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平成12年度〜平成16年度
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A03班

井原　康夫（東京大学・大学院医学系研究科） Misonou H, Morishima-Kawashima M, Ihara Y: Oxidative stress induces intracellular accumulation of amyloid β-protein (Aβ) in human neuroblastoma cells. Biochemistry 39: 6951-6959, 2000 Saito Y, Kawai M, Inoue K, Sasaki R, Arai H, Nanba E, Kuzuhara S, Ihara Y, Kanazawa I, Murayama S: Widespread expression of alpha-synuclein and tau immunoreactivity in Hallervorden-Spatz syndrome with protracted clinical course, J Neurol Sci 177: 48-59, 2000 Gu Y, Hamajima N, Ihara Y: Neurofibrillary tangle-associated collapsin response mediator protein-2 (CRMP-2) is highly phosphorylated on Thr-509, Ser-518 and Ser-522. Biochemistry 39: 4267-4275, 2000 Gu Y, Ihara Y: Evidence that collapsin response mediator protein-2 (CRMP-2) is involved in the dynamics of microtubules. J Biol Chem 275: 17917-17920, 2000 Sawamura N, Morishima-Kawashima M, Waki H, Kobayashi K, Kuramochi T, Ito M, Oyama F, Ando S, Ihara Y: Mutant-presenilin 2-transgenic mice: A large increase in the levels of Aβ42 is presumably associated with the low-density membrane domain that contains decreased levels of glycerophospholipids and sphingomyelin. J Biol Chem 275: 27901-27908, 2000 Morishima-Kawashima M, Oshima N, Ogata H, Yamaguchi H, Yoshimura M, Sugihara S, lhara Y: Effect of apolipoprotein E allele ε4 on the initial phase of amyloid β-protein accumulation in the human brain. Am J Pathol 157: 2093-2099, 2000 Miyasaka T, Morishima-Kawashima M, Ravid R, Heutink P, van Swieten JC, Nagashima K, Ihara Y: Molecular analysis of mutant and wild-type tau deposited in the brain affected by FTDP-17 R406W mutation. Am J Pathol 158: 373-379, 2001 Yamazaki T, Chang TY, Haass C, Ihara Y: Accumulation and aggregation of amyloid β-protein in late endosomes of Niemann-Pick Type C cells. J Biol Chem 276: 4454-4460, 2001 Yamaguchi H, Sugihara S, Ogawa A, Ihara Y: Alzheimer β amyloid deposition, which is hastened by ApoE ε4 gene, precedes neurofibrillary pathology in the frontal association cortex of non-demented senior subjects. J Neuropathol Exp Neurol 60: 731-739, 2001 Oshima N, Morishima-Kawashima M, Yamaguchi H, Yoshimura M, Sugihara S, Khan K, Games D, Schenk D, Ihara Y: Accumulation of amyloid beta-protein in the low-density membrane domain accurately reflects the extent of beta-amyloid deposition in the brain. Am J Pathol 158: 2209-2218, 2001 Miyasaka T, Morishima-Kawashima M, Ravid R, Kamphorst W, Nagashima K, Ihara Y: Selective deposition of mutant tau in the FTDP-17 brain affected by the P301L mutation. J Neuropathol Exp Neurol 60: 872-884, 2001 Gu Y, Misonou H, Sato T, Dohmae N, Takio K, IharaY: Distinct intramembrane cleavage of the β-amyloid precursor protein family resembling γ-secretase-like cleavage of Notch. J Biol Chem 276: 35235-35238, 2001 Utsunomiya I, Ren J, Taguchi K, Ariga T, Tai T, Ihara Y, Miyatake T: Immunohistochemical detection of verotoxin receptors in nervous system. Brain Res Protoc 8: 99-103, 2001 Lee S, Kim JH, Lee CS, Kim JH, Kim Y, Heo K, Ihara Y, Goshima Y, Suh PG, Ryu SH: Collapsin response mediator protein-2 inhibits neuronal phospholipase D2 activity by direct interaction. J Biol Chem 277: 6542-6549, 2002 Qi Y, Morishima-Kawashima M, Sato T,Mitsumori R, lhara Y: Distinct mechanisms by mutant presenilin 1 and 2 leading to increased intracellular levels of amyloid β-protein 42 in Chinese hamster ovary cells. Biochemistry 42: 1042-1052, 2003 Sato T, Dohmae N, Qi Y, Kakuda N, Misonou H, Mitsumori R, Maruyama H, Koo EH, Haass C, Takio K, Morishima-Kawashima M, Ishiura S, Ihara Y: Potential link between amyloid β-protein 42 and C-terminal fragment γ49-99 of β-amyloid precursor protein. J Biol Chem 278: 24294-24301, 2003 Kanamori T, Nishimaki K, Asoh S, Ishibashi Y, Takata I, Kuwabara T, Taira K, Yamaguchi H, Sugihara S, Yamazaki T, Ihara Y, Nakano K, Matuda S, Ohta S: Truncated product of the bifunctional DLST gene involved in biogenesis of the respiratory chain. EMBO J 22: 2913-2923, 2003 WadaS, Morishima-KawashimaM, QiY, MisonoH, ShimadaY, Ohno-IwashitaY, Ihara Y: γ-Secretase activity is present in rafts, but not cholesterol-dependent. Biochemistry 42: 13977-13986, 2003 Watanabe N, Araki W, Chui DH, Makifuchi T, Ihara Y, Tabira T: Glypican-1 as an Aβ binding HSPG in the human brain: Its localization in DIG domains and possible roles in the pathogenesis of Alzheimer's disease. FASEB J 18: 1013-1015, 2004 Oyama F, Kotliarova S, Harada A, Ito M, Miyazaki H, Ueyama Y, Hirokawa N, NukinaN, Ihara Y: Gem GTPase and tau: morphological changes induced by Gem GTPase in CHO cells are antagonized by tau. J Biol Chem 279: 27272-27277, 2004 Watanabe A, Hong W-K, Dohmae N, Takio K, Morishima-Kawashima M, Ihara Y: Molecular aging of tau: disulfide-independent aggregation and nonenzymatic degradation in vitro and in vivo. J Neurochem 90: 1302-1311, 2004 FunamotoS, Morishima-KawashimaM, TanimuraY, HirotaniN, SaidoTC, IharaY: Truncated carboxyl terminal fragments of β-amyloid precursor protein are processed to amyloid β-proteins 40 and 42. Biochemistry 43: 13532-13540, 2004 Qi-Takahara Y, Morishima-Kawashima M, Tanimura Y, Hirotani N, Horikoshi Y, Maeda M, Saido TC, Ihara Y: Longer forms of amyloid β-protein: implications for the mechanism of intramembranous cleavage by γ-secretase. J Neurosci 25: 436-445, 2005 Uchida Y, Ohshima T, Sasaki Y, Suzuki H, Yanai S, Yamashita N, Nakamura F, Takei K, Ihara Y, Mikoshiba K, Kolattukudy P, Honnorat J, Goshima Y: Semaphorin3A signalling is mediated via sequential Cdk5 and GSK3beta phosphorylation of CRMP2: implication of common phosphorylating mechanism underlying axon guidance and Alzheimer's disease. Genes Cells 10: 165-179, 2005 Katsuno T, Morishima-Kawashima M, Saito Y, Yamanouchi H, Ishiura S, Murayama S, Ihara Y: Independent accumulations of tau and amyloidâ-protein in the human entorhinal cortex. Neurology 64: 687-692, 2005 Miyasaka T, Ding Z, Gengyo-Ando K, Oue M, Yamaguchi H, Mitani S, Ihara Y: Progressive neurodegeneration in C. elegans model of tauopathy. Neurobiol Dis 20:372-383,2005 Nakaya Y, Yamane T, Shiraishi H, Wang HQ, Matsubara E, Sato T, Dolios G, Wang R, De Strooper B, Shoji M, Komano H, Yanagisawa K, Ihara Y, Fraser P, St George-Hyslop P, Nishimura M: Random mutagenesis of presenilin 1 identifies novel mutants exclusively generating long amyloid beta peptides. J Biol Chem 280: 19070-19077, 2005 Sato T, Tanimura Y, Hirotani N, Saido TC, Morishima-Kawasdhima M, Ihara Y: Blocking the cleavage at midportion between γ- and ε-sites remarkably suppresses the generation of amyloid β-protein. FEBS Lett 579: 2907-2912, 2005 Miyasaka T, Watanabe A, Saito Y, Murayama S, Mann DMA, Yamazaki M, Ravid R, Morishima-Kawashima M, Nagashima K, Ihara Y: Visualization of newly deposited tau in neurofibrillary tangles and neuropil threads. J Neuropathol Exp Neurol 64: 665-674, 2005 田平　武　（国立長寿医療研究センター・研究所） Tanahashi H, Tabira T: Two RFLPs (AciI; c954C/T and FokI c1023T/C) within the coding region of the gene for human Fe65L2 inter-acting with Alzheimer’s beta-amyloid precursor protein. Hum Mutat 15: 121, 2000 Shirotani K, Takahashi K, Araki W, Tabira T: Mutational analysis of intrinsic regions of presenilin 2 which determine its endoproteolytic cleavage and pathological function. J Biol Chem 275: 3681-3686, 2000 Mizoguchi K, Yuzurihara M, Ishige A, Sasaki H, Chui DH, Tabira T: Chronic stress induces impairment of spatial working memory because of prefrontal dopaminergic dysfunction. J Neurosci 20: 1568-1574, 2000 Tanahashi H, Tabira T: Alkaline treatment after X-gal staining reaction for Escherichia coli β-galactosidase enhances sensitivity. Anal Biochem 279: 122-123, 2000 Arima K, Kowalska A, Hasegawa M, Mukoyama M, Watanabe R, Kawai M, Takahashi K, Goedert M, Iwatsubo T, Tabira T, Sunohara N: Two brothers of frontotemporal dementia and parkinsonism with an N279K mutation of the tau gene. Neurology 54: 1787-95, 2000 Kodama K, Okada S, Iseki E, Kowalska A, Tabira T, Hosoi N, Yamanouchi N, Noda S, Komatsu N, Nakazato M, Kumakiri C, Yazaki M, Sato T: Familial frontotemporal dementia with a P301L tau mutation in Japan. J Neurol Sci 176: 57-64, 2000 Tanahashi H, Tabira T: Alzheimer’s disease-associated presenilin 2 interacts with DRAL, an LIM-domain protein. Hum Mol Genet 9: 2281-2289, 2000 Sawamura N, Morishima-Kawashima M, Waki H, Kobayashi K, Kuramochi T, Frosch MP, Ding K, Ito M, Kim TW, Tanzi RE, Oyama F, Tabira T, Ando S, Ihara Y: Mutant presenilin 2 transgenic mice. A large increase in the levels of abeta 42 is presumably associated with the low density membrane domain that contains decreased levels of glycerophospholipids and sphingomyelin. J Biol Chem 275: 27901-27908, 2000 Poorkaj P, Grossman M, Steinbart E, Payami H, Sadovnick A, Nochlin D, Tabira T, Trojanowski JQ, Borson S, Galasko D, Reich S, Quinn B, Schellenberg G, Bird TD: Frequency of tau gene mutations in familial and sporadic cases of non-Alzheimer dementia. Arch Neurol 58: 383-387, 2001 Chui DH, Dobo E, Makifuchi T, Akiyama H, Kawakatsu S, Checler F, Araki W, Takahashi K, Tabira T: Apoptotoc neurons in Alzheimer’s disease frequently show intracellular Aβ42 labeling. J Alz Dis 3: 231-239, 2001 Kowalska A, Asada T, Arima K, Kumakiri C, Kozubski W, Takahashi K, Tabira T: Genetic analysis in patients with familial and sporadic frontotemporal dementia: Two tau mutations in only familial cases and no association with apolipoprotein ε4. Dement Geriatr Cogn Disord 12: 387-392, 2001 Ohnishi T, Matsuda H, Tabira T, Kogure D, Kunihiro T, Uema T, Nishikawa M, Kitayama N, Asada T, Uno M: Changes in brain morphology in Alzheimer disease and normal aging: is Alzheimer disease an exaggerated aging process? Am J Neuroradiol 22: 1680-1685, 2001 Miyamoto K, Kowalska A, Hasegawa M, Tabira T, Takahashi K, Araki W, Akiguchi I, Ikemoto A: Familial frontotemporal dementia and parkinsonism with a novel mutation at an intron 10 +11-splice site in the tau gene. Ann Neurol 50: 117-120, 2001 Tabira T: Clioquinol’s return: cautions from Japan. (letter to the editor) Science 292: 2251, 2001 Tanahashi H, Tabira T: Three novel alternatively spliced isoforms of the human beta-site amyloid precursor protein cleaving enzyme (BACE) and their effect on amyloid beta-peptide production. Neurosci Lett 307: 9-12, 2001 Mann DMA, et al & Familial Alzheimer’s Disease Study Group (Arango J, Bird T, Van Broeckhoven C, Brooks W, Brown R, Cairns N, Cras P, Ellison D, Haltia M, Li K, Jorgensen A, Krill J, Lantos P, Lippa C, Martins R, Nochlin D, Pollen D, Rosenberg C, Rossor M, Tabira T): Amyloid angiopathy and variability in amyloid beta deposition is determined by mutation position in presenilin-1-linked Alzheimer’s disease. Am J Pathol 158: 2165-2175, 2001 Araki W, Yuasa K, Takeda S, Takeda K, Shirotani K, Takahashi K, Tabira T: Proapoptotic effect of presenilin 2 (PS2) overexpression is associated with down-regulation of Bcl-2 in cultured neurons. J Neurochem 79: 1161-1168, 2001 Tabira T, Chui DH, Kuroda S: Significance of intracellular Abeta42 accumulation in Alzheimer’s disease. Front Biosci 7: a44-9, 2002 Tanahashi H, Asada T, Tabira T: c954C>T polymorphism in the Fe65L2 gene is associated with early-onset Alzheimer’s disease. Ann Neurol 52: 691-693, 2002 Tanahashi H, Tabira T: Characterization of an amyloid precursor protein (APP)-binding protein Fe65L2 and its two novel isoforms lacking phosphotyrosine interaction domain. Biochem J 367: 687-695, 2002 Tabira T, Chui DH, Nakayama H, Kuroda S, Shibuya M: Alzheimer's disease with spastic paresis and cotton wool type plaques. J Neurosci Res 70: 367-372, 2002 Konagaya M, Kato T, Sakai M, Kuru S, Matsuoka Y, Konagaya Y, Hashizume Y, Tabira T: A clinical and pathological study of a Japanese case of amyotrophic lateral sclerosis/Parkinsonism-dementia complex with family history. J Neurol 250: 164-170, 2003 Santa Y, Uyama E, Chui DH, Arima M, Kotorii S, Takahashi K, Tabira T: Genetic, clinical and pathological studies of CADASIL in Japan: a partial contribution of Notch3 mutations and implications of smooth muscle cell degeneration for the pathogenesis. J Neurol Sci 212: 79-84, 2003 Watanabe Y, Tsujimura A, Tabira T, Hashimoto-Gotoh T: Differential expression of presenilin- α and -β (PS α and PS β) in Xenopus laevis: embryonic phosphorylation of PSa. Gene 314: 165-172, 2003 Hashimoto-Gotoh T, Tsujimura A, Watanabe Y, Iwabe N, Miyata T, Tabira T: A unifying model for functional difference and redundancy of presenilin-1 and -2 in cell apoptosis and differentiation. Gene 323: 115-123, 2003 Tanahashi H, Asada T, Tabira T: Association between tau polymorphism and male early-onset Alzheimer’s disease. NeuroReport 15: 175-180, 2004 Takeda K, Wataru A, Tabira T: Enhanced generation of intracellular Aβ42 amyloid peptide by mutation of presenilins PS1 and PS2. Eur J Neurosci 19: 258-264, 2004 Mizoguchi K, Ishige A, Takeda S, Aburada M, Tabira T: Endogenous glucocorticoids are essential for maintaining prefrontal cortical cognitive function. J Neurosci 24: 5492-5499, 2004 Watanabe N, Araki W, Chui DH, Makibuchi T, Ihara Y, Tabira T: Glypican-1 as an Aβ binding HSPG in the human brain: its localization in DIG domains and possible roles in the pathogenesis of Alzheimer’s disease. FASEB J 18: 1013-1015, 2004 Hara H, Monsonego A, Yuasa K, Adachi K, Xiao X, Takeda S, Takahashi K, Weiner HL, Tabira T: Development of a safe oral Abeta vaccine using recombinant adeno-associated virus vector for Alzheimer’s disease. J Alz Dis 6: 483-488, 2004 Takeda K, Araki W, Akiyama H, Tabira T: Amino-truncated amyloid beta-peptide (Abeta5-40/42) produced from caspase-cleaved amyloid precursor protein is deposited in Alzheimer's disease brain. FASEB J 18: 1755-1757, 2004 Ohyagi Y, Asahara H, Chui DH, Tsuruta Y, Sakae N, Miyoshi K, Yamada T, Kikuchi H, Taniwaki T, Murai H, Ikezoe K, Furuya H, Kawarabayashi T, Shoji M, Checler F, Iwaki T, Makifuchi T, Takeda K, Kira JI, Tabira T: Intracellular Abeta42 activates p53 promoter: a pathway to neurodegeneration in Alzheimer's disease. FASEB J 19: 255-257, 2004 Lakshmana MK, Araki W, Tabira T: A novel death-inducing protein, AB-DIP interacts with amyloid beta protein and mediates its neurotoxicity. FASEB J 19: 1362-1364, 2005 柳澤　勝彦（国立長寿医療研究センター・研究所） Michikawa M, Gong JS, Fan QW, Sawamura N, Yanagisawa K: A novel action of Alzheimer's amyloid β-protein (Aβ)： oligomeric Aβ promotes lipid release. J Neurosci 21: 7226-7235, 2001 Kakio A, Nishimoto S, Yanagisawa K, Kozutsumi Y, Matsuzaki K: Cholesterol-dependent formation of GM1 ganglioside-bound amyloid β-protein, an endogenous seed for Alzheimer amyloid. J Biol Chem 276: 24985-24990, 2001 KawamuraY, KikuchiA, TakadaR, TakadaS, SudohS, ShibamotoS, Yanagisawa K, KomanoH: Inhibitory effect of a presenilin 1 mutation on the Wnt signalling pathway by enhancement of β-catenin phosphorylation. Eur J Biochem 268: 3036-3041, 2001 Fan QW, Yu W, Senda T, Yanagisawa K, Michikawa M: Cholesterol-dependent microtubule stabilization by modulating tau phosphorylation. J Neurochem 76: 391-400, 2001 Sawamura N, Gong JS, Garver WS, Heidenreich RA, Ninomiya H, Ohno K, Yanagisawa K, Michikawa M: Site-specific phosphorylation of tau accompanied by activation of mitogen-activated protein kinase (MAPK) in brains of Niemann-Pick type C mice. J Biol Chem 276: 10314-10319, 2001 Isobe I, Yanagisawa K, Michikawa M: 3-(4,5-Dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide (MTT) causes Akt phosphorylation and morphological changes in intracellular organellae in cultured rat astrocytes. J Neurochem 77: 274-280, 2001 Hayashi H, Igbavboa U, Hamanaka H, Kobayashi M, Fujita SC, Wood WG, Yanagisawa K: Cholesterol is increased in the exofacial leaflet of synaptic plasma membranes of human apolipoprotein E4 knock-in mice. NeuroReport 13: 383-386, 2002 Gong JS, Kobayashi M, Hayashi H, Zou K, Sawamura N, Fujita SC, Yanagisawa K, Michikawa M: Apolipoprotein E (apoE) isoform-dependent lipid release from astrocytes prepared from human-apoE3- and apoE4-knock-in mice. J Biol Chem 277: 29919-29926, 2002 Fan QW, Yu W, Gong JS, Zou K, Sawamura N, Senda T, Yanagisawa K, Michikawa M: Cholesterol-dependent modulation of dendrite outgrowth and microtubule stability in cultured neurons. J Neurochem 80: 178-190, 2002 Zou K, Gong JS, Yanagisawa K, Michikawa M: A Novel function of monomeric amyloid β-protein serving as an antioxidant molecule against metal-induced oxidative damage. J Neurosci 22: 4833-4841, 2002 Gong JS, Sawamura N, Zou K, Sakai J, Yanagisawa K, Michikawa M: Amyloid β-protein affects cholesterol metabolism in cultured neurons: Implications for pivotal role of cholesterol in the amyloid cascade. J Neurosci Res 70: 438-446, 2002 Komano H, Shiraishi H, Kawamura Y, Sai X, Suzuki R, Serneels L, Kawaichi M, Kitamura T, Yanagisawa K: A new functional screening system for identification of regulators for the generation of amyloid β-protein. J Biol Chem 277: 39627-39633, 2002 Sai X, Kawamura Y, Kokame K, Yamaguchi H, Shiraishi H, Suzuki R, Suzuki T, Kawaichi M, Miyata T, Kitamura T, De Strooper B, Yanagisawa K, Komano H: Endoplasmic reticulum stess-inducible protein, Herp, enhances presenilin-mediated generation of amyloid β-protein. J Biol Chem 277: 12915-12920, 2002 Kakio A, Nishimoto S, Yanagisawa K, Kozutsumi Y, Matsuzaki K: Interactions of amyloid β-protein with various gangliosides in raft like membranes: Importance of GM1 ganglioside-bound form as an endogenous seed for Alzheimer amyloid. Biochemistry 23: 7385-7390, 2002 Watarai M, Makino S, Michikawa M, Yanagaisawa K, Murakami S, Shirahata T: Macrophage plasma membrane cholesterol contributes to Brucella abortus infection of mice. Infect Immun 70: 4818-4825, 2002 Zou K, Kim D, Kakio A, Byun K, Gong JS, Kim M, Sawamura N, Nishimoto S, Matsuzaki K, Lee B, Yanagisawa K, Michikawa M: Amyloid β-protein (Aβ) 1-40 protects neurons from damage induced by Aβ1-42 in culture and in rat brain. J Neurochem 87: 609-619, 2003 Sawamura N, Gong JS, Chang TY, Yanagisawa K, Michikawa M: Promotion of tau phosphorylation by MAP Kinase Erk1/2 Is accompanied by reduced cholesterol level in detergent-Insoluble membrane domains in Niemann-Pick C1-deficient cells. J Neurochem 84: 1086-1096, 2003 Sai X, Kokame K, Shiraishi H, Kawamura Y, Miyata T, Yanagisawa K, Komano H: The ubiquitin-like domain of Herp is involved in Herp degradation, but not necessary for its enhancement of amyloid β-protein generation. FEBS Lett 553: 151-156, 2003 Shiraishi H, Sai X, Wang H, Maeda Y, Kuron Y, Nishimura M, Yanagisawa K, Komano H: PEN-2 enhances γ-cleavage after presenilin heterodimer formation. J Neurochem 90: 1402-1413, 2004 Yamamoto N, Igbabvoa U, Shimada Y, Ohno-Iwashita Y, Kobayashi M, Wood WG, Fujita SC, Yanagisawa K: Accelerated Aβ aggregation in the presence of GM1-ganglioside-accumulated synaptosomes of aged apoE4-knock-in mouse brain. FEBS Lett 569: 135-139, 2004 Yamamoto N, Hasegawa K, Matsuzaki K, Naiki H, Yanagisawa K: Environment- and mutation-dependent aggregation behavior of Alzheimer amyloid β-protein. J Neurochem 90: 62-69, 2004 Hayashi H, Kimura N, Yamaguchi H, Hasegawa K, Yokoseki T, Shibata M, Yamamoto N, Michikawa M, Yoshikawa Y, Terao, K Matsuzaki K, Lemere CA, Selkoe DJ, Naiki H, Yanagisawa K: A seed for Alzheimer amyloid in the brain. J Neurosci 24: 4894- 4902, 2004 Sawamura N, Ko M, Yu W, Zou K, Hanada K, Suzuki T, Gong JS, Yanagisawa K, Michikawa M: Modulation of amyloid precursor protein cleavage by cellular sphingolipids. J Biol Chem 279: 11984-11991, 2004 Yu W, Gong JS, Ko M, Garver WS, Yanagisawa K, Michikawa M: Altered cholesterol metabolism in Niemann-Pick Type C1 mouse brain affects mitochondria function. J Biol Chem 280: 11731-11739, 2005 Yu W, Zou K, Gong JS, Ko M, Yanagisawa K, Michikawa M: Oligomerization of amyloid β-protein occurs during the isolation of lipid rafts, J Neurosci Res 80: 114-119, 2005 Kimura N, Yanagisawa K, Terao K, Ono F, Sakakibara I, Ishii Y, Kyuwa S, Yoshikawa Y: Age-related changes of intracellular Aβ in cynomolgus monkey brains, Neuropathol Applied Neurobiol 31: 170-180, 2005 Yamamoto N, Hirabayashi Y, Amari M, Yamaguchi H, Romanov G, Van Nostrand WE, Yanagisawa K: Assembly of hereditary amyloid β-protein variants in the presence of favorable gangliosides. FEBS Lett 579: 2185-2190, 2005 Igbavboa U, Eckert GP, Malo TM, Studniski AE, Johnson LN, Yamamoto N, Kobayashi M, Fujita SC, Appel TR, Muller WE, Wood WG, Yanagisawa K: Murine synaptosomal lipid raft protein and lipid composition are altered by expression of human apoE 3 and 4 and by increasing age. J Neurol Sci 229-230: 225-232, 2005 石浦　章一　（東京大学・大学院総合文化研究科） Maruyama K, Usami M, Kametani F, Tomita T, Iwatsubo T, Saido TC, Mori H, Ishiura S: Molecular interactions between presenilin and calpain: inhibition of m-calpain protease activity by presenilin-1, 2 and cleavage of presenilin-1 by m-, mu-calpain. Int J Mol Med 5: 269-735, 2000 Koike H, Kouchi Z, Kinouchi T, Maeda T, Sorimachi H, Saido TC, Maruyama K, Okuyama A, Suzuki K, Ishiura S: Metabolism of amyloid precursor protein in COS cells transfected with a β-secretase candidate. Cytotechnology 33: 213-219, 2000 Tagawa K, Taya C, Hayashi Y, Nakagawa M, Ono Y, Fukuda R, Karasuyama H, Toyama-Sorimachi N, Katsui Y, Hata S, Ishiura S, Nonaka I, Seyama Y, Arahata K, Yonekawa H, Sorimachi H, Suzuki K: Myopathy phenotype of transgenic mice expressing active site-mutated inactive p94 skeletal muscle-specific calpain, the gene product responsible for limb girdle muscular dystrophy type 2A. Hum Mol Genet 9: 1393-1402, 2000 Fuke S, Suo S, Takahashi N, Koike H, Sasagawa N, Ishiura S: The VNTR polymorphism of the human dopamine transporter (DAT1) gene affects gene expression. Pharmacogenomics J 1: 152-156, 2001 Umeda T, Kouchi Z, Kawahara H, Tomioka S, Sasagawa N, Maeda T, Sorimachi H, Ishiura S, Suzuki K: Limited proteolysis of filamin is catalyzed by caspase-3 in U937 and Jurkat cells. J Biochem 130: 535-542, 2001 Ishiura S, Hotoda N, Szabo B, Asai M, Hattori C, Tanaka E, Koike H, Sasagawa N: Alzheimer amyloid α-secretase: a special target for drug development. Psychogeriatrics 1: 273-276, 2001 Suo S, Sasagawa N, Ishiura S: Identification of a dopamine receptor from Caenohabditis elegans. Neurosci Lett 319: 13-16, 2002 Suzuki T, Nakagawa M, Yoshikawa A, Sasagawa N, Yoshimori T, Ohsumi Y, Nishino I, Ishiura S, Nonaka I: The first molecular evidence that autophagy relates rimmed vacuole formation in chloroquine myopathy. J Biochem 131: 647-651, 2002 Hotoda N, Koike H, Sasagawa N, Ishiura S: A secreted form of ADAM9 has an α-secretase activity for APP. Biochem Biophys Res Commun 293: 800-805, 2002 Hattori C, Asai M, Oma Y, Kino Y, Sasagawa N, Saido TC, Maruyama K, Ishiura S: BACE1 interacts with nicastrin. Biochem Biophys Res Commun 293: 1228-1232, 2002 Asai M, Hattori C, Szabo B, Sasagawa N, Maruyama K, Tanuma S, Ishiura S: Putative function of ADAM9, ADAM10, and ADAM17 as APP α-secretase. Biochem Biophys Res Commun 301: 231-235, 2003 Yoshikawa A, Mitsuhashi H, Sasagawa N, Tsukahara T, Hayashi Y, Nishino I, Goto Y, Ishiura S: Expression of ARPP-16/19 in rat denervated skeletal muscle. J Biochem 134: 57-61, 2003 Sato T, Dohmae N, Qi Y, Kakuda N, Misonou H, Mitsumori R, Maruyama H, Koo EH, Haass C, Takio K, Morishima-Kawashima M, Ishiura S, Ihara Y: Potential link between amyloid beta protein 42 and C-terminal fragment gamma49-99 of beta amyloid precursor protein. J Biol Chem 278: 24294-24301, 2003 Suo S, Sasagawa N, Ishiura S: Cloning and characterization of a C. elegans D2-like dopamine receptor. J Neurochem 86: 869-878, 2003 Shuto D, Kasai S, Kimura T, Liu P, Hidaka K, Hamada T, Shibakawa S, Hayashi Y, Hattori C, Szabo B, Ishiura S, Kiso Y: KMI-008, a novel beta-secretase inhibitor containing a hydroxymethylcarbonyl isostere as a transition-state mimic: Design and synthesis of substrate-based octapeptides. Bioorg Med Chem Lett 13: 4273-4276, 2003 Kinouchi T, Ishiura S, Mabuchi Y, Urakami-Manaka Y, Nishio H, Nishiuchi Y, Tsunemi M, Takada K, Watanabe M, Ikeda M, Matsui H, Tomioka S, Kawahara H, Hamamoto T, Suzuki K, Kagawa Y: Mammalian D-aspartyl endopeptidase: a scavenger for noxious racemized proteins in aging. Biochem Biophys Res Commun 314: 730-736, 2004 Ito N, Nomura S, Iwase A, Ito T, Kikkawa F, Tsujimoto M, Ishiura S, Mizutani S: ADAMs, a disintegrin metalloproteinases, mediate shedding of oxytocinase. 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Nat Med 6: 143-151. 2000 Russo C, Schettini G, Saido TC, Hulette C, Lippa C, Lannfelt L, Ghetti B, Gambetti P, Tabaton M, Teller JK:  Preferential deposition of truncated amyloid-β peptides in brain of presenilin 1 gene mutation carriers. Nature 405:  531-532, 2000 Nakagawa Y, Reed L, Nakamura M, McIntosh TK, Smith DH, Saatman KE, Raghupathi R, Clemens J, Saido TC, Lee VM, Trojanowski JQ: Brain trauma in aged transgenic mice induces regression of established abeta deposits. Exp Neurol 163: 244-252, 2000 Lankiewicz S, Marc Luetjens C, Truc Bui N, Krohn AJ, Poppe M, Cole GM, Saido TC, Prehn JH: Activation of calpain I converts excitotoxic neuron death into a caspase-independent cell death. J Biol Chem 275: 17064-17071, 2000 Takeuchi A, Irizarry MC, Duff K, Saido TC, Hsiao Ashe K, Hasegawa M, Mann DM, Hyman BT, Iwatsubo T: Age-related Aβ deposition in transgenic mice overexpressing both Alzheimer mutant presenilin 1 and β amyloid precursor protein is not associated with global neuronal loss. Am J Pathol157: 331-339, 2000 Hiwasa T, Arase Y, Kikuno K, Hasegawa R, Sugaya S, Kita K, Saido T, Yamamori H, Maki M, Suzuki N: Increase in ultraviolet sensitivity by overexpression of calpastatin in ultraviolet-resistant UVr-1 cells derived from ultraviolet-sensitive human RSa cells. Cell Death Differ 7: 531-537, 2000 Iwata N, Tsubuki S, Hama E, Takaki Y, Shirotani K, Saido TC: Clearance of amyloid β-peptide from brain: transport or metabolism? Nat Med 6: 718-719, 2000 Harigaya Y, Saido TC, Eckman CB, Prada C-M, Shoji M, Younkin S: Amyloid beta protein starting pyroglutamate at position 3 is a major component of the amyloid deposits in the Alzheimer's disease brain. 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Cytotechnology 33: 213-219, 2000 Kitagaki H, Tomioka S, Yoshizawa T, Sorimachi H, Saido TC, Ishiura S, Suzuki K: Autolysis of calpain large subunit inducing irreversible dissociation of stoichiometric heterodimer of calpain. Biosci Biotechnol Biochem 64: 689-695, 2000 Shoji M, Kawarabayashi T, Sato M, Sasaki A, Saido TC, Matsubara E, Tomidokoro Y, Kanai M, Shizuka M, Ishiguro K, Ikeda M, Harigaya Y, Okamoto K, Hirai S: Age-related amyloid beta protein accumulation induces cellular death and macrophage activation in transgenic mice. J Pathol 191: 93-101, 2000 Yamaguchi A, Yamamoto N, Akamatsu N, Saido TC, Kaneda M, Umeda M, Tanoue K: PS-liposome and ox-LDL bind to different sites of the immunodominant domain of CD36: a study with GS95, a new anti-CD36 monoclonal antibody. Thromb Res 97: 317-326, 2000 Maruyama K, Usami M, Kametani F, Tomita T, Iwatsubo T, Saido TC, Mori H, Ishiura S: Molecular interactions between presenilin and calpain: inhibition of m-calpain protease activity by presenilin-1, 2 and cleavage of presenilin-1 by m-, mu-calpain. Int J Mol Med 5: 269-273, 2000 Taniguchi S, Fujita Y, Takahashi H, Hayashi S, Kakita A, Murayama S, Saido TC, Hisanaga S, Iwatsubo T, Hasegawa M: Degradation of p35 to p25 in postmortem human and rat brains. FEBS Lett 46-50, 2001 Sekine-Aizawa Y, Hama E, Watanabe K, Tsubuki S, Kanai-Azuma M, Kanai Y, Arai H, Aizawa H, Iwata N, Saido TC: Matrix metalloproteinase (MMP) system in brain: Identification and characterization of brain-specific MMP highly expressed in cerebellum. Eur J Neurosci 13: 935-948, 2001 Kiryu-Seo S, Kiyama H, Iwata H, Tomita T, Iwatsubo T, Saido TC: Neprilysin degrades both amyloid β peptides 1-40 and 1-42 most rapidly and efficiently among thiorphan- and phosphoramidon-sensitive endopeptidases. J Biol Chem 276: 21895-21901, 2001 Choi W-S, Lee E-H, Chung C-W, Jung Y-K, Jin BK, Kim SU, Oh TH, Saido TC, Oh YJ: Cleavage of Bax is mediated by caspase-dependent or -independent calpain activation in dopaminergic neuronal cells: Protective role of Bcl-2. J Neurochem 77: 1531-1541, 2001 Iwata N, Tsubuki S, Takaki Y, Shirotani K, Lu B, Gerard NP, Gerard C, Hama E, Lee H-J, Saido TC: Metabolic regulation of brain Aβ by neprilysin. Science 292: 1550-1552, 2001 Kaneko T, Yamashima T, Tohma Y, Nomura M, Imajoh-Ohmi S, Saido TC, Nakao M, Saya H, Yamamoto H, Yamashita J: Calpain-dependent proteolysis of merlin occurs by oxidative stress in meningiomas: a novel hypothesis for tumorigenesis. Cancer 92: 2662-2672, 2001 Hama E, Shirotani K, Masumoto H, Sekine-Aizawa Y, Aizawa H, Saido TC: Clearance of extracellular and cell-associated amyloid β peptide by viral expression of neprilysin in primary culture. 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Neurosci Res 43: 39-56, 2001 Shirotani K, Tsubuki S, Lee H-J, Maruyama K, Saido TC: Generation of amyloid β peptide with pyroglutamate at position 3 in primary cortical neurons. Neurosci Lett 327: 25-28, 2002 Fukami S, Iwata N, Saido TC: Therapeutic strategies of Alzheimer’s disease through manipulation of Aβ metabolism: a focus on Aβ-degrading peptidase, neprilysin. Drug Develop Res 56: 171-183, 2002 Takahashi M, Fukami S, Iwata N, Inoue K, Itohara S, Itoh H, Haraoka J, Saido TC: In vivo glioma growth requires host-derived matrix metalloproteinase 2 for maintenance of angioarchitecture. Pharmacol Res 46: 155-163, 2002 Iwata N, Takaki Y, Fukami S, Tsubuki S, Saido TC: Region-specific reduction of Aβ-degrading endopeptidase, neprilysin, in mouse hippocampus upon aging. J Neurosci Res 70: 493-500, 2002 Hattori C, Asai M, Oma Y, Kino Y, Sasagawa N, Saido TC, Maruyama K, Ishiura S: BACE1 interacts with nicastrin. Biochem Biophys Res Commun 293: 228-1232, 2002 Tomioka M, Shirotani K, Iwata N, Lee HJ, Yang F, Cole GM, Seyama Y, Saido TC: In vivo role of caspases in excitotoxic neuronal death: generation and analysis of transgenic mice expressing baculoviral caspase inhibitor, p35, in postnatal brain. Mol Brain Res 108: 18-32, 2002 Mori C, Spooner ET, Wisniewski KE, Wisniewski TM, Yamaguchi H, Saido TC, Tolan DR, Selkoe DJ, Lemere CA: Intraneuronal Aβ42 accumulation in Down syndrome brain. Amyloid 9: 88-102, 2002 Kuwano K, Nisimura I, Uetsuki T, Saido TC, Yoshikawa K: Activation of calpain in cultured neurons overexpressing Alzheimer amyloid precursor protein. Mol Brain Res 107: 166-175, 2002 Dare E, Tofighi R, Vettori MV, Momoi T, Poli D, Saido TC, Mutti A, Ceccatelli S: Styrene 7,8-oxide induces caspase activation and regular DNA fragmentation in neuronal cells. 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J Biol Chem 275: 36794-36802, 2000 Fraser PE, Yang D, Yu G, Levesque L, Nishimura M, Arawaka S, Serpell LC, Rogaeva E, St George-Hyslop P: Presenilin structure, function and role in Alzheimer disease. Biochim Biophys Acta 1502: 1-15, 2000 Chen F, Yu G, Arawaka S, Nishimura M, Kawarai T, Yu H, Tandon A, Supala A, Song YQ, Rogaeva E, Milman P, Sato C, Yu C, Janus C, Lee J, Song L, Zhang L, Fraser PE, St George-Hyslop PH: Nicastrin binds to membrane-tethered Notch. Nat Cell Biol 3: 751-754, 2001 Rozmahel R, Huang J, Chen F, Liang Y, Nguyen V, Ikeda M, Levesque G, Yu G, Nishimura M, Mathews P, Schmidt SD, Mercken M, Bergeron C, Westaway D, St George-Hyslop P: Normal brain development in PS1 hypomorphic mice with markedly reduced γ-secretase cleavage of βAPP. Neurobiol Aging 23: 187-194, 2002 Wang HQ, Takebayashi K, Tsuchida K, Nishimura M, Noda Y: Follistatin-related gene (FLRG) expression in human endometrium: sex steroid hormones regulate the expression of FLRG in cultured human endometrial stromal cells. J Clin Endocrinol Metab 88: 4432-4439, 2003 Araki Y, Miyagi N, Kato N, Yoshida T, Wada S, Nishimura M, Komano H, Yamamoto T, De Strooper B, Yamamoto K, Suzuki T: Coordinated metabolism of Alcadein and amyloid β-protein precursor regulates FE65-dependent gene transactivation. J Biol Chem 279: 24343-24354, 2004 Shiraishi H, Sai X, Wang HQ, Maeda Y, Kurono Y, Nishimura M, Yanagisawa K, Komano H: PEN-2 enhances γ-cleavage after presenilin heterodimer formation. 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Am J Pathol 166: 521-531, 2005 Matsubara E, Sekijima Y, Tokuda T, Urakami K, Amari M, Shizuka-Ikeda M, Tomidokoro Y, Ikeda M, Kawarabayashi T, Harigaya Y, Ikeda S, Murakami T, Abe K, Otomo E, Hirai S, Frangione B, Ghiso J, Shoji M: Soluble Abeta homeostasis in AD and DS: impairment of anti-amyloidogenic protection by lipoproteins. Neurobiol Aging 25: 833-841, 2004 Ikarashi Y, Harigaya Y, Tomidokoro Y, Kanai M, Ikeda M, Matsubara E, Kawarabayashi T, Kuribara H, Younkin SG, Maruyama Y, Shoji M: Decreased level of brain acetylcholine and memory disturbance in APPsw mice. Neurobiol Aging 25: 483-490, 2004 石井　一弘　（筑波大学・臨床医学系） Ishii K, Lippa C, Tomiyama T, Miyatake F, Ozawa K, Tamaoka A, Hasegawa T, Fraser PE, Shoji S, Nee LE, Pollen DA, St George-Hyslop PH, Ii K, Ohtake T, Kalaria RN, Rossor MN, Lantos PL, Cairns NJ, Farrer LA, Mori H: Distinguishable effects of presenilin-1 and APP717 mutations on amyloid plaque deposition. Neurobiol Aging 22:  367-376, 2001 Ishii K, Tamaoka A, Shoji S: A case of primary focal lingual dystonia induced by speaking. Eur J Neurol 8: 507, 2001 Ishii K, Klunk WE, Arawaka S, Debnath ML, Furiya Y, Sahara N, Shoji S, Tamaoka A, Pettegrew JW, Mori H: Chrysamine G and its derivative reduce amyloid beta-induced neurotoxicity in mice. Neurosci Lett 333: 5-8, 2002 Tokuda T, Oide T, Tamaoka A, Ishii K, Matsuno S, Ikeda S: Prednisolone (30-60 mg/day) for diseases other than AD decreases amyloid beta-peptides in CSF. Neurology 58: 1415-1418, 2002 井出　千束　（京都大学・大学院医学研究科） Asai K, Fujimoto K, Harazaki M, Kusunoki T, Korematsu S, Ide C, Ra C, Hosoi S: Distinct aggregation of beta- and gamma-chains of the high-affinity IgE receptor on cross-linking. J Histochem Cytochem 48: 1705-1716, 2000 Chakrabortty S, Kitada M, Matsumoto N, Taketomi M, Kimura K, Ide C: Choroid plexus ependymal cells enhance the neurite outgrowth from dorsal root ganglion neurons in vitro. 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Mol Cell Biol Res Commun 4: 43-49, 2000 Nakaya T, Kawahara S, Watanabe S, Lee D-S, Suzuki T, Kirino Y: Expression and function of a novel gene in odor-taste associative learning in the terrestrial slug. Genes Cells 6: 43-56, 2001 Satoh Y, Hirakura Y, Shibayama S, Hirashima N, Suzuki T, Kirino Y: β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from an electric ray. Neuosci Lett 302: 97-100, 2001 Ando K, Iijima K, Elliott JI, Kirino Y, Suzuki T: Phosphorylation-dependent regulation of the interaction of amyloid precursor protein with Fe65 affects the production of β-amyloid. J Biol Chem 276: 40353-40361, 2001 Sai X, Kawamura Y, Kokame K, Yamaguchi H, Shiraishi H, Suzuki R, Suzuki T, Kawaichi M, Miyata T, Kitamura T, De Strooper B, Yanagisawa K, Komano H: Endoplasmic reticulum stress-inducible protein, Herp, enhances presenilin-mediated generation of amyloid β-protein. 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J Biol Chem277: 39696-39702, 2000 Inomata H, Nakamura Y, Hayakawa A, Takata H, Suzuki T, Miyazawa K, Kitamura N: A scaffold protein JIP-1b enhances amyloid precursor protein phosphorylation by JNK and its association with kinesin light chain 1. J Biol Chem 278: 22946-22955, 2003 Sumioka A, Imoto S, Martins RN, Kirino Y, Suzuki T: XB51 isoforms mediate Alzheimer’s β-amyloid peptide production by X11L-dependent and -independent mechanisms. Biochem J 374: 261-268, 2003 Araki Y, Tomita S, Yamaguchi H, Miyagi N, Sumioka A, Kirino Y, Suzuki T: Novel cadherin-related membrane proteins, Alcadeins, enhance the X11-like protein mediated stabilization of amyloid β-protein precursor metabolism.J Biol Chem278: 49448-49458, 2003 Sawamura N, Mihee K, Wenxin Y, Zou K, Handa K, Suzuki T, Gong J-S, Yanagisawa K, Michikawa M: Modulation of amyloid precursor protein cleavage by cellular sphingolipids. J Biol Chem 279: 11984-11991, 2004 Taru H, Suzuki T: Facilitation of stress-induced phosphorylation of β-amyloid precursor protein family members by X11-like/Mint2 protein. J Biol Chem 279: 21628-21636, 2004 Tabuchi A, Ishii A, Fukuchi M, Kobayashi S, Suzuki T, Tsuda M:Activity-dependent increase in β-amyloid precursor protein mRNA expression in neurons. NeuroReport15: 1329-1333, 2004 Araki Y, Miyagi N, Kato N, Yoshida T, Wada S, Nishimura M, Komano H, Yamamoto T, De Strooper B, Yamamoto K, Suzuki T: Coordinated metabolism of Alcadein and amyloid β-protein precursor regulates FE65-dependent gene transactivation. J Biol Chem 279: 24343-24354, 2004 Taru H, Yoshikawa K, Suzuki T: Caspase cleavage of β-amyloid precursor protein is regulated by cytoplasmic phosphorylation. FEBS Lett567: 248-252, 2004 Von Rotz RC, Kohli BM, Bosset J, Meier M, Suzuki T, Nitsch RM, Konietzko U: The APP intracellular domain forms nuclear multiprotein complexes and regulates the transcription of its own precursor. J Cell Sci117: 4435-4448, 2004 Asaumi M, Iijima K, Sumioka A, Iijima-Ando K, Kirino Y, Nakaya T, Suzuki T: Interaction of N-terminal acetyltransferase with the cytoplasmic domain of β-amyloid precursor protein and its effect on Aβ secretion. J Biochem 137: 147-155, 2005 住本　英樹　（九州大学・生体防御医学研究所） Shiose A, Sumimoto H: Arachidonic acid and phosphorylation synergistically induce a conformational change of p47phox to activate the phagocyte NADPH oxidase. J Biol Chem 275: 13793-13801, 2000 Takeya R, Takeshige K, Sumimoto H: Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors. Biochem Biophys Res Commun 267: 149-155, 2000 Tanaka H, Takeya R, Sumimoto H: A novel intracellular membrane-bound calcium-independent phospholipase A2. Biochem Biophys Res Commun 272: 320-326, 2000 Shiose A, Kuroda J, Tsuruya K, Hirai M, Hirakata H, Naito S, Hattori M, Sakaki Y, Sumimoto H: A novel superoxide-producing NAD(P)H oxidase in kidney. J Biol Chem 276: 1417-1423, 2001 Noda Y, Takeya R, Ohno S, Naito S, Ito T, Sumimoto H: Human homologues of the Caenorhabditis eleganscell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C. Genes Cells 6: 107-119, 2001 Hiroaki H, Ago T, Ito T, Sumimoto H, Kohda D: Solution structure of the PX domain, a target of the SH3 domain. Nat Struct Biol 8: 526-530, 2001 Nosaka Y, Arai A, Kanda E, Akasaki T, Sumimoto H, Miyasaka N, Miura O: Rac is activated by tumor necrosis factor α and is involved in activation of Erk. Biochem Biophys Res Commun 285: 675-679, 2001 Ito T, Matsui Y, Ago T, Ota K, Sumimoto H: Novel modular domain PB1 recognizes PC motif to mediate functional protein-protein interactions. EMBO J 20: 3938-3946, 2001 Terasawa H, Noda Y, Ito T, Hatanaka H, Ichikawa S, Ogura K, Sumimoto H, Inagaki F: Structure and ligand recognition of the PB1 domain: a novel protein module capable of binding PC motif-containing protein. EMBO J 20: 3947-3956, 2001 Ago T, Takeya R, Hiroaki H, Kuribayashi F, Ito T, Kohda D, Sumimoto H: The PX domain as a novel phosphoinositide-binding module. Biochem Biophys Res Commun 287: 733-738, 2001 Yamamori T, Inanami O, Sumimoto H, Akasaki T, Nagahata H, Kuwabara M: Relationship between p38 mitogen-activated protein kinase and small GTPase Rac for the activation of NADPH oxidase in bovine neutrophils. Biochem Biophys Res Commun 293: 1571-1578, 2000 Kami K, Takeya R, Sumimoto H, Kohda D: Diverse recognition of non-PxxP peptide ligands by the SH3 domains from p67phox, Grb2 and Pex13p. EMBO J 21: 4268-4276, 2000 Kubo T, Yamashita T, Yamaguchi A, Sumimoto H, Hosokawa K, Tohyama M: A novel FERM domain including guanine nucleotide exchange factor is involved in Rac signaling and regulates neurite remodeling. J Neurosci 22: 8504-8513, 2000 Kuribayashi F, Nunoi H, Wakamatsu K, Tsunawaki S, Sato K, Ito T, Sumimoto H: The adaptor protein p40phox as a positive regulator of the superoxide-producing phagocyte oxidase. EMBO J 21: 6312-6320, 2000 Kohjima M, Noda Y, Takeya R, Saito N, Takeuchi K, Sumimoto H: PAR3β, a novel homologue of the cell polarity protein PAR3, localizes to tight junctions. Biochem Biophys Res Commun 299: 641-646, 2000 Ago T, Kuribayashi F, Hiroaki H, Takeya R, Ito T, Kohda D, Sumimoto H: Phosphorylation of p47phox directs phox homology domain from SH3 domain toward phosphoinositides, leading to phagocyte NADPH oxidase activation. Proc Natl Acad Sci USA 100: 4474-4479, 2003 Takeya R, Ueno N, Kami K, Taura M, Kohjima M, Izaki T, Nunoi H, Sumimoto H: Novel human homologues of p47phox and p67phox participate in activation of superoxide-producing NADPH oxidases. J Biol Chem 278: 25234-25246, 2003 Inoguchi T, Tsubouchi H, Etoh T, Kakimoto M, Sonta T, Utsumi H, Sumimoto H, Yu HY, Sonoda N, Inuo M, Sato N, Sekiguchi N, Kobayashi K, Nawata H: A possible target of antioxidative therapy for diabetic vascular complications-vascular NAD(P)H oxidase. Curr Med Chem10: 1759-1764, 2003 Yuzawa S, Suzuki NN, Fujioka Y, Ogura K, Sumimoto H, Inagaki F: Crystallization and preliminary crystallographic analysis of autoinhibited form of tandem SH3 domain of p47phox. Acta Crystallogr D Biol Crystallogr 59: 1479-1480, 2003 Etoh T, Inoguchi T, Kakimoto M, Sonoda N, Kobayashi K, Kuroda J, Sumimoto H, Nawata H: Increased expression of NAD(P)H oxidase subunits, NOX4 and p22phox, in kidney of streptozotocin-induced diabetic rats and its reversibility in interventive insulin treatment. Diabetologia 46: 1428-1437, 2003 Takeya R, Sumimoto H: Fhos, a mammalian formin, directly binds to F-actin via the N-terminal region and forms a homotypic complex via the FH2 domain to promote actin fiber formation. J Cell Sci 116: 4567-4575, 2003 Yoshinaga S, Kohjima M, Ogura K, Yokochi M, Takeya R, Ito T, Sumimoto H, Inagaki F: The PB1 domain and the PC motif-containing region are structurally similar protein binding modules. EMBO J 22: 4888-4897, 2003 Noda Y, Kohjima M, Izaki T, Ota K, Yoshinaga S, Inagaki F, Ito T, Sumimoto H: Molecular recognition in dimerization between PB1 domains. J Biol Chem 278: 43516-43524, 2003 Mizukami Y, Sumimoto H, Takeshige K: Induction of cytochrome CYP4F3A in all-trans-retinoic acid-treated HL60 cells. Biochem Biophys Res Commun 314: 104-109, 2004 Kawahara T, Kuwano Y, Teshima-Kondo S, Takeya R, Sumimoto H, Kishi K, Tsunawaki S, Hirayama T, Rokutan K: Role of NADPH oxidase 1 in oxidative burst response to Toll-like receptor 5 signaling in large intestinal epithelial cells. J Immunol 172: 3051-3058, 2004 Yuzawa S, Suzuki NN, Fujioka Y, Ogura K, Sumimoto H, Inagaki F: A molecular mechanism for autoinhibition of the tandem SH3 domains of p47phox, the regulatory subunit of the phagocyte NADPH oxidase. Genes Cells 9: 443-456, 2004 Hashida S, Yuzawa S, Suzuki NN, Fujioka Y, Takikawa T, Sumimoto H, Inagaki F, Fujii H: Binding of FAD to cytochrome b558 is facilitated during activation of the phagocyte NADPH oxidase, leading to superoxide production. J Biol Chem 279: 26378-26386, 2004 武田　篤　（東北大学・大学院医学系研究科） Takeda A, Perry G, Abraham NG, Dwyer BE, Kutty RK, Laitinen JT, Petersen RB, Smith MA: Overexpression of heme oxygenase in neuronal cells, the possible interaction with tau. J Biol Chem 275: 5395-5399, 2000 Gerst JL, Raina AK, Pirim I, Mcshea A, Harris PL, Siedlak SL, Takeda A, Petersen RB, Smith MA: Altered cell-matrix associated ADAM proteins in Alzheimer disease. J Neurosci Res 59: 680-684, 2000 Tateyama M, Tobita M, Takeda A, Chida K, Onodera Y, Kikuchi A, Aoyagi N, Itoyama Y: DNA single-strand breaks are increased in muscle diseases with rimmed vacuoles. Acta Neuropathol 100: 390-394, 2000 Kimpara T, Takeda A, Yamaguchi T, Arai H, Okita N, Takase S, Sasaki H, Itoyama Y: Increased bilirubin and its derivatives in cerebrospinal fluid in Alzheimer’s disease. 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J Neurochem 79: 311-318, 2001 Zhu X, Castellani RJ, Takeda A, Nunomura A, Atwood CS, Perry G, Smith MA: Differential activation of neuronal ERK, JNK/SAPK and p38 in Alzheimer’s disease: “two hit” hypothesis. Mech Ageing Dev 123: 39-46, 2001 武田　雅俊　（大阪大学・大学院医学系研究科） Sato N, Imaizumi K, Manabe T, Taniguchi M, Hitomi J, Katayama T, Yoneda T, Morihara T, Yasuda Y, Takagi T, Kudo T, Tsuda T, Itoyama Y, Makifuchi T, Fraser EP, St. George-Hyslop P, Tohyama M: Increased production of β-amyloid and vulnerability to endoplasmic reticulum stress by an aberrant spliced form of presenilin 2. 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J Health Sci 51: 130-137. 2005 玉村　啓和　（京都大学・薬学研究科） Otaka A, Watanabe H, Mitsuyama E, Yukimasa A, Tamamura H, Fujii N: Synthesis of (Z)-fluoroalkene dipeptide isosteres utilizing organocopper-mediated reduction of γ, γ -difluoro-α,β-enoates. Tetrahedron Lett 42: 285-287, 2001 Tamamura H, Sugioka M, Odagaki Y, Omagari A, Kan Y, Oishi S, Nakashima H, Yamamoto N, Stephen CP, Hamanaka N, Otaka A, Nobutaka F: Conformational study of a highly specific CXCR4 inhibitor, T140, disclosing the close proximity of its intrinsic pharmacophores associated with strong anti-HIV activity. Bioorg Med Chem Lett 11: 359-362, 2001 Gotoh K, Yoshimori M, Kanbara K, Tamamura H, Kanamoto T, Mochizuki K, Fujii N, Nakashima H: Increase of R5 HIV-1 infection and CCR5 expression in T cells treated with high concentrations of CXCR4 antagonists and SDF-1. J Infect Chemother 7: 28-36, 2001 Kanbara K, Sato S, Tanuma J, Tamamura H, Gotoh K, Yoshimori M, Kanamoto T, Kitano M, Fujii N, Nakashima H: Biological and genetic characterization of a human immunodeficiency virus strain resistant to CXCR4 antagonist T134. AIDS Res Hum Retroviruses 17: 615-622, 2001 Tamamura H, Omagari A, Hiramatsu K, Gotoh K, Kanamoto T, Xu Y, Kodama E, Matsuoka M, Hattori T, Yamamoto N, Nakashima H, Otaka A, Fujii N: Development of specific CXCR4 inhibitors possessing high selectivity indexes as well as complete stability in serum based on an anti-HIV peptide T140. Bioorg Med Chem Lett 11: 1897-1902, 2001 Tamamura H, Omagari A, Hiramatsu K, Kanamoto T, Gotoh K, Kanbara K, Yamamoto N, Nakashima H, Otaka A, Fujii N: Synthesis and evaluation of bifunctional anti-HIV agents based on specific CXCR4 antagonists-AZT conjugation. 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J Med Chem 46: 1764-1768, 2003 Yasuda T, Poole AR, Shimizu M, Nakagawa T, Julovi MS, Tamamura H, Fujii N, Nakamura T: Involvement of CD44 in induction of matrix metalloproteinases by a COOH-terminal heparin-binding fragment of fibronectin in human articular cartilage in culture. Arthritis Rheum 48: 1271-1280, 2003 Otaka A, Yukimasa A, Watanabe J, Sasaki Y, Oishi S, Tamamura H, Fujii N: Application of samarium diiodide (SmI2)-induced reduction of γ-acetoxy-α,β-enoates with α-specific kinetic electrophilic trapping for the synthesis of amino acid derivatives. Chem Commun 2003: 1834-1835, 2003 Tamamura H, Kato T, Otaka A, Fujii N: Synthesis of potent beta-secretase inhibitors containing a hydroxyethylamine dipeptide isostere and their structure-activity relationship studies. 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Neurosci Lett 326: 9-12, 2002 Sasaki A, Shoji M, Harigaya Y, Kawarabayashi T, Ikeda M, Naito M, Matsubara E, Abe K, Nakazato Y: Amyloid cored plaques in Tg2576 transgenic mice are characterized by giant plaques, slightly activated microglia, and the lack of paired helical filament-typed, dystrophic neurites. Virchows Arch 441: 58-67, 2002 Matsubara E, Bryant-Thomas T, Pacheco J, enry TL, Poeggeler B, Manjon M, Herbert D, Cruz-Sanchez F, Chyan Y-J, Shoji M, Abe K, Leone A, Grundke-Ikbal I, Wilson G, Ghiso J, Williams C, Refolo LM, Pappolla MA: Melatonin increases survival and inhibits oxidative and amyloid pathology in a transgenic model of Alzheimer’s disease. J Neurochem 85: 1101-1108, 2003 Pappolla MA, Bryant-Thomas TK, Herbert D, Pacheco J, Fabra Garcia M, Manjon M, Girones X, Henry TL, Matsubara E, Zambon D, Wolozin B, Sano M, Cruz-Sanchez FF, Thal LJ, Petanceska SS, Refolo LM: Mild hypercholesterolemia is an early risk factor for the development of Alzheimer amyloid pathology. Neurology 61: 199-205, 2003 Matsubara E, Sekijima Y, Tokuda T, Urakami K, Amari M, Shizuka-Ikeda M, Tomidokoro Y, Ikeda M, Kawarabayashi T, Harigaya Y, Ikeda S, Murakami T, Abe K, Otomo E, Hirai S, Frangione B, Ghiso J, Shoji M: Soluble Aβ homeostasis in AD and DS: impairment of anti-amyloidogenic protection by lipoproteins. Neurobiol Aging 25: 833-841, 2004 Kawarabayashi T, Shoji M, Younkin L, Wen-Lang L, Dickson DW, Murakami T, Matsubara E, Abe K, Ashe KH, Younkin SG: Dimeric Aβ rapidly accumulates in lipid rafts followed by ApoE and phosphorylated tau as memory is impaired in the Tg2576 mouse model of Alzheimer’s disease. J Neurosci 24: 3801-3809, 2004 Murakami T, Shoji-M, Imai Y, Inoue H, KawarabayashiT, Matsubara E, Harigaya Y, SasakiA, Takahashi R, AbeK: Pael-R is accumulated in Lewy bodies of Parkinson’s disease. Ann Neurol 55: 439-442, 2004 Ikarashi Y, Harigaya Y, Tomidokoro Y, Kanai M, Ikeda M, Matsubara E, Kawarabayashi T, Kuribara H, Younkin SG, Shoji M: Decreased level of brain acetylcholine and memory disturbance in APPsw mice. Neurobiol Aging 25: 483-490, 2004 Kurata T, Matsubara E, Yokoyama M, Nagano I, Shoji M, Abe K: Improvement of SSPE by intrathecal infusion of α-IFN. Neurology 63: 398-399, 2004 Yamashita T, Matsubara E, Nagano I, Shoji M, Abe K: Bilateral extraocular muscle atrophy in myotonic dystrophy type 1. Neurology 63: 759-760, 2004 Ikeda M, Shoji M, Kawarai T, Kawarabayashi T, Matsubara E, Murakami T, Sasaki A, Tomidokoro Y, Ikarashi Y, Kuribara H, Ishiguro K, Hasegawa M, Yen SH, Davies P, Chishti MA, Harigaya Y, Okamoto K, Abe K, Carlson GA, St.George-Hyslop P, Westaway D: Accumulation of Filamentous Tau in the Cerebral Cortex of Human Tau R406W Transgenic Mice. Am J Pathol 166: 521-531, 2005 Matsubara E, Kurata T, Yokoyama M, Nagano I, Shoji M, Abe K: Improvement of SSPE by intrathecal infusion of α-IFN. Neurology 64: 402, 2005 Nakaya Y, Yamane T, Shiraishi H, Wang HQ, Matsubara E, Sato T, Dolios G, Wang R, De Strooper B, Shoji M, Komano H, Yanagisawa K, Ihara Y, Fraser P, St George-Hyslop P, Nishimura M: Random mutagenesis of presenilin-1 identifies novel mutants exclusively generating long amyloid beta-peptides. J Biol Chem 280: 19070-19077, 2005 Matsubara E, Nagata T, Kageyama Y, Shiote M, Namba R, Nagano I, Shoji M, Abe K: Unique cerebellar-cerebral form of autosomal recessive ataxia. Tohoku J Exp Med 207: 81-85, 2005 水口　峰之　（富山医科薬科大学・薬学部） Tada M, Aizawa T, Shinohara Y, Matsubara K, Miura K, Yoshida M, Shitara K, Kouno T, Mizuguchi M, Nitta K, Hayakawa Y, Kawano K: Role of aromatic residues in the structure and biological activity of the small cytokine, growth-blocking peptide (GBP). J Biol Chem 278: 10778-10783, 2003 Matsubara K, Mizuguchi M, Kawano K: Expression of a synthetic gene encoding human transthyretin in Escherichia coli. Prot Expr Purif30: 55-61, 2003 Mizuguchi M, Kroon GJ, Wright PE, Dyson HJ: Folding of a β-sheet protein monitored by real-time NMR spectroscopy. J Mol Biol 328: 1161-1171, 2003 Sato K, Nakamura T, Mizuguchi M, Miura K, Tada M, Aizawa T, Gomi T, Miyamoto K, Kawano K: Solution structure of epiregulin and the effect of its C-terminal domain for receptor binding affinity. FEBS Lett 553: 232-238, 2003 Shinohara Y, Mizuguchi M, Matsubara K, Takeuchi M, Matsuura A, Aoki T, Igarashi K, Terada Y, Kawano K: Biophysical analyses of the transthyretin variants, Tyr114His and Tyr116Ser, associated with familial amyloidotic polyneuropathy. Biochemistry 42: 15053-15060, 2003 Kouno T, Mizuguchi M, Tanida I, Ueno T, Kominami K, Kawano K: Letter to the Editor : 1H 13C and 15N resonance assignments of human microtubule-associated protein light chain-3. J Biomol NMR 29: 415-416, 2004  Yoshida M, Aizawa T, Nakamura T, Shitara K, Hayakawa Y, Matsubara K, Miura K, Kouno T, Clark KD, Strand MR, Mizuguchi M, Demura M, Nitta K, Kawano K: The Gly-Gly linker region of the insect cytokine growth blocking peptide (GBP) is essential for activity. J Biol Chem 279: 51331-51337, 2004  Nakamura T, Takasugi H, Aizawa T, Yoshida M, Mizuguchi M, MoriY, Shinoda H, Hayakawa Y, Kawano K: Peptide mimics of epidermal growth factor (EGF) with antagonistic activity. J Biotechnol 116: 211-219, 2005 Matsubara K, Mizuguchi M, Igarashi K, Shinohara Y, Takeuchi M, Matsuura A, Saitoh T, Mori Y, Shinoda H, Kawano K: Dimeric transthyretin variant assembles into spherical neurotoxins. Biochemistry 44: 3280-3288, 2005 満田　憲昭　（愛媛大学・医学部） Tamatani M, Mitsuda N, Matsuzaki H, Okado H, Miyake S, Vitek MP, Yamaguchi A Tohyama M: A pathway of neuronal apoptosis induced by hypoxia/reoxygenation: roles of NFκB and Bcl-2. J Neurochem 75: 683-693, 2000 Hatanaka Y, Kamino K, Fukuo K, Mitsuda N, Nishiwaki-Ueda Y, Sato N, Satoh T, Yamamoto H, Yoneda H, Imagawa M, Miki T, Ohta S, Ogihara T: Low density lipoprotein receptor-related protein gene polymorphisms and risk for late-onset Alzheimer's disease in a Japanese population. Clin Genet 58: 319-323, 2000 Tamatani M, Matsuyama T, Yamaguchi A, Mitsuda N, Tsukamoto Y, Taniguchi M, Che YH, Ozawa K, Hori O, Nishimura H, Yamashita A, Okabe M, Yanagi H, Stern DM, Ogawa S, Tohyama M: ORP150 protects against hypoxia/ischemia-induced neuronal death. Nat Med 7: 317-323, 2001 Mitsuda N, Ohkubo N, Tamatani M, Lee YD, Taniguchi M, Namikawa K, Kiyama H, Yamaguchi A, Sato N, Ogihara T, Vitek MP, Tohyama M: Activated CREB regulates neuronal expression of presenilin-1. J Biol Chem 276: 9688-9698, 2001 Matsuzaki H, Tamatani M, Yamaguchi A, Namikawa K, Kiyama H, Vitek MP, Mitsuda N, Tohyama M: Vascular endothelial growth factor rescues hippocampal neurons from glutamate-induced toxicity: signal transduction cascades. FASEB J 15: 1218-1220, 2001 Yamaguchi A, Tamatani M, Mitsuda N, Matsuzaki H, Vitek MP, Tohyama M: Akt activation protects hippocampal neurons from apoptosis by inhibiting transcriptional activity of p53. J Biol Chem 276: 5256-5264, 2001 Ohkubo N, Mitsuda N, Tamatani M, Yamaguchi A, Lee Y-D, Ogihara T, Vitek MP, Tohyama M: Apolipoprotein E4 stimulates CREB’s transcriptional activity through the ERK pathway. J Biol Chem 276: 3046-3053, 2001 Matsubara-Tsutsui M, Yamagata H, Morishima A, Nakura J, Mitsuda N, Kamino K, Kondo I, Miki T: The 4,752 C/T polymorphism in the presenilin 1 gene increases the risk of Alzheimer’s disease in apolipoprotein E4 carriers. Intern Med 41: 823-828, 2002 Mori M, Nakagami H, Morishita R, Mitsuda N, Yamamoto K, Yoshimura S, Ohkubo N, Sato N, Ogihara T, Kaneda Y: N141I mutant presenilin-2 gene enhances neuronal cell death and decreases bcl-2 expression. Life Sci 70: 2567-2580, 2002 Miyake S, Yamashita T, Taniguchi M, Tamatani M, Sato K, Kawai Y, Senba E, Mitsuda N, Hori O, Yamaguchi A, Tohyama M: Expression of mitochondrial tricarboxylate carrier TCC mRNA and protein in the rat brain. Mol Brain Res 100: 67-73, 2002 武藤　多津郎　（福井医科大学（現・福井大学）・医学部） Mutoh T, Sobue G, Hamano T, Kuriyama M, Hirayama M, Yamamoto M, Mitsuma T: Decreased tyrosine phosphorylation of TrkB receptor in the spinal cords from the patients with amyotrophic lateral sclerosis. Neurochem Res 25: 239-245, 2000 Hamano T, Mutoh T, Sugie H, Koga H, Kuriyama M: Phosphoglycerate kinase deficiency. An adult myopathic form with a novel mutation. Neurology 54: 1188-1190, 2000 Kumano T, Mutoh T, Nakagawa H, Kuriyama M: HMG-CoA reductase inhibitor induces a transient activation of high affinity nerve growth factor receptor, Trk, and morphological differentiation with fatal outcome in PC12 cells. Brain Res 859: 169-172, 2000 Fukumoto S, Mutoh T, Hasegawa T, Miyazaki H, Okada M, Goto G, Furukawa K, Urano T, Furukawa K: GD3 synthase gene expression in PC12 cells resulted in the continuous activation of TrkA and ERK1/2, leading to enhanced proliferation. J Biol Chem 275: 5832-5838, 2000 Hamano T, Miyoshi Y, Hirayama M, Hiraki S, Mutoh T, Kuriyama M: Posterior thoracic spinal cord infarction: Complication of thoracoabdominal aortic aneurysm. Eur Neurol 44: 59-60, 2000 Mutoh T, Hamano T, Tokuda A, Kuriyama M: Unglycosylated Trk protein does not co-localize nor associate with gangliosides GM1 in stable clone overexpressing Trk of PC12 cells (PCtrk). Glycoconj J 17: 233-237, 2000 Nakagawa H, Mutoh T, Kumano T, Kuriyama M: Tyrosine phosphorylation of the catalytic subunit p110 of phosphatidylinositol-3 kinase induced by HMG-CoA reductase inhibitor in L6 myoblasts. FEBS Lett 508: 53-56, 2001 Hirayama M, Mutoh T, Tokuda A: Acid hydrolase activity of cultured bovine oligodendrocytes. Neurochem Res 26: 121-124, 2001 村山　繁雄　（東京都老人総合研究所） Saito Y, Kawashima A, Fujiwara H, Ruberu NN, Hasegawa M, Iwatsubo T, Murayama S: Accumulation of phosphorylated α-synuclein in aging human brain. J Neuropathol Exp Neurol 62: 644-654, 2003 Saito Y, Ruberu NN, Harada M, Arai T, Sawabe M, Nukina N, Murayama S: In-situ detection of apolipoprotein E ε4 in archival human brain. NeuroReport 15: 1113-1115, 2004 Ruberu NN, Saito Y, Honma N, Sawabe M, Yamanouchi H Murayama S: Granulomatous meningitis as a late complication of iodized oil myelography. Neuropathology 24: 144-148, 2004 Saito Y, Suzuki K, Huelette C, Murayama S: Aberrant phosphorylation of alpha-synuclein in human Niemann-Pick type C1 disease. J Neuropath Exp Neurol 63: 323-328, 2004 Kazama H, Ruberu NN, Murayama S, Saito Y, Nakahara K, Kanemaru K, Nagura H, Arai T, Sawabe M, Yamanouchi H, Orimo H, Hosoi T: Association of estrogen receptor alpha-gene polymorphisms with neurofibrillary tangles. Dement Geriatr Cogn Disord 18: 145-150, 2004 Saito Y, Ruberu NN, Sawabe M, Arai T, Kazama H, Hosoi T, Yamanouchi H Murayama S: Lewy body-related alpha-synucleinopathy in aging. J Neuropathol Exp Neurol 63: 742-749, 2004 Sawabe M, Arai T, Kasahara I, Esaki Y, Nakahara K, Hosoi T, Orimo H, Takubo K, Murayama S, Tanaka N: Development of a geriatric autopsy database and internet-based database of Japanese single nucleotide polymorpisms for geriatric research (JG-SNP). Mech Aging Dev 125: 547-552, 2004 Saito Y, Ruberu NN, Sawabe M, Arai T, Tanaka N, Kakuta Y, Yamanouchi H, Murayama S: Staging of argyrophilic grains, an age-associated tauopathy. J Neuropathol Exp Neurol 63: 911-918, 2004 Murayama S, Saito Y: europathological diagnostic criteria for Alzheimer disease. Neuropathology 24: 254-260, 2004 Hamaguchi T, Kitamoto T, Sato T, Mizusawa H, Nakamura Y, Noguchi M, Furukawa Y, Ishida C, Kuji I, Mitani K, Murayama S, Kohriyama T, Katayama S, Yamashita M, Yamamoto T, Udaka F, Kawakami A, Ihara Y, Nishinaka T, Kuroda S, Suzuki N, Shiga Y, Arai H, Maruyama M, Yamada M: Clinical diagnosis of MM2-type sporadic Creutzfeldt-Jakob disease. Neurology 64: 643-648, 2004 Katsuno T, Morishima-Kawashima M, Saito Y, Yamanouchi H, Ishiura S, Murayama S, Ihara Y: Independent accumulations of tau and amyloid β-protein in the human entorhinal cortex. Neurology 64: 687-692, 2005 Ishigami A, Ohsawa T, Hiratsuka M, Taguchi H, Kobayashi S, Saito Y, Murayama S, Asaga H, Toda T, Kimura N, Maruyama N: Abnormal accumulation of citrullinated proteins catalyzed by peptidylarginine deiminase in hippocampal extracts from patients with Alzheimer’s disease. Neurosci Res 80: 120-128, 2005 門司　晃　（九州大学・大学病院・精神科神経科） Hashioka S, Monji A, Ueda T, Kanba S, Nakanishi H: Amyloid-beta fibril formation is not necessarily required for microglial activation by the peptides. Neurochem Int 47: 369-376, 2005 山口　晴保　（群馬大学・医学部・保健学科） Yamaguchi H, Maat-Schieman ML, van Duinen SG, Prins FA, Neeskens P, Natte R, Roos RA: Amyloid β protein (Aβ) starts to deposit as plasma membrane-bound form in diffuse plaques of brains from hereditary cerebral hemorrhage with amyloidosis-Dutch type, Alzheimer disease and nondemented aged subjects. J Neuropathol Exp Neurol 59: 723-732, 2000 Morishima-Kawashima M, Oshima N, Ogata H, Yamaguchi H, Yoshimura M, Sugihara S, lhara Y: Effect of apolipoprotein E allel ε4 on the initial phase of amyloid β-protein accumulation in the human brain. Am J Pathol 157: 2093-2099, 2000 Thal DR, Schultz C, Dehghani F, Yamaguchi H, Braak H, Braak E: Amyloid β-protein (Aβ)-containing astrocytes are located preferentially near N-terminal-truncated Aβ deposits in the human entorhinal cortex. Acta Neuropathol 100: 608-617, 2000 Kokubo H, Lemere CA, Yamaguchi H: Localization of flotillins in human brain and their accumulation with the progression of Alzheimer's disease pathology. Neurosci Lett 290: 93-96, 2000 Tsuzuki K, Fukatsu R, Yamaguchi H, Tateno M, Imai K, Fujii N, Yamauchi T: Transthyretin binds amyloid β peptides, Aβ1-42 and Aβ1-40 to form complex in the autopsied human kidney-possible role of transthyretin for Aβ sequestration. Neurosci Lett 281: 171-174, 2000 Fakla I, Kovacs I, Yamaguchi H, Geula C, Kasa P: Expressions of amyloid precursor protein, synaptophysin and presenilin-1 in the different areas of the developing cerebellum of rat. Neurochem Int 36: 143-151, 2000 Kasa P, Papp H, Kovacs I, Forgon M, Penke B, Yamaguchi H: Human amyloid-β1-42 applied in vivo inhibits the fast axonal transport of proteins in the sciatic nerve of rat. Neurosci Lett 278: 117-119, 2000 Maat-Schieman ML, Yamaguchi H, van Duinen SG, Natte R, Roos RA: Age-related plaque morphology and C-terminal heterogeneity of amyloid β in Dutch-type hereditary cerebral hemorrhage with amyloidosis. Acta Neuropathol 99: 409-419, 2000 Yamaguchi H, Sugihara S, Ogawa A, Ihara Y: Alzheimer β amyloid deposition, which is hastened by ApoE ε4 gene, precedes neurofibrillary pathology in the frontal association cortex of non-demented senior subjects. J Neuropathol Exp Neurol 60: 731-739, 2001 Oshima N, Morishima-Kawashima M, Yamaguchi H, Yoshimura M, Sugihara S, Khan K, Games D, Schenk D, Ihara Y: Accumulation of amyloid β-protein in the low-density membrane domain accurately reflects the extent of β-amyloid deposition in the brain. Am J Pathol 158: 2209-2218, 2001 Tanemura K, Akagi T, Murayama M, Kikuchi N, Murayama O, Hashikawa T, Yoshiike Y, Park J-M, Matsuda K, Nakao S, Sun X, Sato S, Yamaguchi H, Takashima A: Formation of filamentous tau aggregations in transgenic mice expressing V337M human tau. Neurobiol Dis 8: 1036-1045, 2001 Sun X, Cole GM, Chu T, Xia W, Galasko D, Yamaguchi H, Tanemura K, Frautschy A, Takashima A: Intracellular Aβ is increased by okadaic acid exposure in transfected neuronal and non-neuronal cell lines. Neurobiol Aging 23: 195-203, 2002 Sai X, Kawamura Y, Kokame K, Yamaguchi H, Shiraishi H, Suzuki R, Suzuki T, Kawaichi M, Miyata T, Kitamura T, De Strooper B, Yanagisawa K, Komano H: Endoplasmic reticulum stress-inducible protein, Herp, enhances presenilin-mediated generation of amyloid β-protein. J. Biol. Chem 277: 12915-12920, 2002 Tanemura K, Murayama M, Akagi T, Hashikawa T, Tominaga T, Ichikawa M, Yamaguchi H, Takashima A: Neurodegeneration with tau accumulation in a transgenic mouse expressing V337M human tau. J Neurosci 22: 133-141, 2002 Sun X, Sato S, Murayama O, Murayama M, Park JM, Yamaguchi H, Takashima A: Lithium inhibits amyloid secretion in COS7 cells transfected with amyloid precursor protein C100. Neurosci Lett 321: 61-64, 2002 Thal DR, Ghebremedhin E, Rub U, Yamaguchi H, Tredici KD, Braak H: Two types of sporadic cerebral amyloid angiopathy. J Neuropathol Exp Neurol 61: 282-293, 2002 Xia X, Wang P, Sun X, Soriano S, Shum WK, Yamaguchi H, Trumbauer ME, Takashima A, Koo H, Zheng H: The aspartate-257 of presenilin 1 is indispensable for mouse development and production of beta-amyloid peptides through beta-catenin-independent mechanisms. Proc Natl Acad Sci USA 99: 8760-8765, 2002 Takahashi RH, Milner TA, Li F, Nam EE, Edgar MA, Yamaguchi H, Beal MF, Xu H, Greengard P, Gouras GK: Intraneuronal Alzneimer Abeta42 accumulates in multivesicular bodies and is associated with synaptic pathology. Am J Pathol 161: 1869-1879, 2002 Sasaki A, Horikoshi Y, Yokoo H, Nakazato Y, Yamaguchi H: Antiserum against human glucose transporter 5 is highly specific for microglia among cells of the mononuclear phagocyte system. Neurosci Lett 338: 17-20, 2003 Horikoshi Y, Sasaki A, Taguchi N, Maeda M, Tsukagoshi H, Sato K, Yamaguchi H: Human GLUT5 immunolabeling is useful for evaluating microglial status in neuropathological study using paraffin sections. Acta Neuropathol 105: 157-162, 2003 Kokubo H, Helms J-B, Ohno-Iwashita Y, Shimada Y, Horikoshi Y, Yamaguchi H: Ultrastructural localization of flotillin-1 to cholesterol-rich membrane microdomains, rafts, in rat brain tissue. Brain Res 965: 83-90, 2003 Hashimoto N, Takeuchi T, Ishihara R, Ukai K, Kobayashi H, Iwata H, Iwai K, Mizuno Y, Yamaguchi H, Shibayama H: Glial fibrillary tangles in diffuse neurofibrillary tangles with calcification. Acta Neuropathol 106: 150-156, 2003 Kanamori T, Nishimaki K, Asoh S, Ishibashi Y, Takata I, Kuwabara T, Taira K,Yamaguchi H, Sugihara S, Yamazaki T, Ihara Y, Nakano K, Matuda S, Ohta S: Truncated product of the bifunctional DLST gene involved in biogenesis of the respiratory chain. EMBO J 22: 2913-2923, 2003 Rofina J, van Andel I, van Ederen AM, Papaoiannou N, Yamaguchi H, Gruys E: Canine counterpart of senile dementia of the Alzheimer type: amyloid plaques near capillaries but lack of spatial relationship with activated microglia and macrophages. Amyloid 10: 86-96, 2003 Araki Y, Tomita S, Yamaguti H, Miyagi N, Sumioka A, Kirino Y, Suzuki T: Novel cadherin-related membrane proteins, alcadeins, enhance the X11-like protein-mediated stabilization of amyloid β-protein. J Biol Chem 278: 49448-49458, 2003 Hayashi H, Kimura N, Yamaguchi H, Hasegawa K, Yokoseki T, Shibata M, Yamamoto N, Michikawa M, Yoshikawa Y, Terao K, Matsuzaki K, Lemere CA, Selkoe DJ, Naiki H, Yanagisawa K: A seed for Alzheimer amyloid in the brain. J Neurosci 24: 4894-4902, 2004 Horikoshi Y, Sakaguchi G, Becker AG, Gray AJ, Duff K, Aisen PS, Yamaguchi H, Maeda M, Kinoshita N, Matsuoka Y: Development of Abeta terminal end-specific antibodies and sensitive ELISA for Abeta variant. Biochem Biophys Res Commun 319: 733-737, 2004 Taguchi N, Hatabu T, Yamaguchi H, Suzuki M, Sato K, Kano S: Plasmodium falciparum: selenium-induced cytotoxicity to P. falciparum. Exp Parasitol 106: 50-55, 2004 Horikoshi Y, Mori T, Maeda M, Kinoshita N, Sato K, Yamaguchi H: Abeta N-terminal-end specific antibody reduced beta-amyloid in Alzheimer-model mice. Biochem Biophys Res Commun 325: 384-387, 2004 Maat-Schieman ML, Yamaguchi H, Hegeman-Kleinn IM, Welling-Graafland C, Natte R, Roos RA, van Duinen SG: Glial reactions and the clearance of amyloid beta protein in the brains of patients with hereditary cerebral hemorrhage with amyloidosis-Dutch type. Acta Neuropathol 107: 389-398, 2004 Sugiyama M, Ikeda E, Kawai S, Higuchi T, Zhang H, Khan N, Tomiyoshi K, Inoue T, Yamaguchi H, Katakura K, Endo K, Suzuki M: Cerebral metabolic reduction in severe malaria: fluorodeoxyglucose-positron emission tomography imaging in a primate model of severe human malaria with cerebral involvement. Am J Trop Med Hyg 71: 542-545, 2004 Sasaki A, Yamaguchi H, Horikoshi Y, Tanaka G, Nakazato Y: Expression of glucose transporter 5 by microglia in human gliomas. Neuropathol Appl Neurobiol 30: 447-455, 2004 Kokubo H, Saido TC, Iwata N, Helms JB, Shinohara R, Yamaguchi H: Part of membrane-bound Abeta exist in rafts within senile plaques in Tg2576 mouse brain. Neurobiol Aging 26: 409-418, 2005 Kokubo H, Kayed R, Glabe CG, Yamaguchi H: Soluble Abeta oligomers ultrastructurally localize to cell processes and might be related to synaptic dysfunction in Alzheimer’s disease brain. Brain Res 1031: 222-228, 2005 Kokubo H, Kayed R, Glabe CG, Saido TC, Iwata N, Helms JB, Yamaguchi H: Oligomeric proteins ultrastructurally localize to cell processes, especially to axon terminals with higher density, but not to lipid rafts in Tg2576 mouse brain. Brain Res 1045: 224-228, 2005 横山　信治　（名古屋市立大学・医学部） Ito J, Yokoyama S: Sialosylcholesterol induces reorganization of astrocyte filament network. 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J Biol Chem 277: 44709-44714, 2002 和栗　聡　（大阪大学・大学院医学系研究科） Koike M, Nakanishi H, Saftig P, Ezaki J, Isahara K, Ohsawa Y, Schulz-Schaeffer W, Watanabe T, Waguri S, Kametaka S, Shibata M, Yamamoto K, Kominami E, Peters C, von Figura K, Uchiyama Y: Cathepsin D deficiency induces lysosomal storage with ceroid lipofuscin in mouse CNS neurons. J Neurosci 20: 6898-6906, 2000 Waguri S, Kohmura M, Kanamori S, Watanabe T, Ohsawa Y, Koike M, Tomiyama Y, Wakasugi M, Kominami E, Uchiyama Y: Different distribution patterns of the two mannose 6-phosphate receptors in rat liver. J Histochem Cytochem 49: 1397-1405, 2001 Shibata M, Koike M, Waguri S, Zhang G, Koga T, Uchiyama Y: Cathepsin D is specifically inhibited by deoxyribonucleic acids. 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