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〒305-8575
茨城県つくば市天王台 1-1-1
筑波大学医学医療系
遺伝子制御学グループ
健康医科学イノベーション棟4階(402-3)
Tel/Fax: 029-853-3929

研究業績

原著論文

  • Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M: Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302. Stem Cell Research, 23:13-19(2017)
  • Nishimura K, Aizawa S, Nugroho FL, Shiomitsu E, Tran YT, Bui PL, Borisova E, Sakuragi Y, Takada H, Kurisaki A, Hayashi Y, Fukuda A, Nakanishi M, Hisatake K: A Role for KLF4 in Promoting the Metabolic Shift via TCL1 during Induced Pluripotent Stem Cell Generation. Stem Cell Reports 8(3):787-801(2017)
  • Hayashi Y, Hsiao EC, Sami S, Lancero M, Schlieve CR, Nguyen T, Yano K, Nagahashi A, Ikeya M, Matsumoto Y, Nishimura K, Fukuda A, Hisatake K, Tomoda K, Asaka I, Toguchida J, Conklin BR, and Yamanaka S: BMP-SMAD-ID Promotes Reprogramming to Pluripotency by Inhibiting p16/INK4A-Dependent Senescence. Proc. Natl. Acad. Sci. USA 113(46):13057-13062(2016)
  • Kime C, Sakaki-Yumoto, Goodrich L, Hayashi Y, Sami S, Derynck R, Asahi M, Panning B, Yamanaka S, and Tomoda K: Autotaxin-mediated Lipid Signaling Intersects with LIF and BMP Signaling to Promote the Naïve Pluripotency Transcription Factor Program. Proc. Natl. Acad. Sci. USA (in press)(2016)
  • Suga M, Hayashi Y, Furue MK: In Vitro Models of Cranial Neural Crest Development toward Toxicity Tests: Frog, Mouse and HumanOral Diseases (in press)(2016)
  • Hayashi Y and Furue MK: Biological Effects of Culture Substrates on Human Pluripotent Stem Cells.Stem Cells Int. 2016: 5380560 (2016)
  • Banno K, Omori S, Hirata K, Nawa N, Nakagawa N, Nishimura K, Ohtaka M, Nakanishi M, Sakuma T, Yamamoto T, Toki T, Ito E, Yamamoto T, Kokubu C, Takeda J, Taniguchi H, Arahori H, Wada K, Kitabatake Y, Ozono K: Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities.Cell Reports Vol. 15(6), 1228-1241, doi: 10.1016/j.celrep.2016.04.031.(2016)
  • Bueno C, Sardina JL, Di Stefano B, Romero-Moya D, Muñoz-López A, Ariza L, Chillón MC, Balanzategui A, Castaño J, Herreros A, Fraga MF, Fernández A, Granada I, Quintana-Bustamante O, Segovia JC, Nishimura K, Ohtaka M, Nakanishi M, Graf T, Menendez P: Reprogramming human B cells into induced pluripotent stem cells and its enhancement by C/EBPα.Leukemia Vol. 30(3), 674-682,doi:10.1038/leu.2015.294.(2016)
  • Okamura K, Sakaguchi H, Sakamoto-Abutani R, Nakanishi M, Nishimura K, Yamazaki-Inoue M, Ohtaka M, Periasamy VS, Alshatwi AA, Higuchi A, Hanaoka K, Nakabayashi K, Takada S, Hata K, Toyoda M, Umezawa A: Distinctive features of single nucleotide alterations in induced pluripotent stem cells with different types of DNA repair deficiency disorders.Scientific Reports Vol. 6, 26342,doi:10.1038/srep26342 (2016)
  • Matsumoto T, Fujimori K, Andoh-Noda T, Ando T, Kuzumaki N, Toyoshima M, Tada H, Imaizumi K, Ishikawa M, Yamaguchi R, Isoda M, Zhou Z, Sato S, Kobayashi T, Ohtaka M, Nishimura K, Kurosawa H, Yoshikawa T, Takahashi T, Nakanishi M, Ohyama M, Hattori N, Akamatsu W, Okano H: Functional neurons generated from T cell-derived iPSCs for neurological disease modeling. Stem Cell ReportsVol. 6(3), 422-435 (2016)
  • Kyttälä A, Moraghebi R, Valensisi C, Kettunen J, Andrus C, Pasumarthy KK, Nakanishi M, Nishimura K, Ohtaka M, Weltner J, Handel BV, Parkkonen O, Sinisalo J, Jalanko A, Hawkins RD, Woods NB, Otonkoski T, Trokovic R: Genetic variability overrides the impact of parental cell-type and determines iPSCs differentiation potential. Stem Cell ReportsVol. 6(2), 200-212 (2016)
  • Yamasaki S, Hamada A, Akagi E, Nakatao H, Ohtaka M, Nishimura K, Nakanishi M, Toratani S, Okamoto T: Generation of cleidocranial dysplasia-specific human induced pluripotent stem cells in completely serum-, feeder-, and integration-free culture. In Vitro Cell. Dev. Biol.-AnimalVol. 52, 252-264 (2016)
  • Lin HT, Masaki H, Yamaguchi T, Wada T, Yachie A, Nishimura K, Ohtaka M, Nakanishi M, Nakauchi H, Otsu M: An assessment of the effects of ectopic gp91phox expression in XCGD iPSC-derived neutrophils. Mol. Ther. Methods Clin. Dev.Vol. 2, 15046 (2015)
  • Nakadai T, Fukuda A, Shimada M, Nishimura K, Hisatake K: The RNA-binding Complexes, NF45-NF90 and NF45-NF110, Associate Dynamically with the c-fos Gene and Function as Transcriptional Coactivators. J. Biol. Chem. pii: jbc.M115.688317. (2015)
  • Ando M, Nishimura T, Yamazaki S, Kawana-Tachikawa A, Hayama T, Nakauchi Y, Ando J, Ota Y, Takahashi S, Nishimura K, Ohtaka M, Nakanishi M, Miles JJ, Burrows SR, Brenner MK, Nakauchi H: A Safeguard System for Induced Pluripotent Stem Cell-Derived Rejuvenated T Cell Therapy. Stem Cell Reports pii: S2213-6711(15)00217-9. doi: 10.1016/j.stemcr.2015.07.011 (2015)
  • Tanaka A, Akimoto Y, Kobayashi S, Hisatake K, Hanaoka F, Ohkuma Y: Association of the winged helix motif of the TFIIEα subunit of TFIIE with either the TFIIEβ subunit or TFIIB distinguishes its functions in transcription. Genes Cells 20(3), 203-16 (2015)
  • Masaki H, Kato-Itho M, Umino A, Sato H, Hamanaka S, Kobayashi T,Yamaguchi T, Nishimura K, Ohtaka M, Nakanishi M, Nakauchi H: Interspecific in vitro assay for chimera-forming ability of human pluripotent stem cells. Development doi:10.1242/dev.124016 (2015)
  • Takayama K, Morisaki Y, Kuno S, Nagamoto Y, Harada K, Furukawa N, Ohtaka M, Nishimura K, Imagawa K, Sakurai F, Tachibana M, Sumazaki R, Noguchi E, Nakanishi M, Hirata K, Kawabata K, Mizuguchi H: Prediction of inter-individual differences in hepatic functions and drug sensitivity by using human iPS-derived hepatocytes. Proc. Natl. Acad. Sci. USA. Vol. 111(47), 16772-16777 (2014)
  • Trokovic R, Weltner J, Nishimura K, Ohtaka M, Nakanishi M, Salomaa V, Jalanko A, Otonkoski T, A Kyttälä: Advanced Feeder-Free Generation of iPSC Directly From Blood Cells. Stem Cells Trans. Med. Vol. 3(12), 1402-1409 (2014)
  • Nishimura K, Kato T, Chen C, Oinam L, Shiomitsu E, Ayakawa D, Ohtaka M, Fukuda A, Nakanishi M, Hisatake K: Manipulation of KLF4 Expression Generates iPSCs Paused at Successive Stages of Reprogramming.Stem Cell Reports Vol. 3(5), 915-929 (2014)
  • Kogawa M, Hisatake K, Atkins GJ, Findlay DM, Enoki Y, Sato T, Gray PC, Kanesaki-Yatsuka Y, Anerson PH, Wada S, Kato N, Fukuda A, Katayama S, Tsujimoto M, Yoda T, Suda T, Okazaki Y, Matsumoto M: The Paired-box Domain Transcription Factor Pax6 Binds to the Upstream Region of the TRAP Gene Promoter and Suppresses RANKL-induced Osteoclast Differentiation. J. Biol. Chem. doi:10.1074/jbc.M113.461848 (2013)
  • Fukuda A, Shimada M, Nakadai T, Nishimura K, Hisatake K: Heterogeneous Nuclear Ribonucleoprotein R Cooperates with Mediator to Facilitate Transcription Reinitiation on the c-Fos Gene. PLoS ONE 8(8): e72496. doi:10.1371/journal.pone.0072496 (2013)
  • Wakao H, Yoshikiyo K, Koshimizu U, Furukawa T, Enomoto K, Matsunaga T, Tanaka T, Yasutomi Y, Yamada T, Minakami H, Tanaka J, Oda A, Sasaki T, Wakao R, Lantz O, Udagawa T, Sekiya Y, Higuchi K, Harada N, Nishimura K, Ohtaka M, Nakanishi M, Fujita H: Expansion of Functional Human Mucosal-Associated Invariant T Cells via Reprogramming to Pluripotency and Redifferentiation. Cell Stem Cell 12, 546-558 (2013)
  • Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Katsumata A, Nishimura K, Ohtaka M, Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T: GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis in germline stem cells. RNA 19, 803-810 (2013)
  • Tateno H, Matsushima A, Hiemori K, Onuma Y, Ito Y, Hasehira K, Nishimura K, Ohtaka M, Takayasu S, Nakanishi M, Ikehara Y, Nakanishi M, Ohnuma K, Chan T, Toyoda M, Akutsu H, Umezawa A, Asashima M, Hirabayashi J: Podocalyxin is a Glycoprotein Ligand of the Human Pluripotent Stem Cell-Specific Probe rBC2LCN. Stem Cells Trans. Med. doi:10.5966/stcm.2012-0154 (2013)
  • Nishimura T, Kaneko S, Kawana-Tachikawa A, Tajima Y, Goto H, Zhu D, Nakayama-Hosoya K, Iriguchi S, Uemura Y, Shimizu T, Takayama N, Yamada D, Nishimura K, Ohtaka M, Watanabe N, Takahashi S, Iwamoto A, Koseki H, Nakanishi M, Eto K, Nakauchi H: Generation of rejuvenated antigen-specific T cells by reprogramming to pluripotency and redifferentiation. Cell Stem Cell 12, 114-126 (2013).
  • Nishimura K, Sano M, Ohtaka M, Furuta B, Umemura Y, Nakajima Y, Ikehara Y, Kobayashi T, Segawa H, Takayasu S, Sato H, Motomura K, Uchida E, Kanayasu-Toyoda T, Asashima M, Nakauchi H, Yamaguchi T, Nakanishi M: Development of defective and persistent Sendai virus vector: a unique gene delivery/expression system ideal for cell reprogramming. J. Biol. Chem. 286, 4760-4771 (2011).
  • Takayama N, Nishimura S, Nakamura S, Shimizu T, Ohnishi R, Endo H, Yamaguchi T, Otsu M, Nishimura K, Nakanishi M, Sawaguchi A, Nagai R, Takahashi K, Yamanaka S, Nakauchi H, Eto K: Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells. J. Exp. Med. 207, 2817-2830 (2010).
  • Shimada M, Nakadai T, Fukuda A, Hisatake K: cAMP-response element-binding protein (CREB) controls MSK1-mediated phosphorylation of histone H3 at the c-fos promoter in vitro. J. Biol. Chem. 285, 9390-9401 (2010).
  • Chen Y, Yamaguchi Y, Tsugeno Y, Yamamoto J, Yamada T, Nakamura M, Hisatake K, Handa H: DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation. Genes Dev. 23, 2765-2777 (2009).
  • Fukuda A, Nakadai T, Shimada M, Hisatake K: Heterogeneous nuclear ribonucleoprotein R enhances transcription from the naturally configured c-fos promoter in vitro. J. Biol. Chem. 284, 23472-23480 (2009).
  • Yamagata K, Daitoku H, Takahashi Y, Namiki K, Hisatake K, Kako K, Mukai H, Kasuya Y, Fukamizu A: Arginine methylation of FOXO transcription factors inhibits their phosphorylation by Akt. Mol. Cell 32, 221-231 (2008).
  • Nishimura K, Segawa H, Goto T, Morishita M, Masago A, Takahashi H, Ohmiya Y, Sakaguchi T, M. Asada M, Imamura T, Shimotono K, Takayama K, Yoshida T, Nakanishi M: Persistent and stable gene expression by a cytoplasmic RNA replicon based on a noncytopathic variant Sendai virus. J. Biol. Chem. 282, 27383-27391 (2007).
  • Goto T, Morishita M, Nishimura K, Nakanishi M, Kato A, Ehara J, Takayama K: Novel mucosal insulin delivery systems based on fusogenic liposomes. Pharm. Res. 23, 384-391 (2006).
  • Zhang X, Krutchinsky A, Fukuda A, Chen W, Yamamura S, Chait BT, Roeder RG: MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. Mol. Cell 19, 89-100 (2005).
  • Dou Y, Milne TA, Tackett AJ, Smith ER, Fukuda A, Wysocka J, Allis CD, Chait BT, Hess JL, Roeder RG: Physical Association and Coordinate Function of the H3 K4 Methyltransferase MLL1 and the H4 K16 Acetyltransferase MOF. Cell 121, 873-885 (2005).
  • Imazawa Y, Hisatake K, Mitsuzawa H, Matsumoto M, Tsukui T, Nakagawa K, Nakadai T, Shimada M, Ishihama A, Nogi Y: The fission yeast protein, Ker1p, is an ortholog of RNA polymerase I subunit A14 in Saccharomyces cerevisiae, and is required for stable association of Rrn3p and RPA21 in Pol I. J. Biol. Chem. 280, 11467-11474 (2005).
  • Fukuda A, Nakadai T, Shimada M, Tsukui T, Matsumoto M, Nogi Y, Meisterernst M, Hisatake K: Transcriptional coactivator PC4 stimulates promoter escape and facilitates transcriptional synergy by GAL4-VP16. Mol. Cell. Biol. 24, 6525-6535 (2004).
  • Nishimura K, Ueda K, Guwanan E, Sakakibara S, Do E, Ohsaki E, Yada K, Okuno T, Yamanishi K: A posttranscriptional regulator of Kaposi's sarcoma-associated herpesvirus interacts with RNA-binding protein PCBP1 and controls gene expression through the IRES. Virology 325, 364-378 (2004).
  • Matsumoto M, Kogawa M, Wada S, Takayanagi H, Tsujimoto M, Katayama S, Hisatake K, Nogi Y: Essential role of p38 MAP kinase in Cathepsin K gene expression during osteoclastogenesis through association of NFATc and PU.1. J. Biol. Chem. 279, 45969-45979 (2004).
  • Sakakibara S, Ueda K, Nishimura K, Do E, Ohsaki E, Okuno T, Yamanishi K: Accumulation of heterochromatin components on the terminal repeat sequence of Kaposi's sarcoma-associated herpesvirus mediated by the latency-associated nuclear antigen. J. Virol. 78, 7299-7310 (2004).
  • Fukuda A, Tokonabe S, Hamada M, Matsumoto M, Tsukui T, Nogi Y, Hisatake K: Alleviation of PC4-mediated transcriptional repression by the ERCC3 helicase activity of general transcription factor TFIIH. J. Biol. Chem. 278,14827-14831 (2003).
  • Nishimura K, Ueda K, Sakakibara S, Do E, Ohsaki E, Okuno T, Yamanishi K: A viral transcriptional activator of Kaposi's sarcoma-associated herpesvirus (KSHV) induces apoptosis, which is blocked in KSHV-infected cells. Virology 316, 64-74 (2003).
  • Okuno T, Jiang YB, Ueda K, Nishimura K, Tamura T, Yamanishi K: Activation of human herpesvirus 8 open reading frame K5 independent of ORF50 expression. Virus Res. 90, 77-89 (2002).
  • Fukuda A, Nogi Y, Hisatake K: The regulatory role for the ERCC3 helicase of general transcription factor TFIIH during promoter escape in transcriptional activation. Proc. Natl. Acad. Sci. USA 99, 1206-1211 (2002).
  • Ueda K, Ishikawa K, Nishimura K, Sakakibara S, Do E, Yamanishi K: Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) replication and transcription factor activates the K9 (vIRF) gene through two distinct cis elements by a non-DNA-binding mechanism. J. Virol. 76, 12044-12054, (2002).
  • Matsumoto M, Hisatake K, Nogi Y, Tsujimoto M: Regulation of receptor activator of NF-kB ligand-induced tartrate-resistant acid phosphatase gene expression by PU.1-interacting protein/interferon regulatory factor-4 synergism with microophthalmia transcription factor. J. Biol. Chem. 276, 33086-33092 (2001).
  • Nishimura K, Ueda K, Sakakibara S, Ishikawa K, Chen J, Okuno T, Yamanishi K: Functional analysis of Kaposi's sarcoma-associated herpesvirus RTA in an RTA-depressed cell line. J. Hum. Virol. 4, 296-305 (2001).
  • Fukuda A, Yamauchi J, Wu SY, Chiang CM, Muramatsu M, Hisatake K: Reconstitution of recombinant TFIIH that can mediate activator-dependent transcription. Genes Cells 6, 707-719 (2001).
  • Wada T, Orphanides G, Hasegawa J, Kim D-K, Shima D, Yamaguchi Y, Fukuda A, Hisatake K, Oh S, Reinberg D, Handa H: FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between p-TEFb and TFIIH. Mol. Cell 5, 1067-1072 (2000).
  • Ishiguro A, Nogi Y, Hisatake K, Muramatsu M, Ishihama A: The Rpb6 subunit of fission yeast RNA polymerase II is a contact target of the transcription elongation factor TFIIS. Mol. Cell. Biol. 20, 1263-1270 (2000).
  • Fondell GD, Brunel F, Hisatake K, Roeder RG: Unliganded thyroid hormone receptor a can target the TATA-binding protein for transcriptional repression. Mol. Cell. Biol. 16, 281-287 (1996).
  • Hisatake K, Ohta T, Takada R, Guermah M, Horikoshi M, Nakatani Y, Roeder RG: Evolutionary conservation of human TATA-binding-polypeptide associated factors TAFII31 and TAFII80 and interactions of TAFII80 with other TAFs and with general transcription factors. Proc. Natl. Acad. Sci. USA 92, 8195-8199 (1995).
  • Nikolov DB, Chen H, Halay ED, Usheva AA, Hisatake K, Lee DK, Roeder RG, Burley SK: Crystal structure of a TFIIB-TBP-TATA-element ternary complex. Nature 377, 119-128 (1995).
  • Suzuki T, Imagawa M, Hirabayashi M, Yuki A, Hisatake K, Muramatsu M: Identification of an enhancer responsible for a tumor marker gene expression by means of transgenic rats. Cancer Res. 55, 2651-2655 (1995).
  • Yamashita S, Hisatake K, Kokubo T, Doi K, Roeder RG, Horikoshi M, Nakatani Y: Transcription factor TFIIB sites important for interaction with promoter-bound TFIID. Science 261, 463-467 (1993).
  • Hisatake K, Roeder RG, Horikoshi M: Functional dissection of TFIIB domains required for TFIIB-TFIID-promoter complex formation and basal transcription activity. Nature 363, 744-747 (1993).
  • Hisatake K, Hasegawa S, Takada R, Nakatani Y, Horikoshi M, Roeder RG: The p250 subunit of native TATA box-binding factor TFIID is the cell cycle regulatory protein CCG1. Nature 362, 179-181 (1993).
  • Maeda Y, Hisatake K, Kondo T, Hanada K, Song C, Nishimura T, Muramatsu M: Mouse rRNA transcription factor mUBF requires both HMG-box1 and an acidic tail for nucleolar accumulation: molecular analysis of the nucleolar targeting mechanism. EMBO J. 11, 3695-3704 (1992).
  • Yamashita S, Wada K, Horikoshi M, Gong D-W, Kokubo T, Hisatake K, Yokotani N, Malik S, Roeder RG, Nakatani Y: Isolation and characterization of a cDNA encoding Drosophila transcription factor TFIIB. Proc. Natl. Acad. Sci. USA 89, 2839-2843 (1992).
  • Malik S, Hisatake K, Sumimoto H, Horikoshi M, Roeder RG: Sequence of general transcription factor TFIIB and relationships to other initiation factors. Proc. Natl. Acad. Sci. USA 88, 9553-9557 (1991).
  • Hisatake K, Nishimura T, Maeda Y, Hanada K, Song C, Muramatsu M: Cloning and structural analysis of cDNA and its gene for mouse transcription factor UBF. Nucleic Acids Res. 17, 4631-4637 (1991).
  • Ishikawa Y, Safrany G, Hisatake K, Tanaka N, Maeda Y, Kato H, Kominami R, Muramatsu M: Structure of the core promoter of human and mouse ribosomal RNA gene: Asymmetry of species-specific transcription. J. Mol. Biol. 218, 55-67 (1991).
  • Tanaka N, Kato H, Ishikawa Y, Hisatake K, Tashiro K, Kominami R, Muramatsu M: Sequence-specific binding of a transcription factor TFID to the promoter region of mouse ribosomal RNA gene. J. Biol. Chem. 256, 13836-13842 (1990).