CRISPR/Cas9を用いて作製した遺伝子改変マウス論文 　　

2024.2.29 up to now

• Jo-Watanabe A, Inaba T, Osada T, Hashimoto R, Nishizawa T, Okuno T, Ihara S, Touhara K, Hattori N, Oh-Hora M, Nurek O, Yokomizo T. Bicarbonate signalling via G protein-coupled receptor regulates ischaemia-reperfusion injury. Nat Commun. 2024 Feb 27;15(1):1530. doi: 10.1038/s41467-024-45579-3. 詳細

• Tamura R, Sabu Y, Mizuno T, Mizuno S, Nakano S, Suzuki M, Abukawa D, Kaji S, Azuma Y, Inui A, Okamoto T, Shimizu S, Fukuda A, Sakamoto S, Kasahara M, Takahashi S, Kusuhara H, Zen Y, Ando T, Hayashi H. Intestinal Atp8b1 dysfunction causes hepatic choline deficiency and steatohepatitis. Nat Commun. 2023 Nov 21;14(1):6763. doi: 10.1038/s41467-023-42424-x. 詳細

• Fujisawa M, Nguyen TB, Abe Y, Suehara Y, Fukumoto K, Suma S, Makishima K, Kaneko C, Nguyen YTM, Usuki K, Narita K, Matsue K, Nakamura N, Ishikawa S, Miura F, Ito T, Suzuki A, Suzuki Y, Mizuno S, Takahashi S, Chiba S, Sakata-Yanagimoto M. Clonal germinal center B cells function as a niche for T-cell lymphoma. Blood. 2022 Aug 3:blood.2022015451. doi: 10.1182/blood.2022015451.　詳細

• Kim P, Park J, Lee DJ, Mizuno S, Shinohara M, Hong CP, Jeong Y, Yun R, Park H, Park S, Yang KM, Lee MJ, Jang SP, Kim HY, Lee SJ, Song SU, Park KS, Tanaka M, Ohshima H, Cho JW, Sugiyama F, Takahashi S, Jung HS, Kim SJ. Mast4 determines the cell fate of MSCs for bone and cartilage development. Nat Commun. 2022 Jul 8;13(1):3960. doi: 10.1038/s41467-022-31697-3. 詳細

• Ojima K, Kakegawa W, Yamasaki T, Miura Y, Itoh M, Michibata Y, Kubota R, Doura T, Miura E, Nonaka H, Mizuno S, Takahashi S, Yuzaki M, Hamachi I, Kiyonaka S. Coordination chemogenetics for activation of GPCR-type glutamate receptors in brain tissue. Nat Commun. 2022 Jun 16;13(1):3167. doi: 10.1038/s41467-022-30828-0. 詳細

• Nakagawa T, Jörg DJ, Watanabe H, Mizuno S, Han S, Ikeda T, Omatsu Y, Nishimura K, Fujita M, Takahashi S, Kondoh G, Simons BD, Yoshida S, Nagasawa T. A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis. Cell Rep. 2021 Oct 19;37(3):109875. doi: 10.1016/j.celrep.2021.109875. 詳細

• Ishii S, Suzuki T, Wakahashi K, Asada N, Kawano Y, Kawano H, Sada A, Minagawa K, Nakamura Y, Mizuno S, Takahashi S, Matsui T, Katayama Y. FGF23 from erythroblasts promotes hematopoietic progenitor mobilization. Blood. 2021 Mar 18;137(11):1457-1467. doi: 10.1182/blood.2020007172. 詳細

• Lee SJ, Park J, Lee DJ, Otsu K, Kim P, Mizuno S, Lee MJ, Kim HY, Harada H, Takahashi S, Kim SJ, Jung HS. Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway. Cell Death Differ.  2021 May;28(5):1441-1454. doi: 10.1038/s41418-020-00670-2. Epub 2020 Nov 20. 詳細

• Matsuzaka T, Kuba M, Koyasu S, Yamamoto Y, Motomura K, Arulmozhiraja S, Ohno H, Sharma R, Shimura T, Okajima Y, Han SI, Aita Y, Mizunoe Y, Osaki Y, Iwasaki H, Yatoh S, Suzuki H, Sone H, Takeuchi Y, Yahagi N, Miyamoto T, Sekiya M, Nakagawa Y, Ema M, Takahashi S, Tokiwa H, Shimano H. Hepatocyte ELOVL Fatty Acid Elongase 6 Determines Ceramide Acyl-Chain Length and Hepatic Insulin Sensitivity in Mice.
  Hepatology. 2020 May;71(5):1609-1625. doi: 10.1002/hep.30953. Epub 2020 Feb 7. 詳細

• Liu M, Tong Z, Ding C, Luo F, Wu S, Wu C, Albeituni S, He L, Hu X, Tieri D, Rouchka EC, Hamada M, Takahashi S, Gibb AA, Kloecker G, Zhang HG, Bousamra M 2nd, Hill BG, Zhang X, Yan J. Transcription factor c-Maf is a checkpoint that programs macrophages in lung cancer.
  J Clin Invest. 2020 Apr 1;130(4):2081-2096. doi: 10.1172/JCI131335. 詳細

• Kanemaru K, Noguchi E, Tahara-Hanaoka S, Mizuno S, Tateno H, Denda-Nagai K, Irimura T, Matsuda H, Sugiyama F, Takahashi S, Shibuya K, Shibuya A. Clec10a regulates mite-induced dermatitis.
  Sci Immunol. 2019 Dec 6;4(42):eaax6908. doi: 10.1126/sciimmunol.aax6908. 詳細

• Gurumurthy CB, O'Brien AR, Quadros RM, Adams J Jr, Alcaide P, Ayabe S, Ballard J, Batra SK, Beauchamp MC, Becker KA, Bernas G, Brough D, Carrillo-Salinas F, Chan W, Chen H, Dawson R, DeMambro V, D'Hont J, Dibb KM, Eudy JD, Gan L, Gao J, Gonzales A, Guntur AR, Guo H, Harms DW, Harrington A, Hentges KE, Humphreys N, Imai S, Ishii H, Iwama M, Jonasch E, Karolak M, Keavney B, Khin NC, Konno M, Kotani Y, Kunihiro Y, Lakshmanan I, Larochelle C, Lawrence CB, Li L, Lindner V, Liu XD, Lopez-Castejon G, Loudon A, Lowe J, Jerome-Majewska LA, Matsusaka T, Miura H, Miyasaka Y, Morpurgo B, Motyl K, Nabeshima YI, Nakade K, Nakashiba T, Nakashima K, Obata Y, Ogiwara S, Ouellet M, Oxburgh L, Piltz S, Pinz I, Ponnusamy MP, Ray D, Redder RJ, Rosen CJ, Ross N, Ruhe MT, Ryzhova L, Salvador AM, Alam SS, Sedlacek R, Sharma K, Smith C, Staes K, Starrs L, Sugiyama F, Takahashi S, Tanaka T, Trafford AW, Uno Y, Vanhoutte L, Vanrockeghem F, Willis BJ, Wright CS, Yamauchi Y, Yi X, Yoshimi K, Zhang X, Zhang Y, Ohtsuka M, Das S, Garry DJ, Hochepied T, Thomas P, Parker-Thornburg J, Adamson AD, Yoshiki A, Schmouth JF, Golovko A, Thompson WR, Lloyd KCK, Wood JA, Cowan M, Mashimo T, Mizuno S, Zhu H, Kasparek P, Liaw L, Miano JM, Burgio G. Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation. Genome Biol. 2019 Aug 26;20(1):171. doi: 10.1186/s13059-019-1776-2. 詳細

• Yasuda D, Kobayashi D, Akahoshi N, Ohto-Nakanishi T, Yoshioka K, Takuwa Y, Mizuno S, Takahashi S, Ishii S. Lysophosphatidic acid-induced YAP/TAZ activation promotes developmental angiogenesis by repressing Notch ligand Dll4.
  J Clin Invest. 2019 Jul 23;130. pii: 121955. doi: 10.1172/JCI121955. 詳細

• Miyagi S, Sroczynska P, Kato Y, Nakajima-Takagi Y, Oshima M, Rizq O, Takayama N, Saraya A, Mizuno S, Sugiyama F, Takahashi S, Matsuzaki Y, Christensen J, Helin K, Iwama A. The chromatin binding protein Phf6 restricts the self-renewal of hematopoietic stem cells.
  Blood. 2019 Jun 6;133(23):2495-2506. doi: 10.1182/blood.2019000468. Epub 2019 Mar 27. 詳細

• Honda T, Fujiyama T, Miyoshi C, Ikkyu A, Hotta-Hirashima N, Kanno S, Mizuno S, Sugiyama F, Takahashi S, Funato H, Yanagisawa M. A single phosphorylation site of SIK3 regulates daily sleep amounts and sleep need in mice.
  Proc Natl Acad Sci USA. 2018 Oct 9;115(41):10458-10463. doi: 10.1073/pnas.1810823115. 詳細

• Mali GR, Yeyati PL, Mizuno S, Dodd DO, Tennant PA, Keighren MA, Zur Lage P, Shoemark A, Garcia-Munoz A, Shimada A, Takeda H, Edlich F, Takahashi S, von Kreisheim A, Jarman AP, Mill P. ZMYND10 functions in a chaperone relay during axonemal dynein assembly.
  Elife. 2018 Jun 19;7. pii: e34389. doi: 10.7554/eLife.34389. 詳細

• Wang Z, Ma J, Miyoshi C, Li Y, Sato M, Ogawa Y, Lou T, Ma C, Gao X, Lee C, Fujiyama T, Yang X, Zhou S, Hotta-Hirashima N, Klewe-Nebenius D, Ikkyu A, Kakizaki M, Kanno S, Cao L, Takahashi S, Peng J, Yu Y, Funato H, Yanagisawa M, Liu Q . Quantitative phosphoproteomic analysis of the molecular substrates of sleep need.
  Nature. 2018 Jun;558(7710):435-439. doi: 10.1038/s41586-018-0218-8. Epub 2018 Jun 13. 詳細

• Hoshino Y, Mizuno S, Kato K, Mizuno-Iijima S, Tanimoto Y, Ishida M, Kajiwara N, Sakasai T, Miwa Y, Takahashi S, Yagami KI, Sugiyama F. Simple generation of hairless mice for in vivo imaging.
  Exp Anim.2017 Oct 30;66(4):437-445. doi: 10.1538/expanim.17-0049. Epub 2017 Jul 18.詳細

• Tokue M, Ikami K, Mizuno S, Takagi C, M Asuka, Takada R, Noda C, Kitadate Y, Hara K, Mizuguchi H, Sato T, Mark Taketo M, Sugiyama F, Ogawa T, Kobayashi S, Ueno N, Takahashi S, Takada S, Yoshida S. SHISA6 confers resistance to differentiation-promoting Wnt/β-catenin signaling in mouse spermatogenic stem cells.
  Stem Cell Reports,doi.org/10.1016/j.stemcr. 2017.01.006. 詳細

• Nakagawa Y, Oikawa F, Mizuno S, Ohno H, Yagishita Y, Satoh A, Osaki Y, Takei K, Kikuchi T, Han SI, Matsuzaka T, Iwasaki H, Kobayashi K, Yatoh S, Yahagi N, Isaka M, Suzuki H, Sone H, Takahashi S, Yamada N, Shimano H. Hyperlipidemia and hepatitis in liver-specific CREB3L3 knockout mice generated using a one-step CRISPR/Cas9 system.
  Sci Rep. 2016 Jun 13;6:27857. doi: 10.1038/srep27857.（floxマウスの作製）詳細

• Kataoka K, Shiraishi Y, Takeda Y, Sakata S, Matsumoto M, Nagano S, Maeda T, Nagata Y, Kitanaka A, Mizuno S, Tanaka H, Chiba K, Ito S, Watatani Y, Kakiuchi N, Suzuki H, Yoshizato T, Yoshida K, Sanada M, Itonaga H, Imaizumi Y, Totoki Y, Munakata, W, Nakamura H, Hama N, Shide K, Kubuki Y, Hidaka T, Kameda T, Masuda K, Minato N, Kashiwase K, Izutsu K, Takaori-Kondo A, Miyazak Y, Takahashi S, Shibata T, Kawamoto H, Akatsuka Y, Shimoda K, Takeuchi K, Seya T, Miyano S & Ogawa S. Aberrant PD-L1 expression through 3′-UTR disruption in multiple cancers. Nature. 2016 May 23; 534(7607): 402-6. 詳細

• Hasegawa Y, Hoshino Y, Ibrahim AE, Kato K, Daitoku Y, Tanimoto Y, Ikeda Y, 7 Yoshiki A, Yagami K-i, Iseki H, Mizuno S, Sugiyama F. Generation of CRISPR/Cas9-mediated bicistronic knock-in Ins1-cre driver mice. Exp. Animal. 2016 Jul 29;65(3):319-27. doi: 10.1538/expanim.16-0016. Epub 2016 Apr 7 （knock-inマウスの作製）詳細

• Ohmura S, Mizuno S, Oishi H, Ku C-J, Clifford M, Hosoya T, Takahashi S, Engel J D. The Gata3 TCE1 enhancer mediates T cell-specific activation through lineage affiliated transcription factors TCF-1, HEB and RBPJ.
  J Clin Invest. 2016. Mar.1:126(3):865-878. doi: 10.1172（ゲノム上からのGATA-3遺伝子制御領域を欠失させた論文） 詳細

• Mizuno S, Takami K, Daitoku Y, Tanimoto Y, Dinh T, Mizuno-Iijima S, Hasegawa Y, Takahashi S, Sugiyama F, Yagami K-i. Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation. Sci Rep. 2015 Sep 8;5:13632. doi: 10.1038（Mb単位のゲノム欠損作製論文） 詳細

• Mizuno S, Dinh TT, Kato K, Mizuno-Iijima S, Tanimoto Y, Daitoku Y, Hoshino Y, Ikawa M, Takahashi S, Sugiyama F, Yagami KI. Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system.
  Mamm Genome. 25, 327-334, 2014.（nullおよび点変異導入論文） 詳細