DAJIN enables multiplex genotyping to simultaneously validate intended and unintended target genome editing outcomes
Here, we developed a genotyping method with an on-target site analysis software named Determine Allele mutations and Judge Intended genotype by Nanopore sequencer (DAJIN) that can automatically identify and classify both intended and unintended diverse mutations, including point mutations, deletions, inversions, and cis double knock-in at single-nucleotide resolution. Our approach with DAJIN can handle approximately 100 samples under different editing conditions in a single run. With its high versatility, scalability, and convenience, DAJIN-assisted multiplex genotyping may become a new standard for validating genome editing outcomes.
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PLOS Biology Published: January 18, 2022
DOI 10.1371/journal.pbio.3001507
→ Research Outline (PDF In Japanese language)