Genetically Modified Mice (GMM) provide understanding of in vivo gene function. The Laboratory Animal Resource Center (LARC) to which our
lab belong is one of the biggest GMM production institutes in Japan. We
produce around 100 novel GMM strains per year by precision embryo and stem cell manipulation skill and newest gene modification technique.
Since 2013, we started GMM production with CRISPR/Cas9 system. With this system, we have produced many genome edited mice carried precise point mutation, large deletion, fluorescence gene Knock-in, and genetic humanized alleles.
The conditional Knock-out (cKO) technique enable tissue and/or time specific
gene deletion in vivo. The Cre-LoxP is the most commonly used system for cKO experiments. The
gene region between two inserted loxP sites, called flox region, is looped
out from the chromosome by Cre enzyme dependent manner. So, it is very
important to recognize where and when Cre recombination happen in each
Cre-driver mouse strain.
We developed a new Cre-Reporter mouse and named it GRR mouse. The GRR mouse greportsh the Cre recombination in vivo by two different fluorescence colors. With this mouse, we are developing the Cre recombination atlas.
Furthermore, we are now developing the bicistronic Cre driver mouse using with CRISPR/Cas9 system.
related papers1, 2, 3
Tennodai 1-1-1,Tsukuba, Ibaraki,
Laboratory Animal Resource Center
B building, 2nd floor