Laboratory Animal Research Core 

Professor: Fumihiro Sugiyama

Research Associate: Seiya Mizuno

Specially Appointed Professor: Kenichi Yagami

Laboratory animals are broadly utilized as genetic resources in medical and life science researches. Their characteristics are genetically proved and are extensively controlled in their microbiological quality. In Laboratory Animal Research Core, we are committed to analyzing mutant/ genetically-modified mouse, studying microbial infection of laboratory animals, creating disease-model mice by genetic modification, and establishment of embryonic stem cells and consomic mouse strains. We also engage in health control of laboratory animals as well as in education and technical guidance of laboratory animal science and animal experimentation.

● Research on infection of mice and rats

1) Development of high throughput diagnostics method for infectious diseases in mice and rats

2) Molecular mechanism of geneticmodiification in host cells by rodent parvoviruses

3) Virulence mechanism and diagnostics of Helicobacter infection in mice

● Genetic analysis of mouse

1) Searching hypertension・obesity・diabetes - related genes

2) Searching genes responsible for mutant mice

● Development of biological resources

1) Establishing new embryo- stem cell line derived from inbred mice

2) Development of metabolic syndrome consomic mouse strains

Phenotypic changes of parvovius-infected tumor Cells
Parvovirus NS gene indused phenotype changes such as enhanced cell adherence (a and b) and suppressed tumorigenicity (c and d) in thymic lymphpma cell line.
Genetic analysis of mutant mice
a) Fig5 moutant mouse (Moja) with long Pelage hair.
b) C57BL/6J mouse with congenital white spoting.
c) NZO mouse with metabolic syndrome.
d) Responsible gene for agenesis oF corpus callosum in TAS mouse,
Reserch on infectious diseases in mice and rats
a) Cell distension caused by Helicobactor hepaticus virulence factor CDT.
b) Hepatic tumor formation in mice infected with Helicobactor hepatics.
Development of bio-resources
a) Chimeric mice deliverd from C57BL/6 ES cells expressing EGFP.
b)Fetus showed ubiquitous green fluorescence.

1)Sakairi A, et al (2008) Angiotensin type 1 receptor blockade prevents cardiac remodeling in mice with pregnancy-associated hypertension. Hypertens Res. 31: 2165-2175
2)Tanimoto Y, et al (2008) Embryonic stem cells derived from C57BL/6J and C57BL/6N mice. Comp Med. 58: 347-352
3)Nishihara E, et al (2007) Quantitative trait loci associated with blood pressure of metabolic syndrome in the progeny of NZO/HILtJxC3H/HeJ intercrosses. Mamm Genome. 18: 573-583
4) Kunita S, et al (2006) Development of ELISA using recombinant antigens for specific detection of mouse parvovirus infection. Exp Anim. 55:117-124
5) Shimizukawa R, et al (2005) Establishment of a new embryonic stem cell line derived from C57BL/6 mouse expressing EGFP ubiquitously. Genesis. 42: 47-52.
6) Iseki H, et al (2005) Parvovirus nonstructural proteins induce an epigenetic modification through histone acetylation in host genes and revert tumor malignancy to benignancy. J Virol. 79: 8886-8893