Development of technology to amplify hematopoietic stem cells in vitro and enable free gene transfer
(Note: Text has been auto translated from Japanese language.)
Hematopoietic stem cells have the ability to differentiate into various blood cells such as red blood cells, white blood cells, and platelets, and are being applied to the treatment of blood cancer and hereditary blood diseases. However, hematopoietic stem cells are rarely present in the bone marrow, and post-collection amplification is essential.
As a technique for mass amplification of hematopoietic stem cells derived from donor mice in vitro, a method using a medium supplemented with polyvinyl alcohol (PVA) has been reported. Therefore, this research team applied this method to maintain the function of hematopoietic stem cells from bone marrow cells containing a very small amount of hematopoietic stem cells by only immunomagnetic cell separation method using magnetic beads and subsequent simple culture operation. We have developed a technology that can be selectively purified and concentrated as it is. This facilitates gene transfer into hematopoietic stem cells outside the body, and at the same time, it can be amplified in large quantities, making it possible to transplant without using conventional pretreatment such as irradiation. Furthermore, by devising when introducing the target gene, by selecting and transplanting only the hematopoietic stem cells into which the gene has been correctly introduced, the target gene can be expressed in the blood cells in the mouse body at any time after transplantation. Was successful. With this method, there is no tissue damage caused by radiation to the recipient mice after transplantation, and stable breeding and observation can be performed for a long period of time. In addition, there is little invasion and tissue damage to living mice, and it is considered to be an excellent method from the viewpoint of animal welfare. By using these platforms, it has become possible to freely design the function of hematopoietic stem cells according to the purpose of treatment.
12, Article number: 3568 (2021)
Non-conditioned bone marrow chimeric mouse generation using culture based enrichment
of hematopoietic stem and progenitor cells.
→ Research Outline (PDF In Japanese language)