function of nucleophosmin/B23, a nucleolar acidic protein, as a histone chaperone.FEBS Lett. 2001 Oct 12;506(3):272-6. PubMed
Okuwaki M, Iwamatsu A, Tsujimoto M, Nagata K. Identification of nucleophosmin/B23, an acidic nucleolar protein, as a stimulatory factor for in vitro replication of adenovirus DNA complexed with viral basic core proteins.J Mol Biol. 2001 Aug 3;311(1):41-55. PubMed
Matsumoto K, Nagata K, Okuwaki M, Tsujimoto M. Histone- and chromatin-binding activity of template activating factor-I.FEBS Lett. 1999 Dec 17;463(3):285-8. PubMed
Miyaji-Yamaguchi M, Okuwaki M, Nagata K. Coiled-coil structure-mediated dimerization of template activating factor-I is critical for its chromatin remodeling activity.J Mol Biol. 1999 Jul 9;290(2):547-57. PubMed
Okuwaki M, Nagata K. Template activating factor-I remodels the chromatin structure and stimulates transcription from the chromatin template.J Biol Chem. 1998 Dec 18;273(51):34511-8. PubMed
Nagata K, Saito S, Okuwaki M, Kawase H, Furuya A, Kusano A, Hanai N, Okuda A, Kikuchi A. Cellular localization and expression of template-activating factor I in different cell types.Exp Cell Res. 1998 May 1;240(2):274-81. PubMed
Kawase H, Okuwaki M, Miyaji M, Ohba R, Handa H, Ishimi Y, Fujii-Nakata T, Kikuchi A, Nagata K. NAP-I is a functional homologue of TAF-I that is required for replication and transcription of the adenovirus genome in a chromatin-like structure.Genes Cells. 1996 Dec;1(12):1045-56. PubMed
Matsumoto K, Okuwaki M, Kawase H, Handa H, Hanaoka F, Nagata K. Stimulation of DNA transcription by the replication factor from the adenovirus genome in a chromatin-like structure.J Biol Chem. 1995 Apr 21;270(16):9645-50 PubMed
Review etc.
永田恭介、奥脇暢
「目的別で選べるタンパク質発現プロトコール」実験医学別冊
（羊土社）(2010)
Okuwaki M. The Structure and Functions of NPM1/Nucleophsmin/B23, a Multifunctional Nucleolar Acidic Protein. J Biochem. 2008 Apr;143(4):441-8. PubMed
奥脇暢、小松哲郎、永田恭介 ヒストンシャペロン 五十嵐和彦、深水昭吉、大熊芳明、山本雅之編 転写因子による生命現象解明の最前線 実験医学増刊 2007；36-45
奥脇暢 核小体の構造と機能 米田悦啓、木村宏、五十嵐和彦、中尾光喜編 蛋白質・核酸・酵素 増刊「細胞核の世界」2006；1950－1956
Mitsuru Okuwaki, Kensaku Murano, Kohsuke Kato, and Kyosuke Nagata Molecular and physiological roles of histone chaperones DNA Structure, Chromatin, and Gene Expression Edited by Ryoiti Kiyama and Mitsuhiro Shimizu 2006；121-144
奥脇暢、永田恭介 クロマチンの制御 田村隆明編 キーワードで理解する転写イラストマップ 2004；59-70
奥脇暢、永田恭介 クロマチンのリモデリングと動的変化 竹安邦夫、米田悦啓編 細胞核のダイナミクス, シュプリンガーフェアラーク東京 2004；63-78
永田恭介、齋藤祥子、奥脇暢. クロマチン制御因子と癌化. 遺伝子医学 1999; 3: 659-666
Nagata K, Miyaji M, Okuwaki M, Saito S. Function of TAF-I as an adenoviral chromatin remodeling factor. From viruses to cell cycle regulation through oncogene 1997; 69-75
永田恭介、奥脇暢. アデノウイルスクロマチンの複製とクロマチンリモデリング. 永井美之、野本明男、山本直樹編. ウイルス最前線. 東京: 羊土社, 1997; 193-200
奥脇暢、永田恭介. DNAトランスアクション-転写反応を中心に-. 田村隆明、村松正美編. 転写のメカニズムと疾患. 東京: 羊土社, 1997; 44-50
